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P47587

- SYI_MYCGE

UniProt

P47587 - SYI_MYCGE

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei549 – 5491Aminoacyl-adenylateUniRule annotation
Binding sitei593 – 5931ATPUniRule annotation
Metal bindingi869 – 8691ZincUniRule annotation
Metal bindingi872 – 8721ZincUniRule annotation
Metal bindingi888 – 8881ZincUniRule annotation
Metal bindingi891 – 8911ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-395-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MG345
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 895895Isoleucine--tRNA ligasePRO_0000098417Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243273.MG_345.

Structurei

3D structure databases

ProteinModelPortaliP47587.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi590 – 5945"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47587-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDLKKTLLMP KTSFAMQANL STSEKNFHDF WKDKKVFQKL KKQNKGKQIK
60 70 80 90 100
ILHDGPPYAN GSIHVGHALN KILKDFILRS WLYEGYDVVF IPGWDCHGLP
110 120 130 140 150
IEHAVSKKNP SSYSNLSTVE KRKLCHQFAL SQIAVQKEQF QRLGLLNDFQ
160 170 180 190 200
NCYYTIDESF QFKELELFLQ AIKKGLIFQD LKPTYWSPIS RTSLAEAEIE
210 220 230 240 250
YKEVNSIALY LTFKVSKSDF LDENANLLVW TTTPWTLPTN QAIAIHPDFD
260 270 280 290 300
YLLFEYNQQK FVILEKLFEV FTNKLNWTNA IKLKKFKGSN LKNSSYSHCF
310 320 330 340 350
YNKVLPVLMG IHVVDNEGTG IVHSSPAFGI DDFYLCQKNK IKEVLISIDE
360 370 380 390 400
KGVFNNLLND KELENCFYLK ANDLIINRLK QNNSFIFSEV ISHREPHDWR
410 420 430 440 450
SKTPVIYRAS KQLFIKTKSI KKQLKKQINQ VNFLNSKNQL RLKEMLLQRD
460 470 480 490 500
EWCISRQRVW GLPIPIVYAN NKPLLDFSTI QYTIKQLKKH GIDSWFEKDV
510 520 530 540 550
TCFLKPDKTK KWVKYHKEID TLDVWFDSGS SYNVLEINKY GSIADLYIEG
560 570 580 590 600
SDQYRGWFNS SSNCGIIQND LIPFKSLVSH GFTLDENGNK MSKSLGNIVD
610 620 630 640 650
PLKICDQYGA DILRLWVANT DWQIDNKIGV NILKQVAEQY RRIRNSLLRF
660 670 680 690 700
ILGNINGFNF TSMDDYKFSL EDKIVIHKTN SLVEQIEKFL EKYNFLGCLK
710 720 730 740 750
VINKFVLWLS SWYFEIIKDT LYCDAKNNPN RLAKQAVLNY IFTQLISFLN
760 770 780 790 800
IFIPHTAEDA WKNYSFNKKP ISVNLFTKPT VFKVANSKNL GNIYKTFTSI
810 820 830 840 850
KNAAFKEIEK LRKEGLISKN NQIELTVGIN KKIPKKLKDN LALWLNVNSV
860 870 880 890
NLTNNENEIK VKKTKKTMCE RCWNFQTIIK QKLDHNLCSR CFKVC
Length:895
Mass (Da):104,396
Last modified:February 1, 1996 - v1
Checksum:i8C78DE6A05311B22
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71570.1.
U02196 Genomic DNA. Translation: AAD12482.1.
U02254 Genomic DNA. Translation: AAD12519.1.
PIRiB64238.
RefSeqiNP_073015.2. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71570; AAC71570; MG_345.
GeneIDi875496.
KEGGimge:MG_345.
PATRICi20010256. VBIMycGen98045_0405.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71570.1 .
U02196 Genomic DNA. Translation: AAD12482.1 .
U02254 Genomic DNA. Translation: AAD12519.1 .
PIRi B64238.
RefSeqi NP_073015.2. NC_000908.2.

3D structure databases

ProteinModelPortali P47587.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243273.MG_345.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC71570 ; AAC71570 ; MG_345 .
GeneIDi 875496.
KEGGi mge:MG_345.
PATRICi 20010256. VBIMycGen98045_0405.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MGEN243273:GH2R-395-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 262-371 AND 605-711.
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiSYI_MYCGE
AccessioniPrimary (citable) accession number: P47587
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3