P47582 (RPOC_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta'
Short name=RNAP subunit beta'
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta'
Transcriptase subunit beta'

Gene names

Name:	rpoC
Ordered Locus Names:	MG340

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	1292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP-Rule MF_01322
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_01322
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP
Molecular_function	DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1292	1292	DNA-directed RNA polymerase subunit beta' HAMAP-Rule MF_01322		PRO_0000067759
Experimental info
	Sequence conflict	33 – 36	4	WSEG → LIWR in AAD12323. Ref.2
	Sequence conflict	338 – 344	7	NDRLRRI → KWPIKKD Ref.3
	Sequence conflict	1029 – 1037	9	LTMRTFHTG → MDNAYFPYW Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47582 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 15003759D34D17A5	FASTA	1,292	145,659
	10 20 30 40 50 60 MTTTRRNKRN NKLYKNIKAI KLSIASNDTI LNWSEGEVTK AETINYKSLK PEPGGLFDEA 70 80 90 100 110 120 IFGPVKDYEC ACGKFKKIKY RGVRCDRCGV WVTESIVRRE RMGHIALVSP VAHIWMSKEL 130 140 150 160 170 180 PSPSKISLVL NISYKEVEQV LYFVNYIVLD TGKIKDDKIM PFKFKEVLDL TGKGSLSTRQ 190 200 210 220 230 240 KMRRVIGYIF RNLIKSKSSE DYRKGKIFYE SLKNSSLPFS LNDAFNYIKK YTGFRVGIGA 250 260 270 280 290 300 EAILELLNKI DLNLEFSRLN DALRKAKKDS VEDAKVKKIL RQLETISWFR NSKLHPKNMI 310 320 330 340 350 360 LHTVPVIPPD IRPIIQLDGA KFTTSDINNF YRRVIIRNDR LRRILEDGTV PSIVVNNEKR 370 380 390 400 410 420 LLQESVDALF DNSSRHKPSL SKDKRSLKSL TDRLKGKQGL FRHNLLGKRV DYSGRSVIVV 430 440 450 460 470 480 GPELKMYEVG IPALMILKLF KPFIIHGLIN KFDENGNEIR PIAASIRQAE DMIKNQDDLI 490 500 510 520 530 540 WGIVYDVIKD RPVLLNRAPT LHRLGIQAFE PRIVDGKAIR LHPLVTTAFN ADFDGDQMAV 550 560 570 580 590 600 HVPLSENAVN EARAVLLASK HILGLKDGRP IVTPTQDMVL GNYYLTTERK GQLGEGIIFS 610 620 630 640 650 660 TVYEARAAYE SQKVHLHAIV GISTKAFPNK KFACQGTLIT TVGKIIFNDV LGNNVPYIND 670 680 690 700 710 720 GEFDENACPE KFIVKQGEDV RQSILKHQII PAFSKKVISK LIDLLYLLLE FKDLPKTLDN 730 740 750 760 770 780 IKALGFKYST FSSTTVSVFD IPKYTNKQNY FDSADQQVLK YKQFYNKGLL TDDERYKRVV 790 800 810 820 830 840 KLWNNVKEKV SDEIQNLIKQ EQYRDNSIVV MADSGARGNI SNFTQLFGMR GLMSKSFNYE 850 860 870 880 890 900 RNNQSKIIKD TIEVPIKHSF FEGLTINEYF NSSYGARKGM TDTAMKTAKS GYMTRKLVDA 910 920 930 940 950 960 THELIINHDD CGTRKGIVVE AIVETKTKSL IESLFDRIVN RYSITPIVDP ETQKTIVEAN 970 980 990 1000 1010 1020 SLITTQLAKQ ICATSIKEVL VRSVIYCERE NGICQYCFGI DLSTGKLVEL GTAVGVIAAQ 1030 1040 1050 1060 1070 1080 SIGEPGTQLT MRTFHTGGVS TENNLAQGFE RLKQIFEVVT PKDFEKAVIS EVKGTVKSIT 1090 1100 1110 1120 1130 1140 TVQNAQEVVI KSNVDERIYT IPFSAQIRVH VGDQVSPGSK ITEGSVDIKQ LLRIAGIQRV 1150 1160 1170 1180 1190 1200 RQYMIVEIQK VYRIQGIDIA DKYVEIIIRQ LTNLLQVTDA GNSNLFVGQL VHSHYLNELN 1210 1220 1230 1240 1250 1260 KSLLLAGKMP VIAINQVFGI DEAASKSNSF LSAASFQDTK KILTDAAVKN QVDYLLGLKE 1270 1280 1290 NVIIGGKIPA GTGFLTDEEL TFLGSKTVAE EY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 23-142 AND 1029-1148. Strain: ATCC 33530 / G-37 / NCTC 10195.
[3]	"A random sequencing approach for placing markers on the physical map of Mycoplasma genitalium." Peterson S.N., Schramm N., Hu P.-C., Bott K.F., Hutchison C.A. III Nucleic Acids Res. 19:6027-6031(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 338-506. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71565.1. U01797 Genomic DNA. Translation: AAD12323.1. U02169 Genomic DNA. Translation: AAD12451.1. X61528 Genomic DNA. Translation: CAB98132.1. X61534 Genomic DNA. Translation: CAA43746.1. Sequence problems.
PIR	F64237.
RefSeq	NP_073010.2. NC_000908.2.
3D structure databases
ProteinModelPortal	P47582.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_340.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71565; AAC71565; MG_340.
GeneID	875481.
KEGG	mge:MG_340.
PATRIC	20010246. VBIMycGen98045_0400.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0086.
KO	K03046.
ProtClustDB	PRK00566.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-390-MONOMER.
Family and domain databases
Gene3D	2.40.40.20. 2 hits.
HAMAP	MF_01322. RNApol_bact_RpoC.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR012754. DNA-dir_RpoC_beta_prime. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02386. rpoC_TIGR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOC_MYCGE

Accession

Primary (citable) accession number: P47582
Secondary accession number(s): Q49292 , Q49454, Q49483, Q49486

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program