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P47492

- DNAG_MYCGE

UniProt

P47492 - DNAG_MYCGE

Protein

DNA primase

Gene

dnaG

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.UniRule annotation

    Cofactori

    Binds 1 zinc ion per monomer.UniRule annotation
    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi273 – 2731Magnesium 1; catalyticUniRule annotation
    Metal bindingi319 – 3191Magnesium 1; catalyticUniRule annotation
    Metal bindingi319 – 3191Magnesium 2UniRule annotation
    Metal bindingi321 – 3211Magnesium 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri39 – 6325CHC2-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA primase activity Source: InterPro
    3. zinc ion binding Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication, Transcription

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMGEN243273:GH2R-272-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA primaseUniRule annotation (EC:2.7.7.-UniRule annotation)
    Gene namesi
    Name:dnaGUniRule annotation
    Synonyms:dnaE
    Ordered Locus Names:MG250
    OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
    Taxonomic identifieri243273 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000807: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. primosome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Primosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 607607DNA primasePRO_0000180501Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer. Interacts with DnaB.UniRule annotation

    Protein-protein interaction databases

    STRINGi243273.MG_250.

    Structurei

    3D structure databases

    ProteinModelPortaliP47492.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini267 – 35084ToprimUniRule annotationAdd
    BLAST

    Domaini

    Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.UniRule annotation

    Sequence similaritiesi

    Belongs to the DnaG primase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri39 – 6325CHC2-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0358.
    KOiK02316.
    OMAiCESMERY.
    OrthoDBiEOG6XDGTR.

    Family and domain databases

    Gene3Di3.90.580.10. 1 hit.
    3.90.980.10. 1 hit.
    HAMAPiMF_00974. DNA_primase_DnaG.
    InterProiIPR013264. DNA_primase_core_N.
    IPR006295. DNA_primase_DnaG.
    IPR006171. Toprim_domain.
    IPR002694. Znf_CHC2.
    [Graphical view]
    PfamiPF01751. Toprim. 1 hit.
    PF08275. Toprim_N. 1 hit.
    PF01807. zf-CHC2. 1 hit.
    [Graphical view]
    SMARTiSM00493. TOPRIM. 1 hit.
    SM00400. ZnF_CHCC. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01391. dnaG. 1 hit.
    PROSITEiPS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47492-1 [UniParc]FASTAAdd to Basket

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    MVNKSNSLDE LLKQIKITEI IQHYGVKIQT KGNSLLALCP FHDDKNPSMS    50
    ISSSKNIFKC WACNAAGNGI AFIQKHDQLD WKTALKKAIE ICGIKLENWN 100
    SNLLTKVDPK QKRYWEINNA LITYYQTRLK RETNPNGMNY LVEKRKLNKT 150
    LIEQFQLGLA FHNEDKYLCE SMERYPFINP KIKPSELYLF SKTNQQGLGF 200
    FDFNTKKATF QNQIMIPIHD FNGNPVGFSA RSVDNINKLK YKNSADHEFF 250
    KKGELLFNFH RLNKNLNQLF IVEGYFDVFT LTNSKFEAVA LMGLALNDVQ 300
    IKAIKAHFKE LQTLVLALDN DASGQNAVFS LIEKLNNNNF IVEIVQWEHN 350
    YKDWDELYLN KGSEQVILQA NKRQNLIEYL VSFFKKQQLD QRVITNKIIA 400
    FLTKNQTILN DHSFLIFLIK NLVKLLEYSD EKTLYETVLK HKEKLVSKFD 450
    NNRFYINTSG HAQPPQELQK TTAALVQTAF EEAVNELWKP EIFAFALIDK 500
    RFLVELKQSH LDEVFKECNF NLFDVELFIE KARIYWSENQ TANWVGFESV 550
    LDQNYLLNNK ARLLEIKDIF LDELTCYQAN DFQNYLKTFQ TLLKQQKQRL 600
    KNLKLTL 607
    Length:607
    Mass (Da):71,062
    Last modified:February 1, 1996 - v1
    Checksum:iA84730CBFA86BDEA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71470.1.
    U02146 Genomic DNA. Translation: AAD12426.1.
    U01771 Genomic DNA. Translation: AAD10589.1.
    PIRiF64227.
    RefSeqiNP_072915.1. NC_000908.2.

    Genome annotation databases

    EnsemblBacteriaiAAC71470; AAC71470; MG_250.
    GeneIDi875320.
    KEGGimge:MG_250.
    PATRICi20009982. VBIMycGen98045_0287.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71470.1 .
    U02146 Genomic DNA. Translation: AAD12426.1 .
    U01771 Genomic DNA. Translation: AAD10589.1 .
    PIRi F64227.
    RefSeqi NP_072915.1. NC_000908.2.

    3D structure databases

    ProteinModelPortali P47492.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243273.MG_250.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC71470 ; AAC71470 ; MG_250 .
    GeneIDi 875320.
    KEGGi mge:MG_250.
    PATRICi 20009982. VBIMycGen98045_0287.

    Phylogenomic databases

    eggNOGi COG0358.
    KOi K02316.
    OMAi CESMERY.
    OrthoDBi EOG6XDGTR.

    Enzyme and pathway databases

    BioCyci MGEN243273:GH2R-272-MONOMER.

    Family and domain databases

    Gene3Di 3.90.580.10. 1 hit.
    3.90.980.10. 1 hit.
    HAMAPi MF_00974. DNA_primase_DnaG.
    InterProi IPR013264. DNA_primase_core_N.
    IPR006295. DNA_primase_DnaG.
    IPR006171. Toprim_domain.
    IPR002694. Znf_CHC2.
    [Graphical view ]
    Pfami PF01751. Toprim. 1 hit.
    PF08275. Toprim_N. 1 hit.
    PF01807. zf-CHC2. 1 hit.
    [Graphical view ]
    SMARTi SM00493. TOPRIM. 1 hit.
    SM00400. ZnF_CHCC. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01391. dnaG. 1 hit.
    PROSITEi PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33530 / G-37 / NCTC 10195.
    2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
      Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
      J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 78-216 AND 511-607.
      Strain: ATCC 33530 / G-37 / NCTC 10195.

    Entry informationi

    Entry nameiDNAG_MYCGE
    AccessioniPrimary (citable) accession number: P47492
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Mycoplasma genitalium
      Mycoplasma genitalium (strain G-37): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3