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P47534

- SYA_MYCGE

UniProt

P47534 - SYA_MYCGE

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Protein

Alanine--tRNA ligase

Gene

alaS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.UniRule annotation

Catalytic activityi

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi568 – 5681ZincUniRule annotation
Metal bindingi572 – 5721ZincUniRule annotation
Metal bindingi672 – 6721ZincUniRule annotation
Metal bindingi676 – 6761ZincUniRule annotation

GO - Molecular functioni

  1. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-KW
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-328-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--tRNA ligaseUniRule annotation (EC:6.1.1.7UniRule annotation)
Alternative name(s):
Alanyl-tRNA synthetaseUniRule annotation
Short name:
AlaRSUniRule annotation
Gene namesi
Name:alaSUniRule annotation
Ordered Locus Names:MG292
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Alanine--tRNA ligasePRO_0000075149Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243273.MG_292.

Structurei

3D structure databases

ProteinModelPortaliP47534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0013.
KOiK01872.
OMAiWASKGSP.
OrthoDBiEOG6Q2SQ2.

Family and domain databases

HAMAPiMF_00036_B. Ala_tRNA_synth_B.
InterProiIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR00980. TRNASYNTHALA.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00344. alaS. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47534-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNWTTDKVRQ TWLDYFAKKD HLVLASKSLI PINDPSLLWI NSGVATLKDY
60 70 80 90 100
FSARKTPPSK RLVNAQICLR VNDIENVGFT SRHQTLFEML GNFSIGDYFK
110 120 130 140 150
TEAIDFAFDL LVNYYQLDPK RFYITVYEDD ETTYKRWIKH KIDKNHIIKC
160 170 180 190 200
DKSRNFWDLG LGPCGPCTEI YYDRGEKFDP KKIGEKLFFE DIENDRYVEI
210 220 230 240 250
WNIVFSQFNN DGNGNYTELA QKNIDTGAGI ERLVSVLQNS PTNFDTDIFL
260 270 280 290 300
KLIKIIEAFC PFKYDPNSYF TFDPQKVKEQ SYFRIIADHF KAITFTISEG
310 320 330 340 350
VLPGPNERNY VVRRLLRRAL IACKKLQLNL AFIEKIIDEI IASYENYYQH
360 370 380 390 400
LKAKNETVKQ VVLKEINAFN KTIDLGLVLF EKSVKNNTLT PQLTFQLNET
410 420 430 440 450
YGFPVEIIRE LVNQKGLTID WTVFDQLMAK HRSISKQNNQ TINFEKQNIN
460 470 480 490 500
LVNFKTKSTF FYHKNKINAK VIGLFDENYL PVKELNNQSG YVVFDQTVLY
510 520 530 540 550
ATSGGQRYDE GSCINHSNNN DQKISFQGVF KGPNKQHFHY FLVGSFKLND
560 570 580 590 600
QVTLSHDETW RKLAANNHSL EHLLHAALQK EIDPLIKQSG AFKSAQKATI
610 620 630 640 650
DFNLNRHLTR NELEKVENKI RSLIKQKISS KEIFTDFEGS QKLNAIAYFE
660 670 680 690 700
EEYSQHEILR VIRFGDYSVE LCGGTHVANT ASIEDCFITD FYSLGAGRWR
710 720 730 740 750
IEIISSNETI NNYLKAENQK LIQLKSELEK VLSLIDSSIF KVELKELQQR
760 770 780 790 800
LDKFILPEKI TQLRDASDTL LALKNDINQL KTKNYKVSQQ ALALSIKKQL
810 820 830 840 850
LSLVDENKSY VIATFNDVEP KLLLQTLHDV FNQNQTKNFL IINQFNESNS
860 870 880 890 900
FIVIGNKTTT IIEKLRNSFN LKGGGNDKLF RGSFQDNVTP QKLNELFQNK
Length:900
Mass (Da):104,302
Last modified:February 1, 1996 - v1
Checksum:iAA54520BFB3949A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71513.1.
PIRiC64232.
RefSeqiNP_072959.1. NC_000908.2.
WP_010869412.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71513; AAC71513; MG_292.
GeneIDi875339.
KEGGimge:MG_292.
PATRICi20010114. VBIMycGen98045_0343.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71513.1 .
PIRi C64232.
RefSeqi NP_072959.1. NC_000908.2.
WP_010869412.1. NC_000908.2.

3D structure databases

ProteinModelPortali P47534.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243273.MG_292.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC71513 ; AAC71513 ; MG_292 .
GeneIDi 875339.
KEGGi mge:MG_292.
PATRICi 20010114. VBIMycGen98045_0343.

Phylogenomic databases

eggNOGi COG0013.
KOi K01872.
OMAi WASKGSP.
OrthoDBi EOG6Q2SQ2.

Enzyme and pathway databases

BioCyci MGEN243273:GH2R-328-MONOMER.

Family and domain databases

HAMAPi MF_00036_B. Ala_tRNA_synth_B.
InterProi IPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view ]
Pfami PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view ]
PRINTSi PR00980. TRNASYNTHALA.
SMARTi SM00863. tRNA_SAD. 1 hit.
[Graphical view ]
SUPFAMi SSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsi TIGR00344. alaS. 1 hit.
PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiSYA_MYCGE
AccessioniPrimary (citable) accession number: P47534
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 26, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3