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Protein

Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Gene

spoT

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into GDP. It may also be capable of catalyzing the synthesis of ppGpp (By similarity).By similarity

Catalytic activityi

Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.

Cofactori

Mn2+By similarity

Pathwayi: ppGpp biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GDP.
Proteins known to be involved in this subpathway in this organism are:
  1. Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (spoT)
This subpathway is part of the pathway ppGpp biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GDP, the pathway ppGpp biosynthesis and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Manganese

Enzyme and pathway databases

UniPathwayiUPA00908; UER00886.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC:3.1.7.2)
Alternative name(s):
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name:
(ppGpp)ase
Gene namesi
Name:spoT
Ordered Locus Names:MG278
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001665721 – 720Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolaseAdd BLAST720

Interactioni

Protein-protein interaction databases

STRINGi243273.MgenG_010200002679.

Structurei

3D structure databases

ProteinModelPortaliP47520.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 154HDAdd BLAST106

Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated
Contains 1 HD domain.Curated

Phylogenomic databases

eggNOGiENOG4105CWR. Bacteria.
COG0317. LUCA.
OMAiIIVENEF.
OrthoDBiPOG091H01YD.

Family and domain databases

CDDicd05399. NT_Rel-Spo_like. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
[Graphical view]
PfamiPF13328. HD_4. 1 hit.
PF04607. RelA_SpoT. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.

Sequencei

Sequence statusi: Complete.

P47520-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATIQEIECD FLAKIAQKFT NAEIELINKA FYHAKTWHEN QKRLSGEPFF
60 70 80 90 100
IHPLRTALSL VEWNMDPITI CAGLLHDIIE DTDQTEANIA MIFSKEIAEL
110 120 130 140 150
VTKVTKITNE SKKQRHLKNK KENLNLKSFV NIAINSQQEI NVMVLKLADR
160 170 180 190 200
LDNIASIEFL PIEKQKVIAK ETLELYAKIA GRIGMYPVKT KLADLSFKVL
210 220 230 240 250
DLKNYDNTLS KINKQKVFYD NEWDNFKQQL KKILAQNQIE YQLESRIKGI
260 270 280 290 300
YSTYKKLTVH EQNISKIHDL FAIRLITKSE LDCYHILGLI HLNFLIDSKY
310 320 330 340 350
FKDYIASPKQ NLYQSIHTTV RLKGLNVEIQ IRTQQMDNVS KFGLASHWIY
360 370 380 390 400
KEQKEGLLAP ALQLNYLVTK QKHSHDFLKR IFGTDIIKIN VSASHEPNVI
410 420 430 440 450
KQINVDSNNK LLDIAFENYP KQFAKLTKIE IDGVEINSFD TSVENEMLIE
460 470 480 490 500
FYFGKNNNLK SKWIRYMNNP IYREKVKKSL AKLAKSGRYS ELAFYEKELG
510 520 530 540 550
EKQLKLASET EIQKRLNTLR IKKMSDYLAL IECTNFTNDE HLLFLAKNND
560 570 580 590 600
KWNKLTKPLK FAFSKVVFHN SYFEQIEGIF ITKIVIEPCC SKIPDMPEQV
610 620 630 640 650
TGILTKNILS VHRYGCKNLQ NKKQLKIIPL YWNIQQLKLK PRKFRSYINI
660 670 680 690 700
NGVWSEKTIN KICQTIINGD GYIEKIIPKI NKQKDEFDLN ITLFVNNYQQ
710 720
LLTLMDQITT KNISFSWKYL
Length:720
Mass (Da):84,139
Last modified:October 1, 1996 - v1
Checksum:i285A6E8670FD8A12
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti219 – 224YDNEWD → LWQWVG in AAD10588 (PubMed:8253680).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71500.1.
U01770 Genomic DNA. Translation: AAD10588.1.
PIRiG64230.
RefSeqiWP_009885909.1. NZ_AAGX01000009.1.

Genome annotation databases

EnsemblBacteriaiAAC71500; AAC71500; MG_278.
KEGGimge:MG_278.
PATRICi20010056. VBIMycGen98045_0320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71500.1.
U01770 Genomic DNA. Translation: AAD10588.1.
PIRiG64230.
RefSeqiWP_009885909.1. NZ_AAGX01000009.1.

3D structure databases

ProteinModelPortaliP47520.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243273.MgenG_010200002679.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71500; AAC71500; MG_278.
KEGGimge:MG_278.
PATRICi20010056. VBIMycGen98045_0320.

Phylogenomic databases

eggNOGiENOG4105CWR. Bacteria.
COG0317. LUCA.
OMAiIIVENEF.
OrthoDBiPOG091H01YD.

Enzyme and pathway databases

UniPathwayiUPA00908; UER00886.

Family and domain databases

CDDicd05399. NT_Rel-Spo_like. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
[Graphical view]
PfamiPF13328. HD_4. 1 hit.
PF04607. RelA_SpoT. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSPOT_MYCGE
AccessioniPrimary (citable) accession number: P47520
Secondary accession number(s): Q49221
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.