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P47701 (RSMA_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase A

EC=2.1.1.182
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase
16S rRNA dimethyladenosine transferase
16S rRNA dimethylase
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase
Gene names
Name:rsmA
Synonyms:ksgA
Ordered Locus Names:MG463
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity. HAMAP-Rule MF_00607

Catalytic activity

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. HAMAP-Rule MF_00607

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00607.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 259259Ribosomal RNA small subunit methyltransferase A HAMAP-Rule MF_00607
PRO_0000101559

Sites

Binding site131S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site151S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site401S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site611S-adenosyl-L-methionine By similarity
Binding site851S-adenosyl-L-methionine By similarity
Binding site1051S-adenosyl-L-methionine By similarity

Experimental info

Sequence conflict243 – 2486SPCQLF → MSLSTL Ref.2

Sequences

Sequence LengthMass (Da)Tools
P47701 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: DE67A0F2C28DACCA

FASTA25929,954
        10         20         30         40         50         60 
MNSFFPSRKL GQNFTVNLSV IKRIFAFVKN LNPQAIVEIG VGKGALTNYL LKLKIPYKGI 

        70         80         90        100        110        120 
EIDKRLIEYL LVEKILTEDQ LVKGDILKKD FNSFFENLSP LLCGNIPYSI TSPIINKFLE 

       130        140        150        160        170        180 
SKLRSFVLMT QKEFANRLLA KVNSSDYSAF GAFCQYYLTI TTVFKIDRHA FKPKPKVDST 

       190        200        210        220        230        240 
LILLEKNKSV SYDFKFGLFL KQCFNQRRKM LINNLKHFFA VDYLLNIIQK QNLKTSIRAQ 

       250 
ELSPCQLFNL YQNICNGKN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC72483.1.
U01719 Unassigned DNA. Translation: AAC43194.1.
PIRB64251.
RefSeqNP_073133.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47701.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_463.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC72483; AAC72483; MG_463.
GeneID875591.
KEGGmge:MG_463.
PATRIC20010520. VBIMycGen98045_0533.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0030.
KOK02528.
OMAILMVQNE.
OrthoDBEOG66F08Z.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-516-MONOMER.

Family and domain databases

Gene3D1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPMF_00607. 16SrRNA_methyltr_A.
InterProIPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR11727. PTHR11727. 1 hit.
PfamPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00755. ksgA. 1 hit.
PROSITEPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRSMA_MYCGE
AccessionPrimary (citable) accession number: P47701
Secondary accession number(s): Q49194
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names