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P47534

- SYA_MYCGE

UniProt

P47534 - SYA_MYCGE

Protein

Alanine--tRNA ligase

Gene

alaS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.UniRule annotation

    Catalytic activityi

    ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi568 – 5681ZincUniRule annotation
    Metal bindingi572 – 5721ZincUniRule annotation
    Metal bindingi672 – 6721ZincUniRule annotation
    Metal bindingi676 – 6761ZincUniRule annotation

    GO - Molecular functioni

    1. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-KW
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

    Enzyme and pathway databases

    BioCyciMGEN243273:GH2R-328-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine--tRNA ligaseUniRule annotation (EC:6.1.1.7UniRule annotation)
    Alternative name(s):
    Alanyl-tRNA synthetaseUniRule annotation
    Short name:
    AlaRSUniRule annotation
    Gene namesi
    Name:alaSUniRule annotation
    Ordered Locus Names:MG292
    OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
    Taxonomic identifieri243273 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000807: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 900900Alanine--tRNA ligasePRO_0000075149Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi243273.MG_292.

    Structurei

    3D structure databases

    ProteinModelPortaliP47534.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0013.
    KOiK01872.
    OMAiWASKGSP.
    OrthoDBiEOG6Q2SQ2.

    Family and domain databases

    HAMAPiMF_00036_B. Ala_tRNA_synth_B.
    InterProiIPR002318. Ala-tRNA-lgiase_IIc.
    IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
    IPR018165. Ala-tRNA-synth_IIc_core.
    IPR018164. Ala-tRNA-synth_IIc_N.
    IPR023033. Ala_tRNA_ligase_euk/bac.
    IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
    IPR009000. Transl_B-barrel.
    IPR012947. tRNA_SAD.
    [Graphical view]
    PfamiPF01411. tRNA-synt_2c. 1 hit.
    PF07973. tRNA_SAD. 1 hit.
    [Graphical view]
    PRINTSiPR00980. TRNASYNTHALA.
    SMARTiSM00863. tRNA_SAD. 1 hit.
    [Graphical view]
    SUPFAMiSSF101353. SSF101353. 1 hit.
    SSF50447. SSF50447. 1 hit.
    SSF55186. SSF55186. 1 hit.
    TIGRFAMsiTIGR00344. alaS. 1 hit.
    PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47534-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNWTTDKVRQ TWLDYFAKKD HLVLASKSLI PINDPSLLWI NSGVATLKDY    50
    FSARKTPPSK RLVNAQICLR VNDIENVGFT SRHQTLFEML GNFSIGDYFK 100
    TEAIDFAFDL LVNYYQLDPK RFYITVYEDD ETTYKRWIKH KIDKNHIIKC 150
    DKSRNFWDLG LGPCGPCTEI YYDRGEKFDP KKIGEKLFFE DIENDRYVEI 200
    WNIVFSQFNN DGNGNYTELA QKNIDTGAGI ERLVSVLQNS PTNFDTDIFL 250
    KLIKIIEAFC PFKYDPNSYF TFDPQKVKEQ SYFRIIADHF KAITFTISEG 300
    VLPGPNERNY VVRRLLRRAL IACKKLQLNL AFIEKIIDEI IASYENYYQH 350
    LKAKNETVKQ VVLKEINAFN KTIDLGLVLF EKSVKNNTLT PQLTFQLNET 400
    YGFPVEIIRE LVNQKGLTID WTVFDQLMAK HRSISKQNNQ TINFEKQNIN 450
    LVNFKTKSTF FYHKNKINAK VIGLFDENYL PVKELNNQSG YVVFDQTVLY 500
    ATSGGQRYDE GSCINHSNNN DQKISFQGVF KGPNKQHFHY FLVGSFKLND 550
    QVTLSHDETW RKLAANNHSL EHLLHAALQK EIDPLIKQSG AFKSAQKATI 600
    DFNLNRHLTR NELEKVENKI RSLIKQKISS KEIFTDFEGS QKLNAIAYFE 650
    EEYSQHEILR VIRFGDYSVE LCGGTHVANT ASIEDCFITD FYSLGAGRWR 700
    IEIISSNETI NNYLKAENQK LIQLKSELEK VLSLIDSSIF KVELKELQQR 750
    LDKFILPEKI TQLRDASDTL LALKNDINQL KTKNYKVSQQ ALALSIKKQL 800
    LSLVDENKSY VIATFNDVEP KLLLQTLHDV FNQNQTKNFL IINQFNESNS 850
    FIVIGNKTTT IIEKLRNSFN LKGGGNDKLF RGSFQDNVTP QKLNELFQNK 900
    Length:900
    Mass (Da):104,302
    Last modified:February 1, 1996 - v1
    Checksum:iAA54520BFB3949A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71513.1.
    PIRiC64232.
    RefSeqiNP_072959.1. NC_000908.2.
    WP_010869412.1. NC_000908.2.

    Genome annotation databases

    EnsemblBacteriaiAAC71513; AAC71513; MG_292.
    GeneIDi875339.
    KEGGimge:MG_292.
    PATRICi20010114. VBIMycGen98045_0343.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71513.1 .
    PIRi C64232.
    RefSeqi NP_072959.1. NC_000908.2.
    WP_010869412.1. NC_000908.2.

    3D structure databases

    ProteinModelPortali P47534.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243273.MG_292.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC71513 ; AAC71513 ; MG_292 .
    GeneIDi 875339.
    KEGGi mge:MG_292.
    PATRICi 20010114. VBIMycGen98045_0343.

    Phylogenomic databases

    eggNOGi COG0013.
    KOi K01872.
    OMAi WASKGSP.
    OrthoDBi EOG6Q2SQ2.

    Enzyme and pathway databases

    BioCyci MGEN243273:GH2R-328-MONOMER.

    Family and domain databases

    HAMAPi MF_00036_B. Ala_tRNA_synth_B.
    InterProi IPR002318. Ala-tRNA-lgiase_IIc.
    IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
    IPR018165. Ala-tRNA-synth_IIc_core.
    IPR018164. Ala-tRNA-synth_IIc_N.
    IPR023033. Ala_tRNA_ligase_euk/bac.
    IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
    IPR009000. Transl_B-barrel.
    IPR012947. tRNA_SAD.
    [Graphical view ]
    Pfami PF01411. tRNA-synt_2c. 1 hit.
    PF07973. tRNA_SAD. 1 hit.
    [Graphical view ]
    PRINTSi PR00980. TRNASYNTHALA.
    SMARTi SM00863. tRNA_SAD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101353. SSF101353. 1 hit.
    SSF50447. SSF50447. 1 hit.
    SSF55186. SSF55186. 1 hit.
    TIGRFAMsi TIGR00344. alaS. 1 hit.
    PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33530 / G-37 / NCTC 10195.

    Entry informationi

    Entry nameiSYA_MYCGE
    AccessioniPrimary (citable) accession number: P47534
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Mycoplasma genitalium
      Mycoplasma genitalium (strain G-37): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3