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Protein

Rabphilin-3A

Gene

Rph3a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi409 – 4091Calcium 1; via carbonyl oxygenCombined sources1 Publication
Metal bindingi410 – 4101Calcium 1Combined sources1 Publication
Metal bindingi410 – 4101Calcium 2Combined sources1 Publication
Metal bindingi416 – 4161Calcium 2Combined sources1 Publication
Metal bindingi471 – 4711Calcium 1Combined sources1 Publication
Metal bindingi471 – 4711Calcium 2Combined sources1 Publication
Metal bindingi472 – 4721Calcium 2; via carbonyl oxygenCombined sources1 Publication
Metal bindingi473 – 4731Calcium 1Combined sources1 Publication
Metal bindingi473 – 4731Calcium 2Combined sources1 Publication
Metal bindingi479 – 4791Calcium 1Combined sources1 Publication
Metal bindingi526 – 5261Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi568 – 5681Calcium 3By similarity
Metal bindingi568 – 5681Calcium 4By similarity
Metal bindingi574 – 5741Calcium 3By similarity
Metal bindingi628 – 6281Calcium 3By similarity
Metal bindingi628 – 6281Calcium 4By similarity
Metal bindingi629 – 6291Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi630 – 6301Calcium 3By similarity
Metal bindingi630 – 6301Calcium 4By similarity
Metal bindingi636 – 6361Calcium 4By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri88 – 14558FYVE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Calcium, Lipid-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Rabphilin-3A
Alternative name(s):
Exophilin-1
Gene namesi
Name:Rph3a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:102788. Rph3a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 681681Rabphilin-3APRO_0000190228Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei271 – 2711PhosphoserineCombined sources
Modified residuei679 – 6791PhosphoserineBy similarity
Modified residuei680 – 6801PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47708.
PaxDbiP47708.
PeptideAtlasiP47708.
PRIDEiP47708.

PTM databases

iPTMnetiP47708.
PhosphoSiteiP47708.

Expressioni

Tissue specificityi

Specifically expressed in brain.

Gene expression databases

BgeeiENSMUSG00000029608.
CleanExiMM_RPH3A.
ExpressionAtlasiP47708. baseline and differential.
GenevisibleiP47708. MM.

Interactioni

Subunit structurei

Monomer. Interacts with RAB3A, RAB3B, RAB3C, RAB3D, RAB8A, RAB27A and RAB27B.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rab27aQ9ERI22EBI-398376,EBI-398172
Rab3aP630112EBI-398376,EBI-398393
Rab8aP552582EBI-398376,EBI-398411

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202964. 9 interactions.
IntActiP47708. 7 interactions.
MINTiMINT-4132964.
STRINGi10090.ENSMUSP00000078198.

Structurei

Secondary structure

1
681
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi382 – 3909Combined sources
Turni391 – 3944Combined sources
Beta strandi395 – 40410Combined sources
Beta strandi417 – 42610Combined sources
Turni428 – 4303Combined sources
Beta strandi432 – 4376Combined sources
Beta strandi445 – 4539Combined sources
Helixi457 – 4604Combined sources
Beta strandi464 – 4729Combined sources
Beta strandi474 – 4763Combined sources
Beta strandi478 – 48710Combined sources
Helixi488 – 4903Combined sources
Beta strandi495 – 5028Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2K3HNMR-A368-507[»]
ProteinModelPortaliP47708.
SMRiP47708. Positions 44-167, 379-677.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP47708.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 157118RabBDPROSITE-ProRule annotationAdd
BLAST
Domaini381 – 485105C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini539 – 642104C2 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi277 – 36185Pro-richAdd
BLAST

Domaini

Binds calcium via the C2 domains. The calcium-bound C2 domains mediate interactions with phospholipid bilayers.

Sequence similaritiesi

Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 1 RabBD (Rab-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri88 – 14558FYVE-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1013. Eukaryota.
ENOG410XQXA. LUCA.
GeneTreeiENSGT00810000125376.
HOGENOMiHOG000294226.
HOVERGENiHBG017739.
InParanoidiP47708.
KOiK19938.
OMAiMLGSACV.
OrthoDBiEOG091G0FPP.
PhylomeDBiP47708.
TreeFamiTF351844.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR010911. Rab_BD.
IPR028698. RPH3A.
IPR001565. Synaptotagmin.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10024:SF118. PTHR10024:SF118. 1 hit.
PfamiPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50916. RABBD. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47708-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTVVNRWM YPGDGPLQSN DKEQLQAGWS VHPGAQTDRQ RKQEELTDEE
60 70 80 90 100
KEIINRVIAR AEKMEAMEQE RIGRLVDRLE TMRKNVAGDG VNRCILCGEQ
110 120 130 140 150
LGMLGSACVV CEDCKKNVCT KCGVETSNNR PHPVWLCKIC LEQREVWKRS
160 170 180 190 200
GAWFFKGFPK QVLPQPMPIK KTKPQQPAGE PATQEQPTPE SRHPARAPAR
210 220 230 240 250
GDMEDRRPPG QKPGPDLTSA PGRGSHGPPT RRASEARMST AARDSEGWDH
260 270 280 290 300
AHGGGTGDTS RSPAGLRRAN SVQAARPAPA PVPSPAPPQP VQPGPPGGSR
310 320 330 340 350
ATPGPGRFPE QSTEAPPSDP GYPGAVAPAR EERTGPAGGF QAAPHTAAPY
360 370 380 390 400
SQAAPARQPP PAEEEEEEAN SYDSDEATTL GALEFSLLYD QDNSNLQCTI
410 420 430 440 450
IRAKGLKPMD SNGLADPYVK LHLLPGASKS NKLRTKTLRN TRNPVWNETL
460 470 480 490 500
QYHGITEEDM QRKTLRISVC DEDKFGHNEF IGETRFSLKK LKANQRKNFN
510 520 530 540 550
ICLERVIPMK RAGTTGSARG MALYEEEQVE RIGDIEERGK ILVSLMYSTQ
560 570 580 590 600
QGGLIVGIIR CVHLAAMDAN GYSDPFVKLW LKPDMGKKAK HKTQIKKKTL
610 620 630 640 650
NPEFNEEFFY DIKHSDLAKK SLDISVWDYD IGKSNDYIGG CQLGISAKGE
660 670 680
RLKHWYECLK NKDKKIERWH QLQNENHVSS D
Length:681
Mass (Da):75,489
Last modified:June 6, 2002 - v2
Checksum:iD09F8D8D2CBB271E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC042585 mRNA. Translation: AAH42585.1.
BC050883 mRNA. Translation: AAH50883.1.
D29965 mRNA. Translation: BAA06231.2.
CCDSiCCDS39246.1.
PIRiJX0338.
RefSeqiNP_001289273.1. NM_001302344.1.
NP_001289274.1. NM_001302345.1.
NP_035416.1. NM_011286.3.
UniGeneiMm.181166.

Genome annotation databases

EnsembliENSMUST00000079204; ENSMUSP00000078198; ENSMUSG00000029608.
ENSMUST00000202326; ENSMUSP00000144291; ENSMUSG00000029608.
ENSMUST00000202406; ENSMUSP00000143917; ENSMUSG00000029608.
GeneIDi19894.
KEGGimmu:19894.
UCSCiuc008zil.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC042585 mRNA. Translation: AAH42585.1.
BC050883 mRNA. Translation: AAH50883.1.
D29965 mRNA. Translation: BAA06231.2.
CCDSiCCDS39246.1.
PIRiJX0338.
RefSeqiNP_001289273.1. NM_001302344.1.
NP_001289274.1. NM_001302345.1.
NP_035416.1. NM_011286.3.
UniGeneiMm.181166.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2K3HNMR-A368-507[»]
ProteinModelPortaliP47708.
SMRiP47708. Positions 44-167, 379-677.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202964. 9 interactions.
IntActiP47708. 7 interactions.
MINTiMINT-4132964.
STRINGi10090.ENSMUSP00000078198.

PTM databases

iPTMnetiP47708.
PhosphoSiteiP47708.

Proteomic databases

MaxQBiP47708.
PaxDbiP47708.
PeptideAtlasiP47708.
PRIDEiP47708.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079204; ENSMUSP00000078198; ENSMUSG00000029608.
ENSMUST00000202326; ENSMUSP00000144291; ENSMUSG00000029608.
ENSMUST00000202406; ENSMUSP00000143917; ENSMUSG00000029608.
GeneIDi19894.
KEGGimmu:19894.
UCSCiuc008zil.2. mouse.

Organism-specific databases

CTDi22895.
MGIiMGI:102788. Rph3a.

Phylogenomic databases

eggNOGiKOG1013. Eukaryota.
ENOG410XQXA. LUCA.
GeneTreeiENSGT00810000125376.
HOGENOMiHOG000294226.
HOVERGENiHBG017739.
InParanoidiP47708.
KOiK19938.
OMAiMLGSACV.
OrthoDBiEOG091G0FPP.
PhylomeDBiP47708.
TreeFamiTF351844.

Miscellaneous databases

ChiTaRSiRph3a. mouse.
EvolutionaryTraceiP47708.
PROiP47708.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029608.
CleanExiMM_RPH3A.
ExpressionAtlasiP47708. baseline and differential.
GenevisibleiP47708. MM.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR010911. Rab_BD.
IPR028698. RPH3A.
IPR001565. Synaptotagmin.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10024:SF118. PTHR10024:SF118. 1 hit.
PfamiPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50916. RABBD. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRP3A_MOUSE
AccessioniPrimary (citable) accession number: P47708
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: June 6, 2002
Last modified: September 7, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.