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Protein

Peptide chain release factor 1

Gene

prfA

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.By similarity

GO - Molecular functioni

  1. translation release factor activity, codon specific Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-283-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide chain release factor 1
Short name:
RF-1
Gene namesi
Name:prfA
Ordered Locus Names:MG258
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Peptide chain release factor 1PRO_0000177702Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei236 – 2361N5-methylglutamineBy similarity

Post-translational modificationi

Methylated by PrmC. Methylation increases the termination efficiency of RF1 (By similarity).By similarity

Keywords - PTMi

Methylation

Interactioni

Protein-protein interaction databases

STRINGi243273.MG_258.

Structurei

3D structure databases

ProteinModelPortaliP47500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0216.
KOiK02835.
OMAiWKVEIVS.
OrthoDBiEOG6TN48J.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
HAMAPiMF_00093. Rel_fac_1.
InterProiIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
IPR004373. PrfA.
[Graphical view]
PfamiPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTiSM00937. PCRF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00019. prfA. 1 hit.
PROSITEiPS00745. RF_PROK_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47500-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFDKQLFFN VEKIVELTEQ LEKDLNKPNL SFEQIKVINK ELKHKQPLIV
60 70 80 90 100
KFKELQKLVE NANEAEQILN NSSLKELHEE AKKELEKIKA SLPSLEEEIK
110 120 130 140 150
FLLLPVDENN QKNVIVEIRP AAGGDESCIF LSDLFNMYKN YCTSKNWTVE
160 170 180 190 200
LNEIIPASVG INFVSFAVNG TDVFAKLKFE SGVHRVQRVP LTEAKGRVHT
210 220 230 240 250
STVTVAVLPQ LEEVEITINP SDLRIDTYRA SGAGGQHVNR TESAVRITHL
260 270 280 290 300
PTGIVVACQE GKSQFSNRDK AMKMLRAKLW ENAQNKQLST QADLRKSQVG
310 320 330 340 350
SGERAEKIRT YNYPQNRITD HRIKLTINKL NTVILGDLDE IIEALQADEK

KQQLEKFIS
Length:359
Mass (Da):40,808
Last modified:February 1, 1996 - v1
Checksum:i3DBF7D8E40E43A7C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti350 – 3534KKQQ → RTQL (PubMed:8253680).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71478.1.
U01800 Genomic DNA. Translation: AAD12326.1.
PIRiE64228.
RefSeqiNP_072924.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71478; AAC71478; MG_258.
GeneIDi875351.
KEGGimge:MG_258.
PATRICi20010004. VBIMycGen98045_0295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71478.1.
U01800 Genomic DNA. Translation: AAD12326.1.
PIRiE64228.
RefSeqiNP_072924.1. NC_000908.2.

3D structure databases

ProteinModelPortaliP47500.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243273.MG_258.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71478; AAC71478; MG_258.
GeneIDi875351.
KEGGimge:MG_258.
PATRICi20010004. VBIMycGen98045_0295.

Phylogenomic databases

eggNOGiCOG0216.
KOiK02835.
OMAiWKVEIVS.
OrthoDBiEOG6TN48J.

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-283-MONOMER.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
HAMAPiMF_00093. Rel_fac_1.
InterProiIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
IPR004373. PrfA.
[Graphical view]
PfamiPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTiSM00937. PCRF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00019. prfA. 1 hit.
PROSITEiPS00745. RF_PROK_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 241-353.
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiRF1_MYCGE
AccessioniPrimary (citable) accession number: P47500
Secondary accession number(s): Q49455
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: January 7, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.