P47607 (RNC_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	MG367

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_0000180410
Regions
	Domain	18 – 141	124	RNase III
Sites
	Active site	63	1	Potential
	Active site	130	1	By similarity
	Metal binding	59	1	Magnesium By similarity
	Metal binding	127	1	Magnesium By similarity
	Metal binding	130	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47607 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: B54D2A893EA01CBE	FASTA	262	30,338
	10 20 30 40 50 60 MKNKVLKLKN NKIFDKKLAT FLKNLDIFPN NWEFFEKAFI HASYINEHED VSESYDRLEF 70 80 90 100 110 120 LGDALIDFVV AKKLFELYPK YNEGLLTRTK IEIVKGENLN RIGMELKLGD FVKLSNGAEL 130 140 150 160 170 180 TENTVGDVLE ALVGAIYEDM GMKKATEFVE KYIFERTFSE ILKYDFFSLF QEQKLPEPRV 190 200 210 220 230 240 RVSLTSNNLV LSIIELDGDI IWSQAIPNNK NYDDKSVLEH NAMASFTSFL KSSKGSHFFS 250 260 DLKEKIENQK MCKKLAIKPK KN

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71594.1.
PIR	F64240.
RefSeq	NP_073039.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47607.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_367.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71594; AAC71594; MG_367.
GeneID	875426.
KEGG	mge:MG_367.
PATRIC	20010316. VBIMycGen98045_0432.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0571.
KO	K03685.
OMA	VLEHNAM.
ProtClustDB	CLSK335305.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-421-MONOMER.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_MYCGE

Accession

Primary (citable) accession number: P47607

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program