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P47710 (CASA1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-S1-casein

Cleaved into the following chain:

  1. Casoxin-D
Gene names
Name:CSN1S1
Synonyms:CASA, CSN1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length185 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Important role in the capacity of milk to transport calcium phosphate.

Casoxin D acts as opioid antagonist and has vasorelaxing activity mediated by bradykinin B1 receptors.

Subunit structure

Heteromultimers of alpha-s1 casein and kappa-casein; disulfide-linked.

Subcellular location

Secreted.

Tissue specificity

Mammary gland specific. Secreted in milk.

Post-translational modification

Not glycosylated. Ref.12

Miscellaneous

In milk, the alpha s1- and beta-caseins precipitate in presence of calcium (so-called calcium-sensitive caseins). Kappa-casein prevents the precipitation of the other caseins by calcium through the formation of large stable colloidal particles termed micelles.

Sequence similarities

Belongs to the alpha-casein family.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   Molecular functionMilk protein
   PTMDisulfide bond
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontransporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P47710-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P47710-2)

The sequence of this isoform differs from the canonical sequence as follows:
     52-52: Missing.
Isoform 3 (identifier: P47710-3)

The sequence of this isoform differs from the canonical sequence as follows:
     66-73: Missing.
Isoform 4 (identifier: P47710-4)

The sequence of this isoform differs from the canonical sequence as follows:
     52-52: Missing.
     93-100: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515 Ref.8 Ref.9
Chain16 – 185170Alpha-S1-casein
PRO_0000004450
Peptide158 – 1647Casoxin-D
PRO_0000004451

Amino acid modifications

Modified residue311Phosphoserine Ref.11
Modified residue331Phosphoserine Ref.10 Ref.11 Ref.12
Modified residue411Phosphoserine Ref.10 Ref.11 Ref.12 Ref.14
Modified residue711Phosphoserine By similarity
Modified residue861Phosphoserine Ref.11
Modified residue881Phosphoserine Ref.10 Ref.11
Modified residue891Phosphoserine Ref.11
Modified residue901Phosphoserine Ref.10 Ref.11
Modified residue911Phosphoserine Ref.11

Natural variations

Alternative sequence521Missing in isoform 2 and isoform 4.
VSP_000795
Alternative sequence66 – 738Missing in isoform 3.
VSP_000796
Alternative sequence93 – 1008Missing in isoform 4.
VSP_046130
Natural variant1171A → V. Ref.6
Corresponds to variant rs10030475 [ dbSNP | Ensembl ].
VAR_048614

Experimental info

Sequence conflict801P → S in AAY68392. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: A7717899CDC7D563

FASTA18521,671
        10         20         30         40         50         60 
MRLLILTCLV AVALARPKLP LRYPERLQNP SESSEPIPLE SREEYMNGMN RQRNILREKQ 

        70         80         90        100        110        120 
TDEIKDTRNE STQNCVVAEP EKMESSISSS SEEMSLSKCA EQFCRLNEYN QLQLQAAHAQ 

       130        140        150        160        170        180 
EQIRRMNENS HVQVPFQQLN QLAAYPYAVW YYPQIMQYVP FPPFSDISNP TAHENYEKNN 


VMLQW 

« Hide

Isoform 2 [UniParc].

Checksum: 9427BB3C275D7B86
Show »

FASTA18421,543
Isoform 3 [UniParc].

Checksum: CF67025C444CC8CF
Show »

FASTA17720,740
Isoform 4 [UniParc].

Checksum: 1E03F910FA37C369
Show »

FASTA17620,693

References

« Hide 'large scale' references
[1]"Characterization of three types of human alpha s1-casein mRNA transcripts."
Johnsen L.B., Rasmussen L.K., Petersen T.E., Berglund L.
Biochem. J. 309:237-242(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
Tissue: Mammary gland.
[2]Yu D.Y., Jeong S., Lee K.K., Lonnerdal B.
Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Mammary gland.
[3]Lin L., Nong W., Zhou G., Ke R., Shen C., Zhong G., Zheng Z., Liang M., Wen S., Li H., Yang S.
Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
[4]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-117.
[7]"The gene encoding alpha s1-casein is expressed in human mammary epithelial cells during lactation."
Martin P., Brignon G., Furet J.-P., Leroux C.
Lait 76:525-537(1996)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-185 (ISOFORMS 1; 2 AND 3).
[8]"Human alpha S1-casein like protein: purification and N-terminal sequence determination."
Cavaletto M., Cantisani A., Giuffrida G., Napolitano L., Conti A.
Biol. Chem. Hoppe-Seyler 375:149-151(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 16-29.
[9]"Human alpha s1-casein: purification and characterization."
Rasmussen L.K., Due H.A., Petersen T.E.
Comp. Biochem. Physiol. 111B:75-81(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 16-29 AND 99-105, CHARACTERIZATION.
[10]"On studying protein phosphorylation patterns using bottom-up LC-MS/MS: the case of human alpha-casein."
Kjeldsen F., Savitski M.M., Nielsen M.L., Shi L., Zubarev R.A.
Analyst 132:768-776(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 27-42 AND 83-98, PHOSPHORYLATION AT SER-33; SER-41; SER-88 AND SER-90, IDENTIFICATION BY MASS SPECTROMETRY.
[11]"Analysis of the human casein phosphoproteome by 2-D electrophoresis and MALDI-TOF/TOF MS reveals new phosphoforms."
Poth A.G., Deeth H.C., Alewood P.F., Holland J.W.
J. Proteome Res. 7:5017-5027(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-48, PHOSPHORYLATION AT SER-31; SER-33; SER-41; SER-86; SER-88; SER-89; SER-90 AND SER-91, IDENTIFICATION BY MASS SPECTROMETRY.
[12]"The phosphorylation pattern of human alphas1-casein is markedly different from the ruminant species."
Sorensen E.S., Moller L., Vinther M., Petersen T.E., Rasmussen L.K.
Eur. J. Biochem. 270:3651-3655(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, PHOSPHORYLATION AT SER-33 AND SER-41, LACK OF GLYCOSYLATION, IDENTIFICATION BY MASS SPECTROMETRY.
[13]"Genomic organization and chromosomal localization of the human casein gene family."
Fujiwara Y., Miwa M., Nogami M., Okumura K., Nobori T., Suzuki T., Ueda M.
Hum. Genet. 99:368-373(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOMIC ORGANIZATION, CHROMOSOMAL LOCATION.
[14]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Web resources

Protein Spotlight

Of buttons, digestion and glue - Issue 16 of November 2001

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X78416 mRNA. Translation: CAA55185.1.
U23157 mRNA. Translation: AAA69477.1.
DQ064604 mRNA. Translation: AAY68392.1.
AC108941 Genomic DNA. No translation available.
CH471057 Genomic DNA. Translation: EAX05599.1.
BC128227 mRNA. Translation: AAI28228.1.
BC128228 mRNA. Translation: AAI28229.1.
X98084 mRNA. Translation: CAA66708.1.
PIRS56013.
RefSeqNP_001020275.1. NM_001025104.1.
NP_001881.1. NM_001890.1.
UniGeneHs.3155.

3D structure databases

ProteinModelPortalP47710.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107833. 12 interactions.
IntActP47710. 2 interactions.
STRING9606.ENSP00000246891.

Protein family/group databases

Allergome1064. Hom s 8.

PTM databases

PhosphoSiteP47710.

Proteomic databases

PaxDbP47710.
PRIDEP47710.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000246891; ENSP00000246891; ENSG00000126545. [P47710-1]
ENST00000444405; ENSP00000413157; ENSG00000126545. [P47710-4]
ENST00000507763; ENSP00000422611; ENSG00000126545. [P47710-4]
GeneID1446.
KEGGhsa:1446.
UCSCuc003hep.1. human. [P47710-1]
uc003heq.1. human.

Organism-specific databases

CTD1446.
GeneCardsGC04P070845.
HGNCHGNC:2445. CSN1S1.
HPAHPA035659.
MIM115450. gene.
neXtProtNX_P47710.
PharmGKBPA26948.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG73245.
HOGENOMHOG000115954.
HOVERGENHBG005242.
InParanoidP47710.
KOK17281.
OMASKCAEQF.
OrthoDBEOG7W1561.
PhylomeDBP47710.
TreeFamTF340763.

Gene expression databases

ArrayExpressP47710.
BgeeP47710.
CleanExHS_CSN1S1.
GenevestigatorP47710.

Family and domain databases

InterProIPR026999. Alpha-s1_casein.
IPR001588. Casein.
[Graphical view]
PANTHERPTHR10240. PTHR10240. 1 hit.
PROSITEPS00306. CASEIN_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCSN1S1.
GenomeRNAi1446.
NextBio5941.
PMAP-CutDBP47710.
PROP47710.
SOURCESearch...

Entry information

Entry nameCASA1_HUMAN
AccessionPrimary (citable) accession number: P47710
Secondary accession number(s): A1A510 expand/collapse secondary AC list , A1A511, E9PB60, Q4PNR5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM