valS

Functionⁱ

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.UniRule annotation

Catalytic activityⁱ

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).UniRule annotation

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	517	ATPUniRule annotation		1

GO - Molecular functionⁱ

aminoacyl-tRNA editing activity Source: InterPro
ATP binding Source: UniProtKB-KW
valine-tRNA ligase activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

valyl-tRNA aminoacylation Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Aminoacyl-tRNA synthetase, Ligase
Biological process	Protein biosynthesis
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	MGEN243273:G1GJ2-416-MONOMER

BioCycⁱ

MGEN243273:G1GJ2-416-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Valine--tRNA ligaseUniRule annotation (EC:6.1.1.9UniRule annotation) Alternative name(s): Valyl-tRNA synthetaseUniRule annotation Short name: ValRSUniRule annotation
Gene namesⁱ	Name:valSUniRule annotation Ordered Locus Names:MG334
Organismⁱ	Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifierⁱ	243273 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium
Proteomesⁱ	UP000000807 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_02004

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000106230	1 – 837	Valine--tRNA ligaseAdd BLAST		837

Interactionⁱ

Subunit structureⁱ

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGⁱ	243273.MgenG_010200003096

STRINGⁱ

243273.MgenG_010200003096

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P47576
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	767 – 837	UniRule annotationAdd BLAST		71

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	46 – 56	"HIGH" regionAdd BLAST		11
Motifⁱ	514 – 518	"KMSKS" region		5

Domainⁱ

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.UniRule annotation

The C-terminal coiled-coil domain is crucial for aminoacylation activity.UniRule annotation

Sequence similaritiesⁱ

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.UniRule annotation

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

eggNOGⁱ	ENOG4105CA4 Bacteria COG0525 LUCA
KEGG Orthology (KO) More...KOⁱ	K01873
OMAⁱ	RQWYIRN
Database of Orthologous Groups More...OrthoDBⁱ	POG091H01HY

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd07962 Anticodon_Ia_Val, 1 hit
Gene3Dⁱ	1.10.287.3801 hit 3.40.50.6202 hits 3.90.740.101 hit
HAMAPⁱ	MF_02004 Val_tRNA_synth_type1, 1 hit
InterProⁱ	View protein in InterPro IPR001412 aa-tRNA-synth_I_CS IPR002300 aa-tRNA-synth_Ia IPR033705 Anticodon_Ia_Val IPR013155 M/V/L/I-tRNA-synth_anticd-bd IPR014729 Rossmann-like_a/b/a_fold IPR010978 tRNA-bd_arm IPR009080 tRNAsynth_Ia_anticodon-bd IPR037118 Val-tRNA_synth_C_sf IPR019499 Val-tRNA_synth_tRNA-bd IPR009008 Val/Leu/Ile-tRNA-synth_edit IPR002303 Valyl-tRNA_ligase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08264 Anticodon_1, 1 hit PF00133 tRNA-synt_1, 1 hit PF10458 Val_tRNA-synt_C, 1 hit
PRINTSⁱ	PR00986 TRNASYNTHVAL
SUPFAMⁱ	SSF46589 SSF46589, 1 hit SSF47323 SSF47323, 1 hit SSF50677 SSF50677, 1 hit
TIGRFAMsⁱ	TIGR00422 valS, 1 hit
PROSITEⁱ	View protein in PROSITE PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P47576-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKDKFSFQKN YDFNLVSDGL YEIWNNAGFF KPKDKNNSFT AILPPPNLTG 
        60         70         80         90        100
TLHIGHAFEV SITDQIMRFK KMQGFSINWI PGFDHAGIAT QTKYEKIALK 
       110        120        130        140        150
ENQKYFDADD DKKSEMIMNW ALNQSEIIKN QLKSLGVCLN WSETKFTLSE 
       160        170        180        190        200
QANKIVNNCF KNLYENGFIY QAYTLVNWDT KLNTAISNIE VINKPVNQHL 
       210        220        230        240        250
HYVVYKLAND SKQELIVATT RPETIFADVC LLVNPKDKRY TNFWNKLVVN 
       260        270        280        290        300
PLTGKQIPVV TDSYVDIKFG TGILKCTPAH DFNDYEINTK YKFDFLSCID 
       310        320        330        340        350
SNGILNQNAS KFQGLSVLQA RNKIVKWLEK NKLLVKSIPL TSNVGFSERS 
       360        370        380        390        400
GTVVEPMLSK QWFVDLPKLK DHLYLKKYPD FIPKRFNKQV SNWLNKLKPW 
       410        420        430        440        450
CISRQLIWGH KIPVWFENNT GEIVVGEKPS KNLQNYTRSK DVLDTWFSSS 
       460        470        480        490        500
LWPLICLNWE QDDSFHETEL LVTGYDILFF WVLRMLFNSF FETKKLPFKT 
       510        520        530        540        550
VLIHGLVRDE QNRKMSKSLN NGIDPVDLIR NYGADAVRLF LCSNHTPGDD 
       560        570        580        590        600
LIFSEQKIKS AWNFLNKLWN VTKFVIQLEN DQEISYDLDK LSLSETWILA 
       610        620        630        640        650
KLDKVIQKIT KLLDKFQLAL ANQILVKFVW DDFCNTFIEA IKKEPNQLKP 
       660        670        680        690        700
QLFYTAKSVL SNIAILLSIT VPFLSERIYQ QFNNKSVMQA TWPLATKIKI 
       710        720        730        740        750
PKLFDLVLAA INDLRNYRKQ YMLNSQQKLV VILSGKNAVD VKQYFNFSWI 
       760        770        780        790        800
ELKIETNKKV SFKYQIVDDT TQRLKSLQKQ QAFFESEVKR SQAIVKNKSF 
       810        820        830 
LEKAPKEKVK SEFLKLEEYQ KKLTETNQLI AKLTKAH

Length:837

Mass (Da):97,464

Last modified:February 1, 1996 - v1

Checksum:ⁱ7F2FD1FE95D3D8C5

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L43967 Genomic DNA Translation: AAC71557.1 U02249 Genomic DNA Translation: AAD12537.1
PIRⁱ	I64236
NCBI Reference Sequences More...RefSeqⁱ	WP_010869437.1, NC_000908.2

Genome annotation databases

EnsemblBacteriaⁱ	AAC71557; AAC71557; MG_334
KEGGⁱ	mge:MG_334

Similar proteinsⁱ

50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P47576	P75304 UPI0006BA21CE A0A0H3DMS3 UPI000A29F63D UPI00027B4072 UPI00027B3E43 UPI00027B3D33	Mycoplasma pneumoniae (strain ATCC 29342 / M129) Mycoplasma pneumoniae Mycoplasma pneumoniae (strain ATCC 15531 / DSM 22911 / NBRC 14401 / NCTC 10119 / FH) Mycoplasma genitalium	838	UniRef50_P75304	Cluster: Valine--tRNA ligase	8

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L43967 Genomic DNA Translation: AAC71557.1 U02249 Genomic DNA Translation: AAD12537.1
PIRⁱ	I64236
NCBI Reference Sequences More...RefSeqⁱ	WP_010869437.1, NC_000908.2

3D structure databases

ProteinModelPortalⁱ	P47576
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	243273.MgenG_010200003096

STRINGⁱ

243273.MgenG_010200003096

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC71557; AAC71557; MG_334
KEGGⁱ	mge:MG_334

Phylogenomic databases

eggNOGⁱ	ENOG4105CA4 Bacteria COG0525 LUCA
KEGG Orthology (KO) More...KOⁱ	K01873
OMAⁱ	RQWYIRN
Database of Orthologous Groups More...OrthoDBⁱ	POG091H01HY

Enzyme and pathway databases

BioCycⁱ	MGEN243273:G1GJ2-416-MONOMER

BioCycⁱ

MGEN243273:G1GJ2-416-MONOMER

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd07962 Anticodon_Ia_Val, 1 hit
Gene3Dⁱ	1.10.287.3801 hit 3.40.50.6202 hits 3.90.740.101 hit
HAMAPⁱ	MF_02004 Val_tRNA_synth_type1, 1 hit
InterProⁱ	View protein in InterPro IPR001412 aa-tRNA-synth_I_CS IPR002300 aa-tRNA-synth_Ia IPR033705 Anticodon_Ia_Val IPR013155 M/V/L/I-tRNA-synth_anticd-bd IPR014729 Rossmann-like_a/b/a_fold IPR010978 tRNA-bd_arm IPR009080 tRNAsynth_Ia_anticodon-bd IPR037118 Val-tRNA_synth_C_sf IPR019499 Val-tRNA_synth_tRNA-bd IPR009008 Val/Leu/Ile-tRNA-synth_edit IPR002303 Valyl-tRNA_ligase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08264 Anticodon_1, 1 hit PF00133 tRNA-synt_1, 1 hit PF10458 Val_tRNA-synt_C, 1 hit
PRINTSⁱ	PR00986 TRNASYNTHVAL
SUPFAMⁱ	SSF46589 SSF46589, 1 hit SSF47323 SSF47323, 1 hit SSF50677 SSF50677, 1 hit
TIGRFAMsⁱ	TIGR00422 valS, 1 hit
PROSITEⁱ	View protein in PROSITE PS00178 AA_TRNA_LIGASE_I, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SYV_MYCGE
Accessionⁱ	P47576Primary (citable) accession number: P47576
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
	Last sequence update:	February 1, 1996
	Last modified:	February 28, 2018
	This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P47576 (SYV_MYCGE)

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Valine--tRNA ligase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Coiled coil

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ