P47492 (PRIM_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA primase
EC=2.7.7.-

Gene names

Name:	dnaG
Synonyms:	dnaE
Ordered Locus Names:	MG250

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	607 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis.
Cofactor	Binds 1 zinc ion per monomer By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the DNA primase family. Contains 1 Toprim domain.

Ontologies

Keywords
Biological process	DNA replication Transcription
Cellular component	DNA-directed RNA polymerase Primosome
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	replisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA primase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 607	607	DNA primase		PRO_0000180501
Regions
	Domain	267 – 350	84	Toprim
	Zinc finger	39 – 63	25	CHC2-type By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47492 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: A84730CBFA86BDEA	FASTA	607	71,062
	10 20 30 40 50 60 MVNKSNSLDE LLKQIKITEI IQHYGVKIQT KGNSLLALCP FHDDKNPSMS ISSSKNIFKC 70 80 90 100 110 120 WACNAAGNGI AFIQKHDQLD WKTALKKAIE ICGIKLENWN SNLLTKVDPK QKRYWEINNA 130 140 150 160 170 180 LITYYQTRLK RETNPNGMNY LVEKRKLNKT LIEQFQLGLA FHNEDKYLCE SMERYPFINP 190 200 210 220 230 240 KIKPSELYLF SKTNQQGLGF FDFNTKKATF QNQIMIPIHD FNGNPVGFSA RSVDNINKLK 250 260 270 280 290 300 YKNSADHEFF KKGELLFNFH RLNKNLNQLF IVEGYFDVFT LTNSKFEAVA LMGLALNDVQ 310 320 330 340 350 360 IKAIKAHFKE LQTLVLALDN DASGQNAVFS LIEKLNNNNF IVEIVQWEHN YKDWDELYLN 370 380 390 400 410 420 KGSEQVILQA NKRQNLIEYL VSFFKKQQLD QRVITNKIIA FLTKNQTILN DHSFLIFLIK 430 440 450 460 470 480 NLVKLLEYSD EKTLYETVLK HKEKLVSKFD NNRFYINTSG HAQPPQELQK TTAALVQTAF 490 500 510 520 530 540 EEAVNELWKP EIFAFALIDK RFLVELKQSH LDEVFKECNF NLFDVELFIE KARIYWSENQ 550 560 570 580 590 600 TANWVGFESV LDQNYLLNNK ARLLEIKDIF LDELTCYQAN DFQNYLKTFQ TLLKQQKQRL KNLKLTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 78-216 AND 511-607. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71470.1. U02146 Genomic DNA. Translation: AAD12426.1. U01771 Genomic DNA. Translation: AAD10589.1.
PIR	F64227.
RefSeq	NP_072915.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47492.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_250.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71470; AAC71470; MG_250.
GeneID	875320.
KEGG	mge:MG_250.
PATRIC	20009982. VBIMycGen98045_0287.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0358.
KO	K02316.
OMA	YKDWDEL.
ProtClustDB	CLSK335064.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-297-MONOMER.
Family and domain databases
Gene3D	3.90.580.10. 1 hit. 3.90.980.10. 1 hit.
InterPro	IPR013264. DNA_primase_core_N. IPR006295. DNA_primase_DnaG. IPR006171. Toprim_domain. IPR002694. Znf_CHC2. [Graphical view]
Pfam	PF01751. Toprim. 1 hit. PF08275. Toprim_N. 1 hit. PF01807. zf-CHC2. 1 hit. [Graphical view]
SMART	SM00493. TOPRIM. 1 hit. SM00400. ZnF_CHCC. 1 hit. [Graphical view]
TIGRFAMs	TIGR01391. dnaG. 1 hit.
PROSITE	PS50880. TOPRIM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRIM_MYCGE

Accession

Primary (citable) accession number: P47492

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program