UniProtKB - P47576 (SYV_MYCGE)
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Protein
Valine--tRNA ligase
Gene
valS
Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Functioni
Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.UniRule annotation
Catalytic activityi
ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 517 | ATPUniRule annotation | 1 |
GO - Molecular functioni
- aminoacyl-tRNA editing activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- valine-tRNA ligase activity Source: UniProtKB-EC
GO - Biological processi
- valyl-tRNA aminoacylation Source: InterPro
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MGEN243273:G1GJ2-416-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Valine--tRNA ligaseUniRule annotation (EC:6.1.1.9UniRule annotation)Alternative name(s): Valyl-tRNA synthetaseUniRule annotation Short name: ValRSUniRule annotation |
Gene namesi | Name:valSUniRule annotation Ordered Locus Names:MG334 |
Organismi | Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) |
Taxonomic identifieri | 243273 [NCBI] |
Taxonomic lineagei | Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000106230 | 1 – 837 | Valine--tRNA ligaseAdd BLAST | 837 |
Interactioni
Subunit structurei
Monomer.UniRule annotation
Protein-protein interaction databases
STRINGi | 243273.MgenG_010200003096 |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 767 – 837 | UniRule annotationAdd BLAST | 71 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 46 – 56 | "HIGH" regionAdd BLAST | 11 | |
Motifi | 514 – 518 | "KMSKS" region | 5 |
Domaini
ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.UniRule annotation
The C-terminal coiled-coil domain is crucial for aminoacylation activity.UniRule annotation
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG4105CA4 Bacteria COG0525 LUCA |
KOi | K01873 |
OMAi | RQWYIRN |
OrthoDBi | POG091H01HY |
Family and domain databases
CDDi | cd07962 Anticodon_Ia_Val, 1 hit |
Gene3Di | 1.10.287.3801 hit 3.40.50.6202 hits 3.90.740.101 hit |
HAMAPi | MF_02004 Val_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412 aa-tRNA-synth_I_CS IPR002300 aa-tRNA-synth_Ia IPR033705 Anticodon_Ia_Val IPR013155 M/V/L/I-tRNA-synth_anticd-bd IPR014729 Rossmann-like_a/b/a_fold IPR010978 tRNA-bd_arm IPR009080 tRNAsynth_Ia_anticodon-bd IPR037118 Val-tRNA_synth_C_sf IPR019499 Val-tRNA_synth_tRNA-bd IPR009008 Val/Leu/Ile-tRNA-synth_edit IPR002303 Valyl-tRNA_ligase |
Pfami | View protein in Pfam PF08264 Anticodon_1, 1 hit PF00133 tRNA-synt_1, 1 hit PF10458 Val_tRNA-synt_C, 1 hit |
PRINTSi | PR00986 TRNASYNTHVAL |
SUPFAMi | SSF46589 SSF46589, 1 hit SSF47323 SSF47323, 1 hit SSF50677 SSF50677, 1 hit |
TIGRFAMsi | TIGR00422 valS, 1 hit |
PROSITEi | View protein in PROSITE PS00178 AA_TRNA_LIGASE_I, 1 hit |
i Sequence
Sequence statusi: Complete.
P47576-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKDKFSFQKN YDFNLVSDGL YEIWNNAGFF KPKDKNNSFT AILPPPNLTG
60 70 80 90 100
TLHIGHAFEV SITDQIMRFK KMQGFSINWI PGFDHAGIAT QTKYEKIALK
110 120 130 140 150
ENQKYFDADD DKKSEMIMNW ALNQSEIIKN QLKSLGVCLN WSETKFTLSE
160 170 180 190 200
QANKIVNNCF KNLYENGFIY QAYTLVNWDT KLNTAISNIE VINKPVNQHL
210 220 230 240 250
HYVVYKLAND SKQELIVATT RPETIFADVC LLVNPKDKRY TNFWNKLVVN
260 270 280 290 300
PLTGKQIPVV TDSYVDIKFG TGILKCTPAH DFNDYEINTK YKFDFLSCID
310 320 330 340 350
SNGILNQNAS KFQGLSVLQA RNKIVKWLEK NKLLVKSIPL TSNVGFSERS
360 370 380 390 400
GTVVEPMLSK QWFVDLPKLK DHLYLKKYPD FIPKRFNKQV SNWLNKLKPW
410 420 430 440 450
CISRQLIWGH KIPVWFENNT GEIVVGEKPS KNLQNYTRSK DVLDTWFSSS
460 470 480 490 500
LWPLICLNWE QDDSFHETEL LVTGYDILFF WVLRMLFNSF FETKKLPFKT
510 520 530 540 550
VLIHGLVRDE QNRKMSKSLN NGIDPVDLIR NYGADAVRLF LCSNHTPGDD
560 570 580 590 600
LIFSEQKIKS AWNFLNKLWN VTKFVIQLEN DQEISYDLDK LSLSETWILA
610 620 630 640 650
KLDKVIQKIT KLLDKFQLAL ANQILVKFVW DDFCNTFIEA IKKEPNQLKP
660 670 680 690 700
QLFYTAKSVL SNIAILLSIT VPFLSERIYQ QFNNKSVMQA TWPLATKIKI
710 720 730 740 750
PKLFDLVLAA INDLRNYRKQ YMLNSQQKLV VILSGKNAVD VKQYFNFSWI
760 770 780 790 800
ELKIETNKKV SFKYQIVDDT TQRLKSLQKQ QAFFESEVKR SQAIVKNKSF
810 820 830
LEKAPKEKVK SEFLKLEEYQ KKLTETNQLI AKLTKAH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L43967 Genomic DNA Translation: AAC71557.1 U02249 Genomic DNA Translation: AAD12537.1 |
PIRi | I64236 |
RefSeqi | WP_010869437.1, NC_000908.2 |
Genome annotation databases
EnsemblBacteriai | AAC71557; AAC71557; MG_334 |
KEGGi | mge:MG_334 |
Similar proteinsi
Entry informationi
Entry namei | SYV_MYCGE | |
Accessioni | P47576Primary (citable) accession number: P47576 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | February 28, 2018 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |