P47605 (FMT_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	MG365

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182
Sequence similarities	Belongs to the fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182		PRO_0000082993
Regions
	Region	109 – 112	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47605 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 38D96D99DCDC1EE0	FASTA	311	35,536
	10 20 30 40 50 60 MFKIVFFGTS TLSKKCLEQL FYDNDFEICA VVTQPDKINH RNNKIVPSDV KSFCLEKNIT 70 80 90 100 110 120 FFQPKQSISI KADLEKLKAD IGICVSFGQY LHQDIIDLFP NKVINLHPSK LPLLRGGAPL 130 140 150 160 170 180 HWTIINGFKK SALSVIQLVK KMDAGPIWKQ QDFLVNNDWN TGDLSIYVEE HSPSFLIECT 190 200 210 220 230 240 KEILNKKGKW FEQIGEPTFG LNIRKEQEHL DLNQIYKSFL NWVKGLAPKP GGWLSFEGKN 250 260 270 280 290 300 IKIFKAKYVS KSNYKHQLGE IVNISRKGIN IALKSNEIIS IEKIQIPGKR VMEVSEIING 310 KHPFVVGKCF K

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71592.1.
PIR	D64240.
RefSeq	NP_073037.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47605.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_365.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71592; AAC71592; MG_365.
GeneID	875424.
KEGG	mge:MG_365.
PATRIC	20010312. VBIMycGen98045_0430.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
KO	K00604.
OMA	PEKANEE.
ProtClustDB	PRK00005.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-419-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_MYCGE

Accession

Primary (citable) accession number: P47605

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program