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Protein

DNA primase

Gene

dnaG

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Zn2+UniRule annotationNote: Binds 1 zinc ion per monomer.UniRule annotation
  • Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi273 – 2731Magnesium 1; catalyticUniRule annotation
Metal bindingi319 – 3191Magnesium 1; catalyticUniRule annotation
Metal bindingi319 – 3191Magnesium 2UniRule annotation
Metal bindingi321 – 3211Magnesium 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri39 – 6325CHC2-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA primase activity Source: InterPro
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication, Transcription

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-272-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primaseUniRule annotation (EC:2.7.7.-UniRule annotation)
Gene namesi
Name:dnaGUniRule annotation
Synonyms:dnaE
Ordered Locus Names:MG250
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. primosome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 607607DNA primasePRO_0000180501Add
BLAST

Interactioni

Subunit structurei

Monomer. Interacts with DnaB.UniRule annotation

Protein-protein interaction databases

STRINGi243273.MG_250.

Structurei

3D structure databases

ProteinModelPortaliP47492.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini267 – 35084ToprimUniRule annotationAdd
BLAST

Domaini

Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.UniRule annotation

Sequence similaritiesi

Belongs to the DnaG primase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri39 – 6325CHC2-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG0358.
KOiK02316.
OMAiYKDWDEL.
OrthoDBiEOG6XDGTR.

Family and domain databases

Gene3Di3.90.580.10. 1 hit.
3.90.980.10. 1 hit.
HAMAPiMF_00974. DNA_primase_DnaG.
InterProiIPR013264. DNA_primase_core_N.
IPR006295. DNA_primase_DnaG.
IPR006171. Toprim_domain.
IPR002694. Znf_CHC2.
[Graphical view]
PfamiPF01751. Toprim. 1 hit.
PF08275. Toprim_N. 1 hit.
PF01807. zf-CHC2. 1 hit.
[Graphical view]
SMARTiSM00493. TOPRIM. 1 hit.
SM00400. ZnF_CHCC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01391. dnaG. 1 hit.
PROSITEiPS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47492-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVNKSNSLDE LLKQIKITEI IQHYGVKIQT KGNSLLALCP FHDDKNPSMS
60 70 80 90 100
ISSSKNIFKC WACNAAGNGI AFIQKHDQLD WKTALKKAIE ICGIKLENWN
110 120 130 140 150
SNLLTKVDPK QKRYWEINNA LITYYQTRLK RETNPNGMNY LVEKRKLNKT
160 170 180 190 200
LIEQFQLGLA FHNEDKYLCE SMERYPFINP KIKPSELYLF SKTNQQGLGF
210 220 230 240 250
FDFNTKKATF QNQIMIPIHD FNGNPVGFSA RSVDNINKLK YKNSADHEFF
260 270 280 290 300
KKGELLFNFH RLNKNLNQLF IVEGYFDVFT LTNSKFEAVA LMGLALNDVQ
310 320 330 340 350
IKAIKAHFKE LQTLVLALDN DASGQNAVFS LIEKLNNNNF IVEIVQWEHN
360 370 380 390 400
YKDWDELYLN KGSEQVILQA NKRQNLIEYL VSFFKKQQLD QRVITNKIIA
410 420 430 440 450
FLTKNQTILN DHSFLIFLIK NLVKLLEYSD EKTLYETVLK HKEKLVSKFD
460 470 480 490 500
NNRFYINTSG HAQPPQELQK TTAALVQTAF EEAVNELWKP EIFAFALIDK
510 520 530 540 550
RFLVELKQSH LDEVFKECNF NLFDVELFIE KARIYWSENQ TANWVGFESV
560 570 580 590 600
LDQNYLLNNK ARLLEIKDIF LDELTCYQAN DFQNYLKTFQ TLLKQQKQRL

KNLKLTL
Length:607
Mass (Da):71,062
Last modified:February 1, 1996 - v1
Checksum:iA84730CBFA86BDEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71470.1.
U02146 Genomic DNA. Translation: AAD12426.1.
U01771 Genomic DNA. Translation: AAD10589.1.
PIRiF64227.
RefSeqiNP_072915.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71470; AAC71470; MG_250.
GeneIDi875320.
KEGGimge:MG_250.
PATRICi20009982. VBIMycGen98045_0287.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71470.1.
U02146 Genomic DNA. Translation: AAD12426.1.
U01771 Genomic DNA. Translation: AAD10589.1.
PIRiF64227.
RefSeqiNP_072915.1. NC_000908.2.

3D structure databases

ProteinModelPortaliP47492.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243273.MG_250.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71470; AAC71470; MG_250.
GeneIDi875320.
KEGGimge:MG_250.
PATRICi20009982. VBIMycGen98045_0287.

Phylogenomic databases

eggNOGiCOG0358.
KOiK02316.
OMAiYKDWDEL.
OrthoDBiEOG6XDGTR.

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-272-MONOMER.

Family and domain databases

Gene3Di3.90.580.10. 1 hit.
3.90.980.10. 1 hit.
HAMAPiMF_00974. DNA_primase_DnaG.
InterProiIPR013264. DNA_primase_core_N.
IPR006295. DNA_primase_DnaG.
IPR006171. Toprim_domain.
IPR002694. Znf_CHC2.
[Graphical view]
PfamiPF01751. Toprim. 1 hit.
PF08275. Toprim_N. 1 hit.
PF01807. zf-CHC2. 1 hit.
[Graphical view]
SMARTiSM00493. TOPRIM. 1 hit.
SM00400. ZnF_CHCC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01391. dnaG. 1 hit.
PROSITEiPS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 78-216 AND 511-607.
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiDNAG_MYCGE
AccessioniPrimary (citable) accession number: P47492
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: January 7, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.