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Protein

Proline--tRNA ligase

Gene

proS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetaseUniRule annotation
Short name:
ProRSUniRule annotation
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:MG283
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393331 – 483Proline--tRNA ligaseAdd BLAST483

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243273.MgenG_010200003285.

Structurei

3D structure databases

ProteinModelPortaliP47525.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R1M. Bacteria.
COG0442. LUCA.
KOiK01881.
OMAiWTIEAIM.
OrthoDBiPOG091H07N9.

Family and domain databases

CDDicd00778. ProRS_core_arch_euk. 1 hit.
Gene3Di3.30.110.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_01571. Pro_tRNA_synth_type3. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004499. Pro-tRNA-ligase_IIa_arc-type.
IPR016061. Pro-tRNA_ligase_II_C.
IPR017449. Pro-tRNA_synth_II.
IPR033721. ProRS_core_arch_euk.
[Graphical view]
PANTHERiPTHR11451:SF6. PTHR11451:SF6. 2 hits.
PfamiPF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SMARTiSM00946. ProRS-C_1. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF64586. SSF64586. 1 hit.
TIGRFAMsiTIGR00408. proS_fam_I. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKTQDLTS WYDQLLVKAK LICHGEVKGT VCFLNNSWGL WMEIQQLYND
60 70 80 90 100
AIANKNQLSA IALTKFQPTT SFCYQVFQVQ LPTLSFYSEY QKEKTHIKGF
110 120 130 140 150
NPELFLINQV GQKQLNDPLV LRPTSEIAFC NLWKKQELSY HDLPLIYNQW
160 170 180 190 200
TQVFRAEKNT RPFLRNSEFY WQETHGLFVD QSQSEQAAIS FWNLYQDLII
210 220 230 240 250
NKLCIPAFVG LKSESEKFAG AKNTWTIEAI MPDGQSLQCA TSHDLGDTFT
260 270 280 290 300
KSFTISYQSK TNQKMTPSSF SCGMSTRILG AIFLTHSDDY GLVLPWYLAS
310 320 330 340 350
KQVKLYLFDK NNNPKTRALA FLVKDFLEKL KIRFSFIEIN NQLGKQLLKG
360 370 380 390 400
EIEGIPLQMI VDNEKTINIF NRLTRLKTSL TFANLQTEFV NLVNNYHTEM
410 420 430 440 450
YRKANDLVEQ KLARVQTLKE IEQAFKNKKA VLCTVKLTGE LEQHLKTKYQ
460 470 480
VSVRCVFKKS DVTQNCPFTN QPCFDSVLIA RAY
Length:483
Mass (Da):55,869
Last modified:February 1, 1996 - v1
Checksum:i7CD86861298C3343
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti367I → Y (PubMed:8253680).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71505.1.
U02205 Genomic DNA. Translation: AAD12494.1.
PIRiC64231.
RefSeqiWP_009885995.1. NZ_AAGX01000018.1.

Genome annotation databases

EnsemblBacteriaiAAC71505; AAC71505; MG_283.
KEGGimge:MG_283.
PATRICi20010078. VBIMycGen98045_0326.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71505.1.
U02205 Genomic DNA. Translation: AAD12494.1.
PIRiC64231.
RefSeqiWP_009885995.1. NZ_AAGX01000018.1.

3D structure databases

ProteinModelPortaliP47525.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243273.MgenG_010200003285.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71505; AAC71505; MG_283.
KEGGimge:MG_283.
PATRICi20010078. VBIMycGen98045_0326.

Phylogenomic databases

eggNOGiENOG4107R1M. Bacteria.
COG0442. LUCA.
KOiK01881.
OMAiWTIEAIM.
OrthoDBiPOG091H07N9.

Family and domain databases

CDDicd00778. ProRS_core_arch_euk. 1 hit.
Gene3Di3.30.110.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_01571. Pro_tRNA_synth_type3. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004499. Pro-tRNA-ligase_IIa_arc-type.
IPR016061. Pro-tRNA_ligase_II_C.
IPR017449. Pro-tRNA_synth_II.
IPR033721. ProRS_core_arch_euk.
[Graphical view]
PANTHERiPTHR11451:SF6. PTHR11451:SF6. 2 hits.
PfamiPF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SMARTiSM00946. ProRS-C_1. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF64586. SSF64586. 1 hit.
TIGRFAMsiTIGR00408. proS_fam_I. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYP_MYCGE
AccessioniPrimary (citable) accession number: P47525
Secondary accession number(s): Q49322
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.