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P47576

- SYV_MYCGE

UniProt

P47576 - SYV_MYCGE

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Protein

Valine--tRNA ligase

Gene
valS, MG334
Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity.UniRule annotation

Catalytic activityi

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei517 – 5171ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. valine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. valyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-382-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Valine--tRNA ligase (EC:6.1.1.9)
Alternative name(s):
Valyl-tRNA synthetase
Short name:
ValRS
Gene namesi
Name:valS
Ordered Locus Names:MG334
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 837837Valine--tRNA ligaseUniRule annotationPRO_0000106230Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi243273.MG_334.

Structurei

3D structure databases

ProteinModelPortaliP47576.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili767 – 83771 Reviewed predictionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 5611"HIGH" regionUniRule annotationAdd
BLAST
Motifi514 – 5185"KMSKS" regionUniRule annotation

Domaini

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity.UniRule annotation
The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity.UniRule annotation

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0525.
KOiK01873.
OMAiKHELLEQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF46589. SSF46589. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47576-1 [UniParc]FASTAAdd to Basket

« Hide

MKDKFSFQKN YDFNLVSDGL YEIWNNAGFF KPKDKNNSFT AILPPPNLTG    50
TLHIGHAFEV SITDQIMRFK KMQGFSINWI PGFDHAGIAT QTKYEKIALK 100
ENQKYFDADD DKKSEMIMNW ALNQSEIIKN QLKSLGVCLN WSETKFTLSE 150
QANKIVNNCF KNLYENGFIY QAYTLVNWDT KLNTAISNIE VINKPVNQHL 200
HYVVYKLAND SKQELIVATT RPETIFADVC LLVNPKDKRY TNFWNKLVVN 250
PLTGKQIPVV TDSYVDIKFG TGILKCTPAH DFNDYEINTK YKFDFLSCID 300
SNGILNQNAS KFQGLSVLQA RNKIVKWLEK NKLLVKSIPL TSNVGFSERS 350
GTVVEPMLSK QWFVDLPKLK DHLYLKKYPD FIPKRFNKQV SNWLNKLKPW 400
CISRQLIWGH KIPVWFENNT GEIVVGEKPS KNLQNYTRSK DVLDTWFSSS 450
LWPLICLNWE QDDSFHETEL LVTGYDILFF WVLRMLFNSF FETKKLPFKT 500
VLIHGLVRDE QNRKMSKSLN NGIDPVDLIR NYGADAVRLF LCSNHTPGDD 550
LIFSEQKIKS AWNFLNKLWN VTKFVIQLEN DQEISYDLDK LSLSETWILA 600
KLDKVIQKIT KLLDKFQLAL ANQILVKFVW DDFCNTFIEA IKKEPNQLKP 650
QLFYTAKSVL SNIAILLSIT VPFLSERIYQ QFNNKSVMQA TWPLATKIKI 700
PKLFDLVLAA INDLRNYRKQ YMLNSQQKLV VILSGKNAVD VKQYFNFSWI 750
ELKIETNKKV SFKYQIVDDT TQRLKSLQKQ QAFFESEVKR SQAIVKNKSF 800
LEKAPKEKVK SEFLKLEEYQ KKLTETNQLI AKLTKAH 837
Length:837
Mass (Da):97,464
Last modified:February 1, 1996 - v1
Checksum:i7F2FD1FE95D3D8C5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71557.1.
U02249 Genomic DNA. Translation: AAD12537.1.
PIRiI64236.
RefSeqiNP_073002.1. NC_000908.2.
WP_010869437.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71557; AAC71557; MG_334.
GeneIDi875510.
KEGGimge:MG_334.
PATRICi20010224. VBIMycGen98045_0389.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71557.1 .
U02249 Genomic DNA. Translation: AAD12537.1 .
PIRi I64236.
RefSeqi NP_073002.1. NC_000908.2.
WP_010869437.1. NC_000908.2.

3D structure databases

ProteinModelPortali P47576.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243273.MG_334.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC71557 ; AAC71557 ; MG_334 .
GeneIDi 875510.
KEGGi mge:MG_334.
PATRICi 20010224. VBIMycGen98045_0389.

Phylogenomic databases

eggNOGi COG0525.
KOi K01873.
OMAi KHELLEQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MGEN243273:GH2R-382-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02004. Val_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view ]
PRINTSi PR00986. TRNASYNTHVAL.
SUPFAMi SSF46589. SSF46589. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00422. valS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 801-837.
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiSYV_MYCGE
AccessioniPrimary (citable) accession number: P47576
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi