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P47520 (SPOT_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

EC=3.1.7.2
Alternative name(s):
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name=(ppGpp)ase
Gene names
Name:spoT
Ordered Locus Names:MG278
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length720 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into GDP. It may also be capable of catalyzing the synthesis of ppGpp By similarity.

Catalytic activity

Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.

Cofactor

Manganese By similarity.

Pathway

Purine metabolism; ppGpp biosynthesis; ppGpp from GDP: step 1/1.

Sequence similarities

Belongs to the RelA/SpoT family.

Contains 1 HD domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 720720Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
PRO_0000166572

Regions

Domain49 – 154106HD

Experimental info

Sequence conflict219 – 2246YDNEWD → LWQWVG in AAD10588. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P47520 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 285A6E8670FD8A12

FASTA72084,139
        10         20         30         40         50         60 
MATIQEIECD FLAKIAQKFT NAEIELINKA FYHAKTWHEN QKRLSGEPFF IHPLRTALSL 

        70         80         90        100        110        120 
VEWNMDPITI CAGLLHDIIE DTDQTEANIA MIFSKEIAEL VTKVTKITNE SKKQRHLKNK 

       130        140        150        160        170        180 
KENLNLKSFV NIAINSQQEI NVMVLKLADR LDNIASIEFL PIEKQKVIAK ETLELYAKIA 

       190        200        210        220        230        240 
GRIGMYPVKT KLADLSFKVL DLKNYDNTLS KINKQKVFYD NEWDNFKQQL KKILAQNQIE 

       250        260        270        280        290        300 
YQLESRIKGI YSTYKKLTVH EQNISKIHDL FAIRLITKSE LDCYHILGLI HLNFLIDSKY 

       310        320        330        340        350        360 
FKDYIASPKQ NLYQSIHTTV RLKGLNVEIQ IRTQQMDNVS KFGLASHWIY KEQKEGLLAP 

       370        380        390        400        410        420 
ALQLNYLVTK QKHSHDFLKR IFGTDIIKIN VSASHEPNVI KQINVDSNNK LLDIAFENYP 

       430        440        450        460        470        480 
KQFAKLTKIE IDGVEINSFD TSVENEMLIE FYFGKNNNLK SKWIRYMNNP IYREKVKKSL 

       490        500        510        520        530        540 
AKLAKSGRYS ELAFYEKELG EKQLKLASET EIQKRLNTLR IKKMSDYLAL IECTNFTNDE 

       550        560        570        580        590        600 
HLLFLAKNND KWNKLTKPLK FAFSKVVFHN SYFEQIEGIF ITKIVIEPCC SKIPDMPEQV 

       610        620        630        640        650        660 
TGILTKNILS VHRYGCKNLQ NKKQLKIIPL YWNIQQLKLK PRKFRSYINI NGVWSEKTIN 

       670        680        690        700        710        720 
KICQTIINGD GYIEKIIPKI NKQKDEFDLN ITLFVNNYQQ LLTLMDQITT KNISFSWKYL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC71500.1.
U01770 Genomic DNA. Translation: AAD10588.1.
PIRG64230.
RefSeqNP_072945.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47520.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_278.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC71500; AAC71500; MG_278.
GeneID875246.
KEGGmge:MG_278.
PATRIC20010056. VBIMycGen98045_0320.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0317.
OMANIMRLYQ.
OrthoDBEOG6SV551.
ProtClustDBCLSK336950.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-307-MONOMER.
UniPathwayUPA00908; UER00886.

Family and domain databases

InterProIPR026020. (p)ppGpp_Synthase.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
[Graphical view]
PANTHERPTHR21262:SF1. PTHR21262:SF1. 1 hit.
PfamPF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
TIGRFAMsTIGR00691. spoT_relA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSPOT_MYCGE
AccessionPrimary (citable) accession number: P47520
Secondary accession number(s): Q49221
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 13, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names