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P47508

- SYL_MYCGE

UniProt

P47508 - SYL_MYCGE

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei572 – 5721ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMGEN243273:GH2R-293-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:MG266
    OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
    Taxonomic identifieri243273 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000807: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 792792Leucine--tRNA ligasePRO_0000152045Add
    BLAST

    Proteomic databases

    PRIDEiP47508.

    Interactioni

    Protein-protein interaction databases

    STRINGi243273.MG_266.

    Structurei

    3D structure databases

    ProteinModelPortaliP47508.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi39 – 5012"HIGH" regionAdd
    BLAST
    Motifi569 – 5735"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    KOiK01869.
    OMAiHELEVYT.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47508-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYNHNLIEEK WLKKWKNKDV NRFESDSNKK KYYVLDMFPY PSAAGLHLGH    50
    VRAYTITDVI SRYYKAKGFN VIHPIGFDAF GLPAEQYAIN SNQNPGSWTD 100
    QNINNFINQL TSFGFDYDYH LSLKTTDPRY YKYTQWIFSE LFKANLAELV 150
    DIDVNWCEQL GTVLANEEVL IDSNGNAVSE RGSFSVEKRK MKQWVLKITT 200
    FADALLEGLD TLDWPEPIKE MQRNWIGKSK GVTINFQLKD HKEAIAIFTT 250
    KPQTIFGVSF LAVSTNHWLA KKIAETNKKV ASFLKKQLQK TTTLKQKATL 300
    YDGIDLLTNA IHPLTNELIP VYVANYVIEG YGTDAIMGVG AHNENDNFFA 350
    RKQKLKIINV IDKKERLQNS FAYNGLTTKE AQVAITNELI SQNKAKLTTV 400
    YKLRDWIFSR QRYWGEPFPI IFDENNTPHL VEQLPVELPL LENYKPDGSG 450
    NSPLMRNQAW VNIVKDNIHY QRETNTMPQW AGSCWYYLGY LMLIKNPNFW 500
    PIDSKEAKKL FDQYLPVDLY VGGAEHAVLH LLYARFWHKF LFDKKLVSTK 550
    EPFQKLINQG MVLGPDGKKM SKSKGNTINP TPLVDSHGAD ALRLYLMFMG 600
    PISASLTWND EGLNGMRRWL DRVYNFFFNH AVVTDQVSQE TIFAYNLFLK 650
    NSYCHLDKHE LNLVISEMMI FLNFLYKTKK ISLNYAKGFL TVLSFFAPFL 700
    AEELNEKCGL EPFVVKQAIS LVDYQLFETA KTKVILSING KFKAAKEFTK 750
    GSLEIDVLES FKQDKEINDI LNQPIERVVY VQDRIINVLL KK 792
    Length:792
    Mass (Da):91,474
    Last modified:February 1, 1996 - v1
    Checksum:iD2146C8681E10DA5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti86 – 861Q → R in AAD10600. (PubMed:8253680)Curated
    Sequence conflicti225 – 2251W → V in AAD12449. (PubMed:8253680)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71488.1.
    U01780 Genomic DNA. Translation: AAD10600.1.
    U02167 Genomic DNA. Translation: AAD12449.1.
    PIRiD64229.
    RefSeqiNP_072933.1. NC_000908.2.
    WP_010869401.1. NC_000908.2.

    Genome annotation databases

    EnsemblBacteriaiAAC71488; AAC71488; MG_266.
    GeneIDi875347.
    KEGGimge:MG_266.
    PATRICi20010030. VBIMycGen98045_0307.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71488.1 .
    U01780 Genomic DNA. Translation: AAD10600.1 .
    U02167 Genomic DNA. Translation: AAD12449.1 .
    PIRi D64229.
    RefSeqi NP_072933.1. NC_000908.2.
    WP_010869401.1. NC_000908.2.

    3D structure databases

    ProteinModelPortali P47508.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243273.MG_266.

    Proteomic databases

    PRIDEi P47508.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC71488 ; AAC71488 ; MG_266 .
    GeneIDi 875347.
    KEGGi mge:MG_266.
    PATRICi 20010030. VBIMycGen98045_0307.

    Phylogenomic databases

    eggNOGi COG0495.
    KOi K01869.
    OMAi HELEVYT.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci MGEN243273:GH2R-293-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33530 / G-37 / NCTC 10195.
    2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
      Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
      J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-96 AND 208-319.
      Strain: ATCC 33530 / G-37 / NCTC 10195.

    Entry informationi

    Entry nameiSYL_MYCGE
    AccessioniPrimary (citable) accession number: P47508
    Secondary accession number(s): Q49228, Q49290
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Mycoplasma genitalium
      Mycoplasma genitalium (strain G-37): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3