Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

5-formyltetrahydrofolate cyclo-ligase

Gene

MG245

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in folate metabolism. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate (By similarity).By similarity

Catalytic activityi

ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511Substrate; via carbonyl oxygenBy similarity
Binding sitei56 – 561SubstrateBy similarity
Metal bindingi125 – 1251MagnesiumBy similarity
Binding sitei126 – 1261ATPBy similarity
Binding sitei154 – 1541ATPBy similarity
Metal bindingi155 – 1551MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi4 – 85ATPBy similarity
Nucleotide bindingi116 – 1249ATPBy similarity

GO - Molecular functioni

  1. 5-formyltetrahydrofolate cyclo-ligase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-267-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
5-formyltetrahydrofolate cyclo-ligase (EC:6.3.3.2)
Alternative name(s):
5,10-methenyl-tetrahydrofolate synthetase
Short name:
MTHFS
Short name:
Methenyl-THF synthetase
Gene namesi
Ordered Locus Names:MG245
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1651655-formyltetrahydrofolate cyclo-ligasePRO_0000200287Add
BLAST

Interactioni

Subunit structurei

Monomer or homodimer.By similarity

Protein-protein interaction databases

STRINGi243273.MG_245.

Structurei

3D structure databases

ProteinModelPortaliP47487.
SMRiP47487. Positions 2-162.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0212.
KOiK01934.
OMAiTEIISAW.
OrthoDBiEOG6RVG1P.

Family and domain databases

Gene3Di3.40.50.10420. 1 hit.
InterProiIPR002698. FTHF_cligase.
IPR024185. FTHF_cligase-like.
[Graphical view]
PANTHERiPTHR23407:SF1. PTHR23407:SF1. 1 hit.
PfamiPF01812. 5-FTHF_cyc-lig. 1 hit.
[Graphical view]
PIRSFiPIRSF006806. FTHF_cligase. 1 hit.

Sequencei

Sequence statusi: Complete.

P47487-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVDKNSLRKL MLLKRAELND LEKSHLDQKI NQKLMAFLIT RPTIKNLALY
60 70 80 90 100
IPIKNEVAFL DNFLDFLKLN KITSCFPSIV DQFNMKFIDQ NNNEINPNDI
110 120 130 140 150
DCFFIPLLAF NKANHRIGFG KGYYDRYLSL TSKKQLKIGI AYDFQYAEFT
160
NDPWDYQLDL IICNG
Length:165
Mass (Da):19,355
Last modified:October 1, 1996 - v1
Checksum:iAEC4ADEEE55A7020
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71465.1.
PIRiA64227.
RefSeqiNP_072910.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71465; AAC71465; MG_245.
GeneIDi875369.
KEGGimge:MG_245.
PATRICi20009972. VBIMycGen98045_0282.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71465.1.
PIRiA64227.
RefSeqiNP_072910.1. NC_000908.2.

3D structure databases

ProteinModelPortaliP47487.
SMRiP47487. Positions 2-162.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243273.MG_245.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71465; AAC71465; MG_245.
GeneIDi875369.
KEGGimge:MG_245.
PATRICi20009972. VBIMycGen98045_0282.

Phylogenomic databases

eggNOGiCOG0212.
KOiK01934.
OMAiTEIISAW.
OrthoDBiEOG6RVG1P.

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-267-MONOMER.

Family and domain databases

Gene3Di3.40.50.10420. 1 hit.
InterProiIPR002698. FTHF_cligase.
IPR024185. FTHF_cligase-like.
[Graphical view]
PANTHERiPTHR23407:SF1. PTHR23407:SF1. 1 hit.
PfamiPF01812. 5-FTHF_cyc-lig. 1 hit.
[Graphical view]
PIRSFiPIRSF006806. FTHF_cligase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiMTHFS_MYCGE
AccessioniPrimary (citable) accession number: P47487
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.