P47593 (IPYR_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	MG351

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP-Rule MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00209.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 184	184	Inorganic pyrophosphatase HAMAP-Rule MF_00209		PRO_0000137507
Sites
	Metal binding	55	1	Magnesium 1 By similarity
	Metal binding	60	1	Magnesium 1 By similarity
	Metal binding	60	1	Magnesium 2 By similarity
	Metal binding	92	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47593 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 3A1F1006362FE3B9	FASTA	184	21,646
	10 20 30 40 50 60 MDKFLIDVIV EIPKNSKIKY EYDRQTGQIR VDRILFGSES YPQNYGFIKN TLDWDGDELD 70 80 90 100 110 120 CFIFADQPFL PATVVPTRIV GALEMIDDGE IDTKLLGVID CDPRYKEINQ ISDLPKHRIE 130 140 150 160 170 180 EILIFLKTYK LLQKKTVIIK GLKDVCWAKK EYEICLQLMK DYGHLSKDQF IQKMQILHPE HYQK

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71576.1.
PIR	H64238.
RefSeq	NP_073022.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47593.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_351.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71576; AAC71576; MG_351.
GeneID	875279.
KEGG	mge:MG_351.
PATRIC	20010278. VBIMycGen98045_0413.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0221.
KO	K01507.
OMA	KGEDEKI.
ProtClustDB	PRK02230.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-405-MONOMER.
Family and domain databases
Gene3D	3.90.80.10. 1 hit.
HAMAP	MF_00209. Inorganic_PPase.
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
PANTHER	PTHR10286. PTHR10286. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_MYCGE

Accession

Primary (citable) accession number: P47593

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program