P47619 (MNMG_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Alternative name(s):
Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	MG379

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP-Rule MF_00129
Cofactor	FAD By similarity.
Subunit structure	Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP-Rule MF_00129		PRO_0000117132
Regions
	Nucleotide binding	9 – 14	6	FAD By similarity
	Nucleotide binding	270 – 284	15	NAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P47619 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 67A3F64C985B5912	FASTA	612	68,995
	10 20 30 40 50 60 MSFIITVIGA GHAGLEAAFI VSKFNIKVNL LVLDINHLGS CPCNPSIGGP AKGIVTREID 70 80 90 100 110 120 VLGGMQAIAA DNNALQYKLL NSSKGPAVQA IRAQIDKIGY KNWFQSQVKL NKNINLIQSE 130 140 150 160 170 180 AINLIVRNEK IKGVILKDGS ELLSDAVIIT TGTYLRSKTY CGNTVKNQGP DQSKNSEKLS 190 200 210 220 230 240 TNLINRGFKT IRLKTGTPPR ILKTSLDYNQ MELEINNNQN LAFSTTNKNF LPLEKQIPCY 250 260 270 280 290 300 LVHTNQKIHD LILKNLKKSA MFNGSISAQG PLYCPSIEDK VFKFSQKPRH QIFVEPESLS 310 320 330 340 350 360 LDTIYLAGLS TSFTPEIQKE IIQLLPGFQN AEIKKFGYAI EYDAFLSNQL KPTLETKLIE 370 380 390 400 410 420 NLYFAGQING TSGYEEAAGQ GLMAGINAAL KLLKKPPFIL QRNEAYIGVM INDLVTKTIS 430 440 450 460 470 480 DPYRLLTSRA EYRLWLRNDN VQERLIKKSF ELGLTDKKTY ELFLKKEKKK QELISFLKNT 490 500 510 520 530 540 QVGKVKALKF TNKNTAQSLY DFNKRSEINL DKLIKDLPEK YQLDSETLKQ IEIEIKYEGY 550 560 570 580 590 600 IKKNEKYFKG LDKLSKIKIP HTFDYHKVKN LASEAIFKLS NFKPSNLAIA SQIAGVNFND 610 IIAIKHFLKT YE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 301-394. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71606.1. U01812 Genomic DNA. Translation: AAD12347.1.
PIR	I64241.
RefSeq	NP_073051.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47619.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_379.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71606; AAC71606; MG_379.
GeneID	875674.
KEGG	mge:MG_379.
PATRIC	20010340. VBIMycGen98045_0444.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0445.
KO	K03495.
OMA	AQMSCNP.
ProtClustDB	PRK05192.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-433-MONOMER.
Family and domain databases
HAMAP	MF_00129. MnmG_GidA.
InterPro	IPR004416. GidA. IPR026904. GidA-assoc_3. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
Pfam	PF01134. GIDA. 1 hit. PF13932. GIDA_assoc_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00136. gidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNMG_MYCGE

Accession

Primary (citable) accession number: P47619

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program