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P47508

- SYL_MYCGE

UniProt

P47508 - SYL_MYCGE

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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei572 – 5721ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-293-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:MG266
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 792792Leucine--tRNA ligasePRO_0000152045Add
BLAST

Proteomic databases

PRIDEiP47508.

Interactioni

Protein-protein interaction databases

STRINGi243273.MG_266.

Structurei

3D structure databases

ProteinModelPortaliP47508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi39 – 5012"HIGH" regionAdd
BLAST
Motifi569 – 5735"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiHELEVYT.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47508-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYNHNLIEEK WLKKWKNKDV NRFESDSNKK KYYVLDMFPY PSAAGLHLGH
60 70 80 90 100
VRAYTITDVI SRYYKAKGFN VIHPIGFDAF GLPAEQYAIN SNQNPGSWTD
110 120 130 140 150
QNINNFINQL TSFGFDYDYH LSLKTTDPRY YKYTQWIFSE LFKANLAELV
160 170 180 190 200
DIDVNWCEQL GTVLANEEVL IDSNGNAVSE RGSFSVEKRK MKQWVLKITT
210 220 230 240 250
FADALLEGLD TLDWPEPIKE MQRNWIGKSK GVTINFQLKD HKEAIAIFTT
260 270 280 290 300
KPQTIFGVSF LAVSTNHWLA KKIAETNKKV ASFLKKQLQK TTTLKQKATL
310 320 330 340 350
YDGIDLLTNA IHPLTNELIP VYVANYVIEG YGTDAIMGVG AHNENDNFFA
360 370 380 390 400
RKQKLKIINV IDKKERLQNS FAYNGLTTKE AQVAITNELI SQNKAKLTTV
410 420 430 440 450
YKLRDWIFSR QRYWGEPFPI IFDENNTPHL VEQLPVELPL LENYKPDGSG
460 470 480 490 500
NSPLMRNQAW VNIVKDNIHY QRETNTMPQW AGSCWYYLGY LMLIKNPNFW
510 520 530 540 550
PIDSKEAKKL FDQYLPVDLY VGGAEHAVLH LLYARFWHKF LFDKKLVSTK
560 570 580 590 600
EPFQKLINQG MVLGPDGKKM SKSKGNTINP TPLVDSHGAD ALRLYLMFMG
610 620 630 640 650
PISASLTWND EGLNGMRRWL DRVYNFFFNH AVVTDQVSQE TIFAYNLFLK
660 670 680 690 700
NSYCHLDKHE LNLVISEMMI FLNFLYKTKK ISLNYAKGFL TVLSFFAPFL
710 720 730 740 750
AEELNEKCGL EPFVVKQAIS LVDYQLFETA KTKVILSING KFKAAKEFTK
760 770 780 790
GSLEIDVLES FKQDKEINDI LNQPIERVVY VQDRIINVLL KK
Length:792
Mass (Da):91,474
Last modified:February 1, 1996 - v1
Checksum:iD2146C8681E10DA5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti86 – 861Q → R in AAD10600. (PubMed:8253680)Curated
Sequence conflicti225 – 2251W → V in AAD12449. (PubMed:8253680)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71488.1.
U01780 Genomic DNA. Translation: AAD10600.1.
U02167 Genomic DNA. Translation: AAD12449.1.
PIRiD64229.
RefSeqiNP_072933.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71488; AAC71488; MG_266.
GeneIDi875347.
KEGGimge:MG_266.
PATRICi20010030. VBIMycGen98045_0307.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71488.1 .
U01780 Genomic DNA. Translation: AAD10600.1 .
U02167 Genomic DNA. Translation: AAD12449.1 .
PIRi D64229.
RefSeqi NP_072933.1. NC_000908.2.

3D structure databases

ProteinModelPortali P47508.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243273.MG_266.

Proteomic databases

PRIDEi P47508.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC71488 ; AAC71488 ; MG_266 .
GeneIDi 875347.
KEGGi mge:MG_266.
PATRICi 20010030. VBIMycGen98045_0307.

Phylogenomic databases

eggNOGi COG0495.
KOi K01869.
OMAi HELEVYT.
OrthoDBi EOG63Z74X.

Enzyme and pathway databases

BioCyci MGEN243273:GH2R-293-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_B. Leu_tRNA_synth_B.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfami PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-96 AND 208-319.
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiSYL_MYCGE
AccessioniPrimary (citable) accession number: P47508
Secondary accession number(s): Q49228, Q49290
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3