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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gapA)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42SubstrateBy similarity1
Binding sitei128SubstrateBy similarity1
Binding sitei170SubstrateBy similarity1
Binding sitei221ATPBy similarity1
Binding sitei344ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi371 – 374ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:pgk
Ordered Locus Names:MG300
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001459651 – 416Phosphoglycerate kinaseAdd BLAST416

Proteomic databases

PRIDEiP47542.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP47542.
SMRiP47542.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 29Substrate bindingBy similarity3
Regioni65 – 68Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

KOiK00927.
OMAiDMIFDIG.
OrthoDBiPOG091H00YN.

Family and domain databases

Gene3Di3.40.50.1260. 2 hits.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiView protein in InterPro
IPR001576. Phosphoglycerate_kinase.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiView protein in Pfam
PF00162. PGK. 1 hit.
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiView protein in PROSITE
PS00111. PGLYCERATE_KINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P47542-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSSNMLNFK TLQAIDFQNK TVVLRSDFNV PMINGVISDS ERILAGLDTI
60 70 80 90 100
KFLVKKNCKI VLLSHLSRIK SLEDKLNNKK SLKPVAELLQ QLLPTVKVQF
110 120 130 140 150
SCKNTGAEVK QKVQALAFGE ILLLENTRYC DVNDKGEIVK LESKNDPELA
160 170 180 190 200
KFWASLGEIF VNDAFGTAHR KHASNAGIAK YVAKSCIGFL MEKELKNLSY
210 220 230 240 250
LIQSPQKPFV VVLGGAKVSD KLKVVENLLK LADNILIGGG MVNTFLKAKG
260 270 280 290 300
KATANSLVEK ELIDVAKQIL DKDTHNKIVL AIDQVMGSEF KDQTGITLDV
310 320 330 340 350
SDKIQEQYQS YMSLDVGSKT IALFESYLKT AKTIFWNGPL GVFEFTNFAK
360 370 380 390 400
GTSKIGEIIA KNKTAFSVIG GGDSAAAVKQ MQLSDQFSFI STGGGASLAL
410
IGGEELVGIS DIQKNS
Length:416
Mass (Da):45,380
Last modified:February 1, 1996 - v1
Checksum:i2A76E2C294BD92E6
GO

Sequence cautioni

The sequence AAD12464 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti204 – 206SPQ → KPT in AAA03379 (PubMed:8253680).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71522.1.
U02226 Genomic DNA. Translation: AAA03379.1.
U02234 Genomic DNA. Translation: AAA03386.1.
U02178 Genomic DNA. Translation: AAD12464.1. Different initiation.
PIRiB64233.
RefSeqiWP_010869417.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71522; AAC71522; MG_300.
KEGGimge:MG_300.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPGK_MYCGE
AccessioniPrimary (citable) accession number: P47542
Secondary accession number(s): Q49338, Q59521
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 7, 2017
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families