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P47508 (SYL_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:MG266
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length792 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 792792Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152045

Regions

Motif39 – 5012"HIGH" region HAMAP-Rule MF_00049
Motif569 – 5735"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5721ATP By similarity

Experimental info

Sequence conflict861Q → R in AAD10600. Ref.2
Sequence conflict2251W → V in AAD12449. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P47508 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: D2146C8681E10DA5

FASTA79291,474
        10         20         30         40         50         60 
MYNHNLIEEK WLKKWKNKDV NRFESDSNKK KYYVLDMFPY PSAAGLHLGH VRAYTITDVI 

        70         80         90        100        110        120 
SRYYKAKGFN VIHPIGFDAF GLPAEQYAIN SNQNPGSWTD QNINNFINQL TSFGFDYDYH 

       130        140        150        160        170        180 
LSLKTTDPRY YKYTQWIFSE LFKANLAELV DIDVNWCEQL GTVLANEEVL IDSNGNAVSE 

       190        200        210        220        230        240 
RGSFSVEKRK MKQWVLKITT FADALLEGLD TLDWPEPIKE MQRNWIGKSK GVTINFQLKD 

       250        260        270        280        290        300 
HKEAIAIFTT KPQTIFGVSF LAVSTNHWLA KKIAETNKKV ASFLKKQLQK TTTLKQKATL 

       310        320        330        340        350        360 
YDGIDLLTNA IHPLTNELIP VYVANYVIEG YGTDAIMGVG AHNENDNFFA RKQKLKIINV 

       370        380        390        400        410        420 
IDKKERLQNS FAYNGLTTKE AQVAITNELI SQNKAKLTTV YKLRDWIFSR QRYWGEPFPI 

       430        440        450        460        470        480 
IFDENNTPHL VEQLPVELPL LENYKPDGSG NSPLMRNQAW VNIVKDNIHY QRETNTMPQW 

       490        500        510        520        530        540 
AGSCWYYLGY LMLIKNPNFW PIDSKEAKKL FDQYLPVDLY VGGAEHAVLH LLYARFWHKF 

       550        560        570        580        590        600 
LFDKKLVSTK EPFQKLINQG MVLGPDGKKM SKSKGNTINP TPLVDSHGAD ALRLYLMFMG 

       610        620        630        640        650        660 
PISASLTWND EGLNGMRRWL DRVYNFFFNH AVVTDQVSQE TIFAYNLFLK NSYCHLDKHE 

       670        680        690        700        710        720 
LNLVISEMMI FLNFLYKTKK ISLNYAKGFL TVLSFFAPFL AEELNEKCGL EPFVVKQAIS 

       730        740        750        760        770        780 
LVDYQLFETA KTKVILSING KFKAAKEFTK GSLEIDVLES FKQDKEINDI LNQPIERVVY 

       790 
VQDRIINVLL KK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC71488.1.
U01780 Genomic DNA. Translation: AAD10600.1.
U02167 Genomic DNA. Translation: AAD12449.1.
PIRD64229.
RefSeqNP_072933.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47508.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_266.

Proteomic databases

PRIDEP47508.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC71488; AAC71488; MG_266.
GeneID875347.
KEGGmge:MG_266.
PATRIC20010030. VBIMycGen98045_0307.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAHELEVYT.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-293-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_MYCGE
AccessionPrimary (citable) accession number: P47508
Secondary accession number(s): Q49228, Q49290
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries