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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei572ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:MG266
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001520451 – 792Leucine--tRNA ligaseAdd BLAST792

Proteomic databases

PRIDEiP47508.

Interactioni

Protein-protein interaction databases

STRINGi243273.MgenG_010200002609.

Structurei

3D structure databases

ProteinModelPortaliP47508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi39 – 50"HIGH" regionAdd BLAST12
Motifi569 – 573"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
KOiK01869.
OMAiPRKLCAH.
OrthoDBiPOG091H01JZ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 4 hits.
PfamiPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47508-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNHNLIEEK WLKKWKNKDV NRFESDSNKK KYYVLDMFPY PSAAGLHLGH
60 70 80 90 100
VRAYTITDVI SRYYKAKGFN VIHPIGFDAF GLPAEQYAIN SNQNPGSWTD
110 120 130 140 150
QNINNFINQL TSFGFDYDYH LSLKTTDPRY YKYTQWIFSE LFKANLAELV
160 170 180 190 200
DIDVNWCEQL GTVLANEEVL IDSNGNAVSE RGSFSVEKRK MKQWVLKITT
210 220 230 240 250
FADALLEGLD TLDWPEPIKE MQRNWIGKSK GVTINFQLKD HKEAIAIFTT
260 270 280 290 300
KPQTIFGVSF LAVSTNHWLA KKIAETNKKV ASFLKKQLQK TTTLKQKATL
310 320 330 340 350
YDGIDLLTNA IHPLTNELIP VYVANYVIEG YGTDAIMGVG AHNENDNFFA
360 370 380 390 400
RKQKLKIINV IDKKERLQNS FAYNGLTTKE AQVAITNELI SQNKAKLTTV
410 420 430 440 450
YKLRDWIFSR QRYWGEPFPI IFDENNTPHL VEQLPVELPL LENYKPDGSG
460 470 480 490 500
NSPLMRNQAW VNIVKDNIHY QRETNTMPQW AGSCWYYLGY LMLIKNPNFW
510 520 530 540 550
PIDSKEAKKL FDQYLPVDLY VGGAEHAVLH LLYARFWHKF LFDKKLVSTK
560 570 580 590 600
EPFQKLINQG MVLGPDGKKM SKSKGNTINP TPLVDSHGAD ALRLYLMFMG
610 620 630 640 650
PISASLTWND EGLNGMRRWL DRVYNFFFNH AVVTDQVSQE TIFAYNLFLK
660 670 680 690 700
NSYCHLDKHE LNLVISEMMI FLNFLYKTKK ISLNYAKGFL TVLSFFAPFL
710 720 730 740 750
AEELNEKCGL EPFVVKQAIS LVDYQLFETA KTKVILSING KFKAAKEFTK
760 770 780 790
GSLEIDVLES FKQDKEINDI LNQPIERVVY VQDRIINVLL KK
Length:792
Mass (Da):91,474
Last modified:February 1, 1996 - v1
Checksum:iD2146C8681E10DA5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86Q → R in AAD10600 (PubMed:8253680).Curated1
Sequence conflicti225W → V in AAD12449 (PubMed:8253680).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71488.1.
U01780 Genomic DNA. Translation: AAD10600.1.
U02167 Genomic DNA. Translation: AAD12449.1.
PIRiD64229.
RefSeqiWP_010869401.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71488; AAC71488; MG_266.
KEGGimge:MG_266.
PATRICi20010030. VBIMycGen98045_0307.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71488.1.
U01780 Genomic DNA. Translation: AAD10600.1.
U02167 Genomic DNA. Translation: AAD12449.1.
PIRiD64229.
RefSeqiWP_010869401.1. NC_000908.2.

3D structure databases

ProteinModelPortaliP47508.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243273.MgenG_010200002609.

Proteomic databases

PRIDEiP47508.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71488; AAC71488; MG_266.
KEGGimge:MG_266.
PATRICi20010030. VBIMycGen98045_0307.

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
KOiK01869.
OMAiPRKLCAH.
OrthoDBiPOG091H01JZ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 4 hits.
PfamiPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYL_MYCGE
AccessioniPrimary (citable) accession number: P47508
Secondary accession number(s): Q49228, Q49290
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.