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P47589 (TRMB_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:MG347
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171351

Regions

Region188 – 1914Substrate binding By similarity

Sites

Active site1121 By similarity
Binding site361S-adenosyl-L-methionine By similarity
Binding site611S-adenosyl-L-methionine By similarity
Binding site901S-adenosyl-L-methionine By similarity
Binding site1121S-adenosyl-L-methionine By similarity
Binding site1161Substrate By similarity
Binding site1481Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P47589 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 5F9B0DCA113ABB26

FASTA21024,929
        10         20         30         40         50         60 
MRLRKVKNAL LKINQSPYFY SKDKFAKFTK KQLVLELGCG KGTFLIKEAQ KNNNFLFIGI 

        70         80         90        100        110        120 
EREPTIVLKA INKINKLDFN LENILLLCTD AKQLDDYFQA ESVQKIFINF PDPWPKKRHI 

       130        140        150        160        170        180 
QRRLTSPDFL KLFWNLLVKN GLIEFKTDND KLFEYTLTTL QENSQIFEII HQITDLNNSE 

       190        200        210 
FSFQNSITEY EQRFMELEIP IKKLVIKKII 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC71572.1.
PIRD64238.
RefSeqNP_073017.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47589.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_347.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC71572; AAC71572; MG_347.
GeneID875414.
KEGGmge:MG_347.
PATRIC20010260. VBIMycGen98045_0407.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMAKTDDDEL.
OrthoDBEOG6K6VBC.
ProtClustDBCLSK335295.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-397-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_MYCGE
AccessionPrimary (citable) accession number: P47589
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names