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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gapA)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37SubstrateBy similarity1
Binding sitei123SubstrateBy similarity1
Binding sitei165SubstrateBy similarity1
Binding sitei216ATPBy similarity1
Binding sitei339ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi366 – 369ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:pgk
Ordered Locus Names:MG300
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Probably essential, it was not disrupted in a global transposon mutagenesis study.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001459651 – 411Phosphoglycerate kinaseAdd BLAST411

Proteomic databases

PRIDEiP47542

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP47542
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 24Substrate bindingBy similarity3
Regioni60 – 63Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

KOiK00927
OMAiDMIFDIG
OrthoDBiPOG091H00YN

Family and domain databases

Gene3Di3.40.50.1260, 3 hits
HAMAPiMF_00145 Phosphoglyc_kinase, 1 hit
InterProiView protein in InterPro
IPR001576 Phosphoglycerate_kinase
IPR015911 Phosphoglycerate_kinase_CS
IPR015824 Phosphoglycerate_kinase_N
IPR036043 Phosphoglycerate_kinase_sf
PANTHERiPTHR11406 PTHR11406, 1 hit
PfamiView protein in Pfam
PF00162 PGK, 1 hit
PIRSFiPIRSF000724 Pgk, 1 hit
PRINTSiPR00477 PHGLYCKINASE
SUPFAMiSSF53748 SSF53748, 1 hit
PROSITEiView protein in PROSITE
PS00111 PGLYCERATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P47542-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNFKTLQAI DFQNKTVVLR SDFNVPMING VISDSERILA GLDTIKFLVK
60 70 80 90 100
KNCKIVLLSH LSRIKSLEDK LNNKKSLKPV AELLQQLLPT VKVQFSCKNT
110 120 130 140 150
GAEVKQKVQA LAFGEILLLE NTRYCDVNDK GEIVKLESKN DPELAKFWAS
160 170 180 190 200
LGEIFVNDAF GTAHRKHASN AGIAKYVAKS CIGFLMEKEL KNLSYLIQSP
210 220 230 240 250
QKPFVVVLGG AKVSDKLKVV ENLLKLADNI LIGGGMVNTF LKAKGKATAN
260 270 280 290 300
SLVEKELIDV AKQILDKDTH NKIVLAIDQV MGSEFKDQTG ITLDVSDKIQ
310 320 330 340 350
EQYQSYMSLD VGSKTIALFE SYLKTAKTIF WNGPLGVFEF TNFAKGTSKI
360 370 380 390 400
GEIIAKNKTA FSVIGGGDSA AAVKQMQLSD QFSFISTGGG ASLALIGGEE
410
LVGISDIQKN S
Length:411
Mass (Da):44,847
Last modified:January 31, 2018 - v2
Checksum:i147781D8A519D1E7
GO

Sequence cautioni

The sequence AAD12464 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti199 – 201SPQ → KPT in AAA03379 (PubMed:8253680).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA Translation: AAC71522.2
U02226 Genomic DNA Translation: AAA03379.1
U02234 Genomic DNA Translation: AAA03386.1
U02178 Genomic DNA Translation: AAD12464.1 Different initiation.
PIRiB64233
RefSeqiWP_010869417.1, NC_000908.2

Genome annotation databases

EnsemblBacteriaiAAC71522; AAC71522; MG_300
KEGGimge:MG_300

Similar proteinsi

Entry informationi

Entry nameiPGK_MYCGE
AccessioniPrimary (citable) accession number: P47542
Secondary accession number(s): Q49338, Q59521
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 31, 2018
Last modified: March 28, 2018
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health