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Protein

Acetate kinase

Gene

ackA

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg2+. Can also accept Mn2+.UniRule annotation

Pathwayi: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. Phosphate acetyltransferase (pta)
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10MagnesiumUniRule annotation1
Binding sitei17ATPUniRule annotation1
Binding sitei89SubstrateUniRule annotation1
Active sitei146Proton donor/acceptorUniRule annotation1
Sitei178Transition state stabilizerUniRule annotation1
Sitei237Transition state stabilizerUniRule annotation1
Metal bindingi376MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi204 – 208ATPUniRule annotation5
Nucleotide bindingi278 – 280ATPUniRule annotation3
Nucleotide bindingi323 – 327ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Ordered Locus Names:MG357
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001075861 – 393Acetate kinaseAdd BLAST393

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243273.MgenG_010200001928.

Structurei

3D structure databases

ProteinModelPortaliP47599.
SMRiP47599.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6H. Bacteria.
COG0282. LUCA.
KOiK00925.
OMAiWKALTGT.
OrthoDBiPOG091H02K4.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase. 1 hit.
InterProiView protein in InterPro
IPR004372. Ac/propionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiView protein in Pfam
PF00871. Acetate_kinase. 1 hit.
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiView protein in PROSITE
PS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P47599-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSHKILVVN AGSSSIKFQL FNDKKQVLAK GLCERIFIDG FFKLEFNQKK
60 70 80 90 100
IEEKVQFNDH NLAVKHFLNA LKKNKIITEL SEIGLIGHRV VQGANYFTDA
110 120 130 140 150
VLVDTHSLAK IKEFIKLAPL HNKPEADVIE IFLKEIKTAK NVAVFDTTFH
160 170 180 190 200
TTIPRENYLY AVPENWEKNN LVRRYGFHGT SYKYINEFLE KKFNKKPLNL
210 220 230 240 250
IVCHLGNGAS VCAIKQGKSL NTSMGFTPLE GLIMGTRSGD IDPAIVSYIA
260 270 280 290 300
EQQKLSCNDV VNELNKKSGM FAITGSSDMR DIFDKPEIND IAIKMYVNRV
310 320 330 340 350
ADYIAKYLNQ LSGEIDSLVF TGGVGENASY CVQLIIEKVA SLGFKTNSNL
360 370 380 390
FGNYQDSSLI STNESKYQIF RVRTNEELMI VEDALRVSTN IKK
Length:393
Mass (Da):44,346
Last modified:February 1, 1996 - v1
Checksum:i985CE65FC7B92225
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48Q → S in CAA43743 (PubMed:1945886).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71582.1.
X61531 Genomic DNA. Translation: CAA43743.1.
PIRiE64239.
RefSeqiWP_009885814.1. NZ_AAGX01000006.1.

Genome annotation databases

EnsemblBacteriaiAAC71582; AAC71582; MG_357.
KEGGimge:MG_357.

Similar proteinsi

Entry informationi

Entry nameiACKA_MYCGE
AccessioniPrimary (citable) accession number: P47599
Secondary accession number(s): Q49485
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 7, 2017
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families