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P47701

- RSMA_MYCGE

UniProt

P47701 - RSMA_MYCGE

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Protein

Ribosomal RNA small subunit methyltransferase A

Gene

rsmA

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.UniRule annotation

Catalytic activityi

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei15 – 151S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei40 – 401S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei61 – 611S-adenosyl-L-methionineUniRule annotation
Binding sitei85 – 851S-adenosyl-L-methionineUniRule annotation
Binding sitei105 – 1051S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

  1. 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity Source: UniProtKB-EC
  2. RNA binding Source: UniProtKB-KW
  3. rRNA (adenine-N6,N6-)-dimethyltransferase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-516-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase AUniRule annotation (EC:2.1.1.182UniRule annotation)
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferaseUniRule annotation
16S rRNA dimethyladenosine transferaseUniRule annotation
16S rRNA dimethylaseUniRule annotation
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation
Gene namesi
Name:rsmAUniRule annotation
Synonyms:ksgAUniRule annotation
Ordered Locus Names:MG463
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259Ribosomal RNA small subunit methyltransferase APRO_0000101559Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243273.MG_463.

Structurei

3D structure databases

ProteinModelPortaliP47701.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0030.
KOiK02528.
OMAiILMVQNE.
OrthoDBiEOG66F08Z.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A.
InterProiIPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47701-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSFFPSRKL GQNFTVNLSV IKRIFAFVKN LNPQAIVEIG VGKGALTNYL
60 70 80 90 100
LKLKIPYKGI EIDKRLIEYL LVEKILTEDQ LVKGDILKKD FNSFFENLSP
110 120 130 140 150
LLCGNIPYSI TSPIINKFLE SKLRSFVLMT QKEFANRLLA KVNSSDYSAF
160 170 180 190 200
GAFCQYYLTI TTVFKIDRHA FKPKPKVDST LILLEKNKSV SYDFKFGLFL
210 220 230 240 250
KQCFNQRRKM LINNLKHFFA VDYLLNIIQK QNLKTSIRAQ ELSPCQLFNL

YQNICNGKN
Length:259
Mass (Da):29,954
Last modified:February 1, 1996 - v1
Checksum:iDE67A0F2C28DACCA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti243 – 2486SPCQLF → MSLSTL(PubMed:8253680)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC72483.1.
U01719 Unassigned DNA. Translation: AAC43194.1.
PIRiB64251.
RefSeqiNP_073133.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC72483; AAC72483; MG_463.
GeneIDi875591.
KEGGimge:MG_463.
PATRICi20010520. VBIMycGen98045_0533.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC72483.1 .
U01719 Unassigned DNA. Translation: AAC43194.1 .
PIRi B64251.
RefSeqi NP_073133.1. NC_000908.2.

3D structure databases

ProteinModelPortali P47701.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243273.MG_463.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC72483 ; AAC72483 ; MG_463 .
GeneIDi 875591.
KEGGi mge:MG_463.
PATRICi 20010520. VBIMycGen98045_0533.

Phylogenomic databases

eggNOGi COG0030.
KOi K02528.
OMAi ILMVQNE.
OrthoDBi EOG66F08Z.

Enzyme and pathway databases

BioCyci MGEN243273:GH2R-516-MONOMER.

Family and domain databases

Gene3Di 1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPi MF_00607. 16SrRNA_methyltr_A.
InterProi IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR11727. PTHR11727. 1 hit.
Pfami PF00398. RrnaAD. 1 hit.
[Graphical view ]
SMARTi SM00650. rADc. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00755. ksgA. 1 hit.
PROSITEi PS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 137-248.
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiRSMA_MYCGE
AccessioniPrimary (citable) accession number: P47701
Secondary accession number(s): Q49194
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3