Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P47582 (RPOC_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit beta'

Short name=RNAP subunit beta'
EC=2.7.7.6
Alternative name(s):
RNA polymerase subunit beta'
Transcriptase subunit beta'
Gene names
Name:rpoC
Ordered Locus Names:MG340
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length1292 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP-Rule MF_01322

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_01322

Subunit structure

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.

Sequence similarities

Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12921292DNA-directed RNA polymerase subunit beta' HAMAP-Rule MF_01322
PRO_0000067759

Experimental info

Sequence conflict33 – 364WSEG → LIWR in AAD12323. Ref.2
Sequence conflict338 – 3447NDRLRRI → KWPIKKD Ref.3
Sequence conflict1029 – 10379LTMRTFHTG → MDNAYFPYW Ref.2

Sequences

Sequence LengthMass (Da)Tools
P47582 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 15003759D34D17A5

FASTA1,292145,659
        10         20         30         40         50         60 
MTTTRRNKRN NKLYKNIKAI KLSIASNDTI LNWSEGEVTK AETINYKSLK PEPGGLFDEA 

        70         80         90        100        110        120 
IFGPVKDYEC ACGKFKKIKY RGVRCDRCGV WVTESIVRRE RMGHIALVSP VAHIWMSKEL 

       130        140        150        160        170        180 
PSPSKISLVL NISYKEVEQV LYFVNYIVLD TGKIKDDKIM PFKFKEVLDL TGKGSLSTRQ 

       190        200        210        220        230        240 
KMRRVIGYIF RNLIKSKSSE DYRKGKIFYE SLKNSSLPFS LNDAFNYIKK YTGFRVGIGA 

       250        260        270        280        290        300 
EAILELLNKI DLNLEFSRLN DALRKAKKDS VEDAKVKKIL RQLETISWFR NSKLHPKNMI 

       310        320        330        340        350        360 
LHTVPVIPPD IRPIIQLDGA KFTTSDINNF YRRVIIRNDR LRRILEDGTV PSIVVNNEKR 

       370        380        390        400        410        420 
LLQESVDALF DNSSRHKPSL SKDKRSLKSL TDRLKGKQGL FRHNLLGKRV DYSGRSVIVV 

       430        440        450        460        470        480 
GPELKMYEVG IPALMILKLF KPFIIHGLIN KFDENGNEIR PIAASIRQAE DMIKNQDDLI 

       490        500        510        520        530        540 
WGIVYDVIKD RPVLLNRAPT LHRLGIQAFE PRIVDGKAIR LHPLVTTAFN ADFDGDQMAV 

       550        560        570        580        590        600 
HVPLSENAVN EARAVLLASK HILGLKDGRP IVTPTQDMVL GNYYLTTERK GQLGEGIIFS 

       610        620        630        640        650        660 
TVYEARAAYE SQKVHLHAIV GISTKAFPNK KFACQGTLIT TVGKIIFNDV LGNNVPYIND 

       670        680        690        700        710        720 
GEFDENACPE KFIVKQGEDV RQSILKHQII PAFSKKVISK LIDLLYLLLE FKDLPKTLDN 

       730        740        750        760        770        780 
IKALGFKYST FSSTTVSVFD IPKYTNKQNY FDSADQQVLK YKQFYNKGLL TDDERYKRVV 

       790        800        810        820        830        840 
KLWNNVKEKV SDEIQNLIKQ EQYRDNSIVV MADSGARGNI SNFTQLFGMR GLMSKSFNYE 

       850        860        870        880        890        900 
RNNQSKIIKD TIEVPIKHSF FEGLTINEYF NSSYGARKGM TDTAMKTAKS GYMTRKLVDA 

       910        920        930        940        950        960 
THELIINHDD CGTRKGIVVE AIVETKTKSL IESLFDRIVN RYSITPIVDP ETQKTIVEAN 

       970        980        990       1000       1010       1020 
SLITTQLAKQ ICATSIKEVL VRSVIYCERE NGICQYCFGI DLSTGKLVEL GTAVGVIAAQ 

      1030       1040       1050       1060       1070       1080 
SIGEPGTQLT MRTFHTGGVS TENNLAQGFE RLKQIFEVVT PKDFEKAVIS EVKGTVKSIT 

      1090       1100       1110       1120       1130       1140 
TVQNAQEVVI KSNVDERIYT IPFSAQIRVH VGDQVSPGSK ITEGSVDIKQ LLRIAGIQRV 

      1150       1160       1170       1180       1190       1200 
RQYMIVEIQK VYRIQGIDIA DKYVEIIIRQ LTNLLQVTDA GNSNLFVGQL VHSHYLNELN 

      1210       1220       1230       1240       1250       1260 
KSLLLAGKMP VIAINQVFGI DEAASKSNSF LSAASFQDTK KILTDAAVKN QVDYLLGLKE 

      1270       1280       1290 
NVIIGGKIPA GTGFLTDEEL TFLGSKTVAE EY 

« Hide

References

« Hide 'large scale' references
[1]"The minimal gene complement of Mycoplasma genitalium."
Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. expand/collapse author list , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.
Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]"A survey of the Mycoplasma genitalium genome by using random sequencing."
Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 23-142 AND 1029-1148.
Strain: ATCC 33530 / G-37 / NCTC 10195.
[3]"A random sequencing approach for placing markers on the physical map of Mycoplasma genitalium."
Peterson S.N., Schramm N., Hu P.-C., Bott K.F., Hutchison C.A. III
Nucleic Acids Res. 19:6027-6031(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 338-506.
Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC71565.1.
U01797 Genomic DNA. Translation: AAD12323.1.
U02169 Genomic DNA. Translation: AAD12451.1.
X61528 Genomic DNA. Translation: CAB98132.1.
X61534 Genomic DNA. Translation: CAA43746.1. Sequence problems.
PIRF64237.
RefSeqNP_073010.2. NC_000908.2.

3D structure databases

ProteinModelPortalP47582.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_340.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC71565; AAC71565; MG_340.
GeneID875481.
KEGGmge:MG_340.
PATRIC20010246. VBIMycGen98045_0400.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0086.
KOK03046.
OrthoDBEOG6M9DS6.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-390-MONOMER.

Family and domain databases

HAMAPMF_01322. RNApol_bact_RpoC.
InterProIPR012754. DNA-dir_RpoC_beta_prime.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PfamPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTSM00663. RPOLA_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR02386. rpoC_TIGR. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRPOC_MYCGE
AccessionPrimary (citable) accession number: P47582
Secondary accession number(s): Q49292 expand/collapse secondary AC list , Q49454, Q49483, Q49486
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names