P47547 (DNAK_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chaperone protein DnaK

Alternative name(s):
HSP70
Heat shock 70 kDa protein
Heat shock protein 70

Gene names

Name:	dnaK
Synonyms:	hsp70
Ordered Locus Names:	MG305

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts as a chaperone By similarity.
Induction	By stress conditions e.g. heat shock By similarity.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Biological process	Stress response
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 595	595	Chaperone protein DnaK HAMAP-Rule MF_00332		PRO_0000078490
Amino acid modifications
	Modified residue	182	1	Phosphothreonine; by autocatalysis By similarity
Experimental info
	Sequence conflict	447 – 460	14	PKGKP…TFSLD → LKVNPKLRLPLVWM Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47547 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 8EB48D058A009FB7	FASTA	595	65,051
	10 20 30 40 50 60 MSADNGLIIG IDLGTTNSCV SVMEGGRPVV LENPEGKRTT PSIVSYKNNE IIVGDAAKRQ 70 80 90 100 110 120 MVTNPNTIVS IKRLMGTSNK VKVQNADGTT KELSPEQVSA QILSYLKDFA EKKIGKKISR 130 140 150 160 170 180 AVITVPAYFN DAERNATKTA GKIAGLNVER IINEPTAAAL AYGIDKASRE MKVLVYDLGG 190 200 210 220 230 240 GTFDVSLLDI AEGTFEVLAT AGDNRLGGDD WDNKIIEYIS AYIAKEHQGL NLSKDKMAMQ 250 260 270 280 290 300 RLKEAAERAK IELSAQLETI ISLPFLTVTQ KGPVNVELKL TRAKFEELTK PLLERTRNPI 310 320 330 340 350 360 SDVIKEAKIK PEEINEILLV GGSTRMPAVQ KLVESMVPGK KPNRSINPDE VVAIGAAIQG 370 380 390 400 410 420 GVLRGDVKDV LLLDVTPLTL SIETLGGVAT PLIKRNTTIP VSKSQIFSTA QDNQESVDVV 430 440 450 460 470 480 VCQGERPMSR DNKSLGRFNL GGIQPAPKGK PQIEITFSLD ANGILNVKAK DLTTQKENSI 490 500 510 520 530 540 TISDNGNLSE EEIQKMIRDA EANKERDNII RERIELRNEG EGIVNTIKEI LASPDAKNFP 550 560 570 580 590 KEEKEKLEKL TGNIDAAIKA NDYAKLKVEI ENFKKWREEM AKKYNPTGEQ GPQAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 353-460. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71527.1. U02204 Genomic DNA. Translation: AAD12493.1.
PIR	G64233.
RefSeq	NP_072972.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47547.
SMR	P47547. Positions 8-516.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_305.
Proteomic databases
PRIDE	P47547.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71527; AAC71527; MG_305.
GeneID	875435.
KEGG	mge:MG_305.
PATRIC	20010140. VBIMycGen98045_0356.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0443.
KO	K04043.
OMA	PENTIYA.
ProtClustDB	PRK00290.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-354-MONOMER.
Family and domain databases
HAMAP	MF_00332. DnaK.
InterPro	IPR012725. Chaperone_DnaK. IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
TIGRFAMs	TIGR02350. prok_dnaK. 1 hit.
PROSITE	PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAK_MYCGE

Accession

Primary (citable) accession number: P47547
Secondary accession number(s): Q49321

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program