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P47492 (DNAG_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA primase

EC=2.7.7.-
Gene names
Name:dnaG
Synonyms:dnaE
Ordered Locus Names:MG250
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length607 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication By similarity. HAMAP-Rule MF_00974

Cofactor

Binds 1 zinc ion per monomer By similarity. HAMAP-Rule MF_00974

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00974

Subunit structure

Monomer By similarity. Interacts with DnaB By similarity. HAMAP-Rule MF_00974

Domain

Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain By similarity. HAMAP-Rule MF_00974

Sequence similarities

Belongs to the DnaG primase family.

Contains 1 Toprim domain.

Ontologies

Keywords
   Biological processDNA replication
Transcription
   Cellular componentDNA-directed RNA polymerase
Primosome
   DomainZinc-finger
   LigandDNA-binding
Magnesium
Metal-binding
Zinc
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentprimosome complex

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA primase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 607607DNA primase HAMAP-Rule MF_00974
PRO_0000180501

Regions

Domain267 – 35084Toprim
Zinc finger39 – 6325CHC2-type By similarity

Sites

Metal binding2731Magnesium 1; catalytic By similarity
Metal binding3191Magnesium 1; catalytic By similarity
Metal binding3191Magnesium 2 By similarity
Metal binding3211Magnesium 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P47492 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: A84730CBFA86BDEA

FASTA60771,062
        10         20         30         40         50         60 
MVNKSNSLDE LLKQIKITEI IQHYGVKIQT KGNSLLALCP FHDDKNPSMS ISSSKNIFKC 

        70         80         90        100        110        120 
WACNAAGNGI AFIQKHDQLD WKTALKKAIE ICGIKLENWN SNLLTKVDPK QKRYWEINNA 

       130        140        150        160        170        180 
LITYYQTRLK RETNPNGMNY LVEKRKLNKT LIEQFQLGLA FHNEDKYLCE SMERYPFINP 

       190        200        210        220        230        240 
KIKPSELYLF SKTNQQGLGF FDFNTKKATF QNQIMIPIHD FNGNPVGFSA RSVDNINKLK 

       250        260        270        280        290        300 
YKNSADHEFF KKGELLFNFH RLNKNLNQLF IVEGYFDVFT LTNSKFEAVA LMGLALNDVQ 

       310        320        330        340        350        360 
IKAIKAHFKE LQTLVLALDN DASGQNAVFS LIEKLNNNNF IVEIVQWEHN YKDWDELYLN 

       370        380        390        400        410        420 
KGSEQVILQA NKRQNLIEYL VSFFKKQQLD QRVITNKIIA FLTKNQTILN DHSFLIFLIK 

       430        440        450        460        470        480 
NLVKLLEYSD EKTLYETVLK HKEKLVSKFD NNRFYINTSG HAQPPQELQK TTAALVQTAF 

       490        500        510        520        530        540 
EEAVNELWKP EIFAFALIDK RFLVELKQSH LDEVFKECNF NLFDVELFIE KARIYWSENQ 

       550        560        570        580        590        600 
TANWVGFESV LDQNYLLNNK ARLLEIKDIF LDELTCYQAN DFQNYLKTFQ TLLKQQKQRL 


KNLKLTL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC71470.1.
U02146 Genomic DNA. Translation: AAD12426.1.
U01771 Genomic DNA. Translation: AAD10589.1.
PIRF64227.
RefSeqNP_072915.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47492.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_250.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC71470; AAC71470; MG_250.
GeneID875320.
KEGGmge:MG_250.
PATRIC20009982. VBIMycGen98045_0287.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0358.
KOK02316.
OMAYKDWDEL.
OrthoDBEOG6XDGTR.
ProtClustDBCLSK335064.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-272-MONOMER.

Family and domain databases

Gene3D3.90.580.10. 1 hit.
3.90.980.10. 1 hit.
HAMAPMF_00974. DNA_primase_DnaG.
InterProIPR013264. DNA_primase_core_N.
IPR006295. DNA_primase_DnaG.
IPR006171. Toprim_domain.
IPR002694. Znf_CHC2.
[Graphical view]
PfamPF01751. Toprim. 1 hit.
PF08275. Toprim_N. 1 hit.
PF01807. zf-CHC2. 1 hit.
[Graphical view]
SMARTSM00493. TOPRIM. 1 hit.
SM00400. ZnF_CHCC. 1 hit.
[Graphical view]
TIGRFAMsTIGR01391. dnaG. 1 hit.
PROSITEPS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNAG_MYCGE
AccessionPrimary (citable) accession number: P47492
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 19, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names