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Protein

Alanine--tRNA ligase

Gene

alaS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.UniRule annotation

Catalytic activityi

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi568 – 5681ZincUniRule annotation
Metal bindingi572 – 5721ZincUniRule annotation
Metal bindingi672 – 6721ZincUniRule annotation
Metal bindingi676 – 6761ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-328-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--tRNA ligaseUniRule annotation (EC:6.1.1.7UniRule annotation)
Alternative name(s):
Alanyl-tRNA synthetaseUniRule annotation
Short name:
AlaRSUniRule annotation
Gene namesi
Name:alaSUniRule annotation
Ordered Locus Names:MG292
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Alanine--tRNA ligasePRO_0000075149Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP47534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0013.
KOiK01872.
OMAiGLERIYW.
OrthoDBiEOG6Q2SQ2.

Family and domain databases

HAMAPiMF_00036_B. Ala_tRNA_synth_B.
InterProiIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR00980. TRNASYNTHALA.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00344. alaS. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNWTTDKVRQ TWLDYFAKKD HLVLASKSLI PINDPSLLWI NSGVATLKDY
60 70 80 90 100
FSARKTPPSK RLVNAQICLR VNDIENVGFT SRHQTLFEML GNFSIGDYFK
110 120 130 140 150
TEAIDFAFDL LVNYYQLDPK RFYITVYEDD ETTYKRWIKH KIDKNHIIKC
160 170 180 190 200
DKSRNFWDLG LGPCGPCTEI YYDRGEKFDP KKIGEKLFFE DIENDRYVEI
210 220 230 240 250
WNIVFSQFNN DGNGNYTELA QKNIDTGAGI ERLVSVLQNS PTNFDTDIFL
260 270 280 290 300
KLIKIIEAFC PFKYDPNSYF TFDPQKVKEQ SYFRIIADHF KAITFTISEG
310 320 330 340 350
VLPGPNERNY VVRRLLRRAL IACKKLQLNL AFIEKIIDEI IASYENYYQH
360 370 380 390 400
LKAKNETVKQ VVLKEINAFN KTIDLGLVLF EKSVKNNTLT PQLTFQLNET
410 420 430 440 450
YGFPVEIIRE LVNQKGLTID WTVFDQLMAK HRSISKQNNQ TINFEKQNIN
460 470 480 490 500
LVNFKTKSTF FYHKNKINAK VIGLFDENYL PVKELNNQSG YVVFDQTVLY
510 520 530 540 550
ATSGGQRYDE GSCINHSNNN DQKISFQGVF KGPNKQHFHY FLVGSFKLND
560 570 580 590 600
QVTLSHDETW RKLAANNHSL EHLLHAALQK EIDPLIKQSG AFKSAQKATI
610 620 630 640 650
DFNLNRHLTR NELEKVENKI RSLIKQKISS KEIFTDFEGS QKLNAIAYFE
660 670 680 690 700
EEYSQHEILR VIRFGDYSVE LCGGTHVANT ASIEDCFITD FYSLGAGRWR
710 720 730 740 750
IEIISSNETI NNYLKAENQK LIQLKSELEK VLSLIDSSIF KVELKELQQR
760 770 780 790 800
LDKFILPEKI TQLRDASDTL LALKNDINQL KTKNYKVSQQ ALALSIKKQL
810 820 830 840 850
LSLVDENKSY VIATFNDVEP KLLLQTLHDV FNQNQTKNFL IINQFNESNS
860 870 880 890 900
FIVIGNKTTT IIEKLRNSFN LKGGGNDKLF RGSFQDNVTP QKLNELFQNK
Length:900
Mass (Da):104,302
Last modified:February 1, 1996 - v1
Checksum:iAA54520BFB3949A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71513.1.
PIRiC64232.
RefSeqiNP_072959.1. NC_000908.2.
WP_010869412.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71513; AAC71513; MG_292.
KEGGimge:MG_292.
PATRICi20010114. VBIMycGen98045_0343.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71513.1.
PIRiC64232.
RefSeqiNP_072959.1. NC_000908.2.
WP_010869412.1. NC_000908.2.

3D structure databases

ProteinModelPortaliP47534.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71513; AAC71513; MG_292.
KEGGimge:MG_292.
PATRICi20010114. VBIMycGen98045_0343.

Phylogenomic databases

eggNOGiCOG0013.
KOiK01872.
OMAiGLERIYW.
OrthoDBiEOG6Q2SQ2.

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-328-MONOMER.

Family and domain databases

HAMAPiMF_00036_B. Ala_tRNA_synth_B.
InterProiIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR00980. TRNASYNTHALA.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00344. alaS. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiSYA_MYCGE
AccessioniPrimary (citable) accession number: P47534
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.