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P47576

- SYV_MYCGE

UniProt

P47576 - SYV_MYCGE

Protein

Valine--tRNA ligase

Gene

valS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.UniRule annotation

    Catalytic activityi

    ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei517 – 5171ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. valine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. valyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMGEN243273:GH2R-382-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Valine--tRNA ligaseUniRule annotation (EC:6.1.1.9UniRule annotation)
    Alternative name(s):
    Valyl-tRNA synthetaseUniRule annotation
    Short name:
    ValRSUniRule annotation
    Gene namesi
    Name:valSUniRule annotation
    Ordered Locus Names:MG334
    OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
    Taxonomic identifieri243273 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000807: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 837837Valine--tRNA ligasePRO_0000106230Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi243273.MG_334.

    Structurei

    3D structure databases

    ProteinModelPortaliP47576.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili767 – 83771UniRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi46 – 5611"HIGH" regionAdd
    BLAST
    Motifi514 – 5185"KMSKS" region

    Domaini

    ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.UniRule annotation
    The C-terminal coiled-coil domain is crucial for aminoacylation activity.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.UniRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0525.
    KOiK01873.
    OMAiKHELLEQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02004. Val_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR010978. tRNA-bd_arm.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR019499. Val-tRNA_synth_tRNA-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR002303. Valyl-tRNA_ligase.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF10458. Val_tRNA-synt_C. 1 hit.
    [Graphical view]
    PRINTSiPR00986. TRNASYNTHVAL.
    SUPFAMiSSF46589. SSF46589. 1 hit.
    SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00422. valS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47576-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKDKFSFQKN YDFNLVSDGL YEIWNNAGFF KPKDKNNSFT AILPPPNLTG    50
    TLHIGHAFEV SITDQIMRFK KMQGFSINWI PGFDHAGIAT QTKYEKIALK 100
    ENQKYFDADD DKKSEMIMNW ALNQSEIIKN QLKSLGVCLN WSETKFTLSE 150
    QANKIVNNCF KNLYENGFIY QAYTLVNWDT KLNTAISNIE VINKPVNQHL 200
    HYVVYKLAND SKQELIVATT RPETIFADVC LLVNPKDKRY TNFWNKLVVN 250
    PLTGKQIPVV TDSYVDIKFG TGILKCTPAH DFNDYEINTK YKFDFLSCID 300
    SNGILNQNAS KFQGLSVLQA RNKIVKWLEK NKLLVKSIPL TSNVGFSERS 350
    GTVVEPMLSK QWFVDLPKLK DHLYLKKYPD FIPKRFNKQV SNWLNKLKPW 400
    CISRQLIWGH KIPVWFENNT GEIVVGEKPS KNLQNYTRSK DVLDTWFSSS 450
    LWPLICLNWE QDDSFHETEL LVTGYDILFF WVLRMLFNSF FETKKLPFKT 500
    VLIHGLVRDE QNRKMSKSLN NGIDPVDLIR NYGADAVRLF LCSNHTPGDD 550
    LIFSEQKIKS AWNFLNKLWN VTKFVIQLEN DQEISYDLDK LSLSETWILA 600
    KLDKVIQKIT KLLDKFQLAL ANQILVKFVW DDFCNTFIEA IKKEPNQLKP 650
    QLFYTAKSVL SNIAILLSIT VPFLSERIYQ QFNNKSVMQA TWPLATKIKI 700
    PKLFDLVLAA INDLRNYRKQ YMLNSQQKLV VILSGKNAVD VKQYFNFSWI 750
    ELKIETNKKV SFKYQIVDDT TQRLKSLQKQ QAFFESEVKR SQAIVKNKSF 800
    LEKAPKEKVK SEFLKLEEYQ KKLTETNQLI AKLTKAH 837
    Length:837
    Mass (Da):97,464
    Last modified:February 1, 1996 - v1
    Checksum:i7F2FD1FE95D3D8C5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71557.1.
    U02249 Genomic DNA. Translation: AAD12537.1.
    PIRiI64236.
    RefSeqiNP_073002.1. NC_000908.2.
    WP_010869437.1. NC_000908.2.

    Genome annotation databases

    EnsemblBacteriaiAAC71557; AAC71557; MG_334.
    GeneIDi875510.
    KEGGimge:MG_334.
    PATRICi20010224. VBIMycGen98045_0389.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71557.1 .
    U02249 Genomic DNA. Translation: AAD12537.1 .
    PIRi I64236.
    RefSeqi NP_073002.1. NC_000908.2.
    WP_010869437.1. NC_000908.2.

    3D structure databases

    ProteinModelPortali P47576.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243273.MG_334.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC71557 ; AAC71557 ; MG_334 .
    GeneIDi 875510.
    KEGGi mge:MG_334.
    PATRICi 20010224. VBIMycGen98045_0389.

    Phylogenomic databases

    eggNOGi COG0525.
    KOi K01873.
    OMAi KHELLEQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci MGEN243273:GH2R-382-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02004. Val_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR010978. tRNA-bd_arm.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR019499. Val-tRNA_synth_tRNA-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR002303. Valyl-tRNA_ligase.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF10458. Val_tRNA-synt_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00986. TRNASYNTHVAL.
    SUPFAMi SSF46589. SSF46589. 1 hit.
    SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00422. valS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33530 / G-37 / NCTC 10195.
    2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
      Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
      J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 801-837.
      Strain: ATCC 33530 / G-37 / NCTC 10195.

    Entry informationi

    Entry nameiSYV_MYCGE
    AccessioniPrimary (citable) accession number: P47576
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Mycoplasma genitalium
      Mycoplasma genitalium (strain G-37): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3