P47668 (PT1_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate-protein phosphotransferase
EC=2.7.3.9

Alternative name(s):
Phosphotransferase system, enzyme I

Gene names

Name:	ptsI
Ordered Locus Names:	MG429

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr) By similarity.
Catalytic activity	Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine.
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Domain	The N-terminal domain contains the HPr binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site By similarity.
Miscellaneous	The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain By similarity.
Sequence similarities	Belongs to the PEP-utilizing enzyme family.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate-protein phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Phosphoenolpyruvate-protein phosphotransferase		PRO_0000147076
Sites
	Active site	190	1	Tele-phosphohistidine intermediate By similarity
	Active site	498	1	Proton donor By similarity
	Metal binding	427	1	Magnesium By similarity
	Metal binding	451	1	Magnesium By similarity
	Binding site	297	1	Substrate By similarity
	Binding site	333	1	Substrate By similarity
	Binding site	427	1	Substrate By similarity
	Binding site	448	1	Substrate; via carbonyl oxygen By similarity
	Binding site	449	1	Substrate; via amide nitrogen By similarity
	Binding site	450	1	Substrate By similarity
	Binding site	451	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47668 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: DFCB4E00589A940E	FASTA	572	64,202
	10 20 30 40 50 60 MKKIIGIGVS DGIAVAKAFI IQTPQFDVKK YTNVKMTPTQ AKKLLSSAFQ KAKKDLEEIK 70 80 90 100 110 120 TITVKNINQE AGMIFDAHIQ ILNDPTITEQ LEQQLNKNIH PVIAVDNVFQ QTALMFSEMD 130 140 150 160 170 180 DKYFKERASD ILDLHQRLLS YLTGVKLNDL IRIKSDVIIV ANDLTPSQTA TLNKKYVKGF 190 200 210 220 230 240 LTESGGKTSH AAIMARSMEI PAIVGLKNIT SKVEDGKTVG INGRKGIVGF DFSSKDITQW 250 260 270 280 290 300 KQEKELESNF QNELKQYTNK LVKTLDGYEV IVASNIGNVK DMDLAVEYNT NGIGLFRTEF 310 320 330 340 350 360 LYMSSQDWPD ESVQFEAYKT VLQKAKNDLV IIRTLDIGGD KKLNYFQFPH EDNPFLGYRA 370 380 390 400 410 420 IRLTLDKQAV FKTQLRALLR ASDYGNLGIM FPMVATLDEL VQVKQLLTKV QQEFNETKKF 430 440 450 460 470 480 KLGIMIEIPS AALAADCLGK HVDFFSIGSN DLIQYSFAAD RMNKNVSYLY QPLNPALLRL 490 500 510 520 530 540 IKLVVEGGKL NNVWTGMCGE MASDQYAIPL LLGLGLTELS MSASSMFKAR MVIAKITINE 550 560 570 CKSLVEKALK LTSDSAVRKL VENFFKKKNI FI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"Novel phosphotransferase system genes revealed by bacterial genome analysis: the complete complement of pts genes in Mycoplasma genitalium." Reizer J., Paulsen I.T., Reizer A., Titgemeyer F., Saier M.H. Jr. Microb. Comp. Genomics 1:151-164(1996) [PubMed: 9689210] [Abstract] Cited for: DISCUSSION OF SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC72450.1.
PIR	D64247.
RefSeq	NP_073100.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47668.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	875624.
GenomeReviews	Gene locus MG429 in contig L43967_GR.
KEGG	mge:MG_429.
PATRIC	20010444. VBIMycGen98045_0495.
TIGR	MG429.
Phylogenomic databases
HOGENOM	HBG456539.
OMA	KITINEC.
PhylomeDB	P47668.
ProtClustDB	CLSK335335.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_429-MONOMER.
Family and domain databases
InterPro	IPR008279. PEP-util_enz_mobile_dom. IPR018274. PEP_util_AS. IPR000121. PEP_util_C. IPR023151. PEP_util_CS. IPR006318. PEP_util_enz. IPR024692. PTS_enz_I. IPR008731. PTS_PEP_utilis_N. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.50.30.10. PEP_mobile. 1 hit. G3DSA:1.10.274.10. PTS_PEP_utilis_N. 1 hit. G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
KO	K08483.
PANTHER	PTHR22931:SF10. PTHR22931:SF10. 1 hit.
Pfam	PF05524. PEP-utilisers_N. 1 hit. PF00391. PEP-utilizers. 1 hit. PF02896. PEP-utilizers_C. 1 hit. [Graphical view]
PIRSF	PIRSF000732. PTS_enzyme_I. 1 hit.
PRINTS	PR01736. PHPHTRNFRASE.
SUPFAM	SSF47831. PEP-utilisers_N. 1 hit. SSF52009. PEP_mobile. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01417. PTS_I_fam. 1 hit.
PROSITE	PS00742. PEP_ENZYMES_2. 1 hit. PS00370. PEP_ENZYMES_PHOS_SITE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PT1_MYCGE

Accession

Primary (citable) accession number: P47668

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program