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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei255ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:MG462
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001196011 – 484Glutamate--tRNA ligaseAdd BLAST484

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243273.MgenG_010200000110.

Structurei

3D structure databases

ProteinModelPortaliP47700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 20"HIGH" regionAdd BLAST11
Motifi252 – 256"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
KOiK01885.
OMAiQLCYMPL.
OrthoDBiPOG091H021W.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47700-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKIRTRYAP SPTGYLHVGG TRTAIFNFLL AKHFNGEFII RIEDTDTERN
60 70 80 90 100
IKEGINSQFD NLRWLGVIAD ESVYNPGNYG PYLQSQKLAV YKKLAFDLIE
110 120 130 140 150
KNLAYRCFCS KEKLESDRKQ AINNHKTPKY LGHCRNLHSK KITNHLEKND
160 170 180 190 200
PFTIRLKINN EAEYSWNDLV RGQITIPGSA LTDIVILKAN GVATYNFAVV
210 220 230 240 250
IDDYDMEITD VLRGAEHISN TAYQLAIYQA LGFKRIPRFG HLSVIVDESG
260 270 280 290 300
KKLSKRDEKT TQFIEQFKQQ GYLPEALLNF LALLGWHPQY NQEFFNLKQL
310 320 330 340 350
IENFSLSRVV SAPAFFDIKK LQWINANYIK QLTDNAYFNF IDNYLDVKVD
360 370 380 390 400
YLKDKNREIS LLFKNQITHG VQINELIRES FATKIGVENL AKKSHILFKN
410 420 430 440 450
IKLFLEQLAK SLQGLEEWKA EQIKTTINKV GAVFNLKGKQ LFMPIRLIFT
460 470 480
NKEHGPDLAH IIEIFDKESA INLIKQFINA TNLF
Length:484
Mass (Da):55,941
Last modified:February 1, 1996 - v1
Checksum:iB1E81B3839A45F6D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60D → EL in AAD12469 (PubMed:8253680).Curated1
Sequence conflicti230A → V (PubMed:1945886).Curated1
Sequence conflicti362L → T (PubMed:8253680).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC72482.1.
X61538 Genomic DNA. Translation: CAA43750.1.
U02183 Genomic DNA. Translation: AAD12469.1.
U02122 Genomic DNA. Translation: AAD12397.1.
PIRiA64251.
RefSeqiWP_009885570.1. NZ_AAGX01000001.1.

Genome annotation databases

EnsemblBacteriaiAAC72482; AAC72482; MG_462.
KEGGimge:MG_462.
PATRICi20010518. VBIMycGen98045_0532.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC72482.1.
X61538 Genomic DNA. Translation: CAA43750.1.
U02183 Genomic DNA. Translation: AAD12469.1.
U02122 Genomic DNA. Translation: AAD12397.1.
PIRiA64251.
RefSeqiWP_009885570.1. NZ_AAGX01000001.1.

3D structure databases

ProteinModelPortaliP47700.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243273.MgenG_010200000110.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC72482; AAC72482; MG_462.
KEGGimge:MG_462.
PATRICi20010518. VBIMycGen98045_0532.

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
KOiK01885.
OMAiQLCYMPL.
OrthoDBiPOG091H021W.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYE_MYCGE
AccessioniPrimary (citable) accession number: P47700
Secondary accession number(s): Q49256, Q49306, Q49490
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.