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P47700 (SYE_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:MG462
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 484484Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000119601

Regions

Motif10 – 2011"HIGH" region HAMAP-Rule MF_00022
Motif252 – 2565"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2551ATP By similarity

Experimental info

Sequence conflict601D → EL in AAD12469. Ref.3
Sequence conflict2301A → V Ref.2
Sequence conflict3621L → T Ref.3

Sequences

Sequence LengthMass (Da)Tools
P47700 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: B1E81B3839A45F6D

FASTA48455,941
        10         20         30         40         50         60 
MEKIRTRYAP SPTGYLHVGG TRTAIFNFLL AKHFNGEFII RIEDTDTERN IKEGINSQFD 

        70         80         90        100        110        120 
NLRWLGVIAD ESVYNPGNYG PYLQSQKLAV YKKLAFDLIE KNLAYRCFCS KEKLESDRKQ 

       130        140        150        160        170        180 
AINNHKTPKY LGHCRNLHSK KITNHLEKND PFTIRLKINN EAEYSWNDLV RGQITIPGSA 

       190        200        210        220        230        240 
LTDIVILKAN GVATYNFAVV IDDYDMEITD VLRGAEHISN TAYQLAIYQA LGFKRIPRFG 

       250        260        270        280        290        300 
HLSVIVDESG KKLSKRDEKT TQFIEQFKQQ GYLPEALLNF LALLGWHPQY NQEFFNLKQL 

       310        320        330        340        350        360 
IENFSLSRVV SAPAFFDIKK LQWINANYIK QLTDNAYFNF IDNYLDVKVD YLKDKNREIS 

       370        380        390        400        410        420 
LLFKNQITHG VQINELIRES FATKIGVENL AKKSHILFKN IKLFLEQLAK SLQGLEEWKA 

       430        440        450        460        470        480 
EQIKTTINKV GAVFNLKGKQ LFMPIRLIFT NKEHGPDLAH IIEIFDKESA INLIKQFINA 


TNLF 

« Hide

References

« Hide 'large scale' references
[1]"The minimal gene complement of Mycoplasma genitalium."
Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. expand/collapse author list , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.
Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]"A random sequencing approach for placing markers on the physical map of Mycoplasma genitalium."
Peterson S.N., Schramm N., Hu P.-C., Bott K.F., Hutchison C.A. III
Nucleic Acids Res. 19:6027-6031(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 188-236.
Strain: ATCC 33530 / G-37 / NCTC 10195.
[3]"A survey of the Mycoplasma genitalium genome by using random sequencing."
Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 29-151 AND 362-484.
Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC72482.1.
X61538 Genomic DNA. Translation: CAA43750.1.
U02183 Genomic DNA. Translation: AAD12469.1.
U02122 Genomic DNA. Translation: AAD12397.1.
PIRA64251.
RefSeqNP_073132.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47700.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_462.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC72482; AAC72482; MG_462.
GeneID875594.
KEGGmge:MG_462.
PATRIC20010518. VBIMycGen98045_0532.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
KOK01885.
OMAAFRCFCT.
OrthoDBEOG6DRPF7.
ProtClustDBPRK01406.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-515-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_MYCGE
AccessionPrimary (citable) accession number: P47700
Secondary accession number(s): Q49256, Q49306, Q49490
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 19, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries