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P47534

- SYA_MYCGE

UniProt

P47534 - SYA_MYCGE

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Protein
Alanine--tRNA ligase
Gene
alaS, MG292
Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity.UniRule annotation

Catalytic activityi

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi568 – 5681Zinc Reviewed prediction
Metal bindingi572 – 5721Zinc Reviewed prediction
Metal bindingi672 – 6721Zinc Reviewed prediction
Metal bindingi676 – 6761Zinc Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-KW
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-328-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--tRNA ligase (EC:6.1.1.7)
Alternative name(s):
Alanyl-tRNA synthetase
Short name:
AlaRS
Gene namesi
Name:alaS
Ordered Locus Names:MG292
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Alanine--tRNA ligaseUniRule annotation
PRO_0000075149Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243273.MG_292.

Structurei

3D structure databases

ProteinModelPortaliP47534.

Family & Domainsi

Domaini

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0013.
KOiK01872.
OMAiWASKGSP.
OrthoDBiEOG6Q2SQ2.

Family and domain databases

HAMAPiMF_00036_B. Ala_tRNA_synth_B.
InterProiIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR00980. TRNASYNTHALA.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00344. alaS. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47534-1 [UniParc]FASTAAdd to Basket

« Hide

MNWTTDKVRQ TWLDYFAKKD HLVLASKSLI PINDPSLLWI NSGVATLKDY    50
FSARKTPPSK RLVNAQICLR VNDIENVGFT SRHQTLFEML GNFSIGDYFK 100
TEAIDFAFDL LVNYYQLDPK RFYITVYEDD ETTYKRWIKH KIDKNHIIKC 150
DKSRNFWDLG LGPCGPCTEI YYDRGEKFDP KKIGEKLFFE DIENDRYVEI 200
WNIVFSQFNN DGNGNYTELA QKNIDTGAGI ERLVSVLQNS PTNFDTDIFL 250
KLIKIIEAFC PFKYDPNSYF TFDPQKVKEQ SYFRIIADHF KAITFTISEG 300
VLPGPNERNY VVRRLLRRAL IACKKLQLNL AFIEKIIDEI IASYENYYQH 350
LKAKNETVKQ VVLKEINAFN KTIDLGLVLF EKSVKNNTLT PQLTFQLNET 400
YGFPVEIIRE LVNQKGLTID WTVFDQLMAK HRSISKQNNQ TINFEKQNIN 450
LVNFKTKSTF FYHKNKINAK VIGLFDENYL PVKELNNQSG YVVFDQTVLY 500
ATSGGQRYDE GSCINHSNNN DQKISFQGVF KGPNKQHFHY FLVGSFKLND 550
QVTLSHDETW RKLAANNHSL EHLLHAALQK EIDPLIKQSG AFKSAQKATI 600
DFNLNRHLTR NELEKVENKI RSLIKQKISS KEIFTDFEGS QKLNAIAYFE 650
EEYSQHEILR VIRFGDYSVE LCGGTHVANT ASIEDCFITD FYSLGAGRWR 700
IEIISSNETI NNYLKAENQK LIQLKSELEK VLSLIDSSIF KVELKELQQR 750
LDKFILPEKI TQLRDASDTL LALKNDINQL KTKNYKVSQQ ALALSIKKQL 800
LSLVDENKSY VIATFNDVEP KLLLQTLHDV FNQNQTKNFL IINQFNESNS 850
FIVIGNKTTT IIEKLRNSFN LKGGGNDKLF RGSFQDNVTP QKLNELFQNK 900
Length:900
Mass (Da):104,302
Last modified:February 1, 1996 - v1
Checksum:iAA54520BFB3949A2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71513.1.
PIRiC64232.
RefSeqiNP_072959.1. NC_000908.2.
WP_010869412.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71513; AAC71513; MG_292.
GeneIDi875339.
KEGGimge:MG_292.
PATRICi20010114. VBIMycGen98045_0343.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71513.1 .
PIRi C64232.
RefSeqi NP_072959.1. NC_000908.2.
WP_010869412.1. NC_000908.2.

3D structure databases

ProteinModelPortali P47534.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243273.MG_292.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC71513 ; AAC71513 ; MG_292 .
GeneIDi 875339.
KEGGi mge:MG_292.
PATRICi 20010114. VBIMycGen98045_0343.

Phylogenomic databases

eggNOGi COG0013.
KOi K01872.
OMAi WASKGSP.
OrthoDBi EOG6Q2SQ2.

Enzyme and pathway databases

BioCyci MGEN243273:GH2R-328-MONOMER.

Family and domain databases

HAMAPi MF_00036_B. Ala_tRNA_synth_B.
InterProi IPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view ]
Pfami PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view ]
PRINTSi PR00980. TRNASYNTHALA.
SMARTi SM00863. tRNA_SAD. 1 hit.
[Graphical view ]
SUPFAMi SSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsi TIGR00344. alaS. 1 hit.
PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiSYA_MYCGE
AccessioniPrimary (citable) accession number: P47534
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 3, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi