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Protein

Proline--tRNA ligase

Gene

proS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMGEN243273:G1GJ2-346-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetaseUniRule annotation
Short name:
ProRSUniRule annotation
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:MG283
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393331 – 483Proline--tRNA ligaseAdd BLAST483

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243273.MgenG_010200003285

Structurei

3D structure databases

ProteinModelPortaliP47525
SMRiP47525
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R1M Bacteria
COG0442 LUCA
KOiK01881
OMAiEVYWVTH
OrthoDBiPOG091H07N9

Family and domain databases

CDDicd00778 ProRS_core_arch_euk, 1 hit
Gene3Di3.30.110.30, 1 hit
3.40.50.800, 1 hit
HAMAPiMF_01571 Pro_tRNA_synth_type3, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR036621 Anticodon-bd_dom_sf
IPR002316 Pro-tRNA-ligase_IIa
IPR004499 Pro-tRNA-ligase_IIa_arc-type
IPR016061 Pro-tRNA_ligase_II_C
IPR017449 Pro-tRNA_synth_II
IPR033721 ProRS_core_arch_euk
PfamiView protein in Pfam
PF00587 tRNA-synt_2b, 1 hit
PRINTSiPR01046 TRNASYNTHPRO
SMARTiView protein in SMART
SM00946 ProRS-C_1, 1 hit
SUPFAMiSSF64586 SSF64586, 1 hit
TIGRFAMsiTIGR00408 proS_fam_I, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

P47525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKTQDLTS WYDQLLVKAK LICHGEVKGT VCFLNNSWGL WMEIQQLYND
60 70 80 90 100
AIANKNQLSA IALTKFQPTT SFCYQVFQVQ LPTLSFYSEY QKEKTHIKGF
110 120 130 140 150
NPELFLINQV GQKQLNDPLV LRPTSEIAFC NLWKKQELSY HDLPLIYNQW
160 170 180 190 200
TQVFRAEKNT RPFLRNSEFY WQETHGLFVD QSQSEQAAIS FWNLYQDLII
210 220 230 240 250
NKLCIPAFVG LKSESEKFAG AKNTWTIEAI MPDGQSLQCA TSHDLGDTFT
260 270 280 290 300
KSFTISYQSK TNQKMTPSSF SCGMSTRILG AIFLTHSDDY GLVLPWYLAS
310 320 330 340 350
KQVKLYLFDK NNNPKTRALA FLVKDFLEKL KIRFSFIEIN NQLGKQLLKG
360 370 380 390 400
EIEGIPLQMI VDNEKTINIF NRLTRLKTSL TFANLQTEFV NLVNNYHTEM
410 420 430 440 450
YRKANDLVEQ KLARVQTLKE IEQAFKNKKA VLCTVKLTGE LEQHLKTKYQ
460 470 480
VSVRCVFKKS DVTQNCPFTN QPCFDSVLIA RAY
Length:483
Mass (Da):55,869
Last modified:February 1, 1996 - v1
Checksum:i7CD86861298C3343
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti367I → Y (PubMed:8253680).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA Translation: AAC71505.1
U02205 Genomic DNA Translation: AAD12494.1
PIRiC64231
RefSeqiWP_009885995.1, NZ_AAGX01000018.1

Genome annotation databases

EnsemblBacteriaiAAC71505; AAC71505; MG_283
KEGGimge:MG_283

Similar proteinsi

Entry informationi

Entry nameiSYP_MYCGE
AccessioniPrimary (citable) accession number: P47525
Secondary accession number(s): Q49322
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health