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UniProtKB - P47576 (SYV_MYCGE)

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Protein

Valine--tRNA ligase

Gene

valS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.UniRule annotation

Catalytic activityi

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei517ATPUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Valine--tRNA ligaseUniRule annotation (EC:6.1.1.9UniRule annotation)
Alternative name(s):
Valyl-tRNA synthetaseUniRule annotation
Short name:
ValRSUniRule annotation
Gene namesi
Name:valSUniRule annotation
Ordered Locus Names:MG334
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma ›
Proteomesi
  • UP000000807 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001062301 – 837Valine--tRNA ligaseAdd BLAST837

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243273.MgenG_010200003096.

Structurei

3D structure databases

ProteinModelPortaliP47576.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili767 – 837UniRule annotationAdd BLAST71

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi46 – 56"HIGH" regionAdd BLAST11
Motifi514 – 518"KMSKS" region5

Domaini

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.UniRule annotation
The C-terminal coiled-coil domain is crucial for aminoacylation activity.UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105CA4. Bacteria.
COG0525. LUCA.
KOiK01873.
OMAiRQWYIRN.
OrthoDBiPOG091H01HY.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.287.380. 1 hit.
1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_02004. Val_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
PfamiView protein in Pfam
PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF10458. Val_tRNA-synt_C. 1 hit.
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF46589. SSF46589. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

P47576-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDKFSFQKN YDFNLVSDGL YEIWNNAGFF KPKDKNNSFT AILPPPNLTG 
        60         70         80         90        100
TLHIGHAFEV SITDQIMRFK KMQGFSINWI PGFDHAGIAT QTKYEKIALK 
       110        120        130        140        150
ENQKYFDADD DKKSEMIMNW ALNQSEIIKN QLKSLGVCLN WSETKFTLSE 
       160        170        180        190        200
QANKIVNNCF KNLYENGFIY QAYTLVNWDT KLNTAISNIE VINKPVNQHL 
       210        220        230        240        250
HYVVYKLAND SKQELIVATT RPETIFADVC LLVNPKDKRY TNFWNKLVVN 
       260        270        280        290        300
PLTGKQIPVV TDSYVDIKFG TGILKCTPAH DFNDYEINTK YKFDFLSCID 
       310        320        330        340        350
SNGILNQNAS KFQGLSVLQA RNKIVKWLEK NKLLVKSIPL TSNVGFSERS 
       360        370        380        390        400
GTVVEPMLSK QWFVDLPKLK DHLYLKKYPD FIPKRFNKQV SNWLNKLKPW 
       410        420        430        440        450
CISRQLIWGH KIPVWFENNT GEIVVGEKPS KNLQNYTRSK DVLDTWFSSS 
       460        470        480        490        500
LWPLICLNWE QDDSFHETEL LVTGYDILFF WVLRMLFNSF FETKKLPFKT 
       510        520        530        540        550
VLIHGLVRDE QNRKMSKSLN NGIDPVDLIR NYGADAVRLF LCSNHTPGDD 
       560        570        580        590        600
LIFSEQKIKS AWNFLNKLWN VTKFVIQLEN DQEISYDLDK LSLSETWILA 
       610        620        630        640        650
KLDKVIQKIT KLLDKFQLAL ANQILVKFVW DDFCNTFIEA IKKEPNQLKP 
       660        670        680        690        700
QLFYTAKSVL SNIAILLSIT VPFLSERIYQ QFNNKSVMQA TWPLATKIKI 
       710        720        730        740        750
PKLFDLVLAA INDLRNYRKQ YMLNSQQKLV VILSGKNAVD VKQYFNFSWI 
       760        770        780        790        800
ELKIETNKKV SFKYQIVDDT TQRLKSLQKQ QAFFESEVKR SQAIVKNKSF 
       810        820        830 
LEKAPKEKVK SEFLKLEEYQ KKLTETNQLI AKLTKAH
Length:837
Mass (Da):97,464
Last modified:February 1, 1996 - v1
Checksum:i7F2FD1FE95D3D8C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71557.1.
U02249 Genomic DNA. Translation: AAD12537.1.
PIRiI64236.
RefSeqiWP_010869437.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71557; AAC71557; MG_334.
KEGGimge:MG_334.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71557.1.
U02249 Genomic DNA. Translation: AAD12537.1.
PIRiI64236.
RefSeqiWP_010869437.1. NC_000908.2.

3D structure databases

ProteinModelPortaliP47576.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243273.MgenG_010200003096.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71557; AAC71557; MG_334.
KEGGimge:MG_334.

Phylogenomic databases

eggNOGiENOG4105CA4. Bacteria.
COG0525. LUCA.
KOiK01873.
OMAiRQWYIRN.
OrthoDBiPOG091H01HY.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.287.380. 1 hit.
1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_02004. Val_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
PfamiView protein in Pfam
PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF10458. Val_tRNA-synt_C. 1 hit.
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF46589. SSF46589. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYV_MYCGE
AccessioniPrimary (citable) accession number: P47576
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 7, 2017
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.