Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lon protease

Gene

lon

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.UniRule annotation

Catalytic activityi

Hydrolysis of proteins in presence of ATP.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei702UniRule annotation1
Active sitei745UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi379 – 386ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

MEROPSiS16.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Lon proteaseUniRule annotation (EC:3.4.21.53UniRule annotation)
Alternative name(s):
ATP-dependent protease LaUniRule annotation
Gene namesi
Name:lonUniRule annotation
Ordered Locus Names:MG239
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000761391 – 795Lon proteaseAdd BLAST795

Expressioni

Inductioni

By heat shock.UniRule annotation

Interactioni

Subunit structurei

Homohexamer. Organized in a ring with a central cavity.UniRule annotation

Protein-protein interaction databases

STRINGi243273.MgenG_010200001622.

Structurei

3D structure databases

ProteinModelPortaliP47481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 211Lon N-terminalPROSITE-ProRule annotationAdd BLAST205
Domaini615 – 795Lon proteolyticPROSITE-ProRule annotationAdd BLAST181

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi304 – 308Poly-Ser5

Sequence similaritiesi

Belongs to the peptidase S16 family.UniRule annotation
Contains 1 Lon N-terminal domain.PROSITE-ProRule annotation
Contains 1 Lon proteolytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C6P. Bacteria.
COG0466. LUCA.
KOiK01338.
OMAiEGTFMPG.
OrthoDBiPOG091H00WA.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01973. lon_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027543. Lon_bac.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR003111. LON_substr-bd_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF05362. Lon_C. 1 hit.
PF02190. LON_substr_bdg. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS51787. LON_N. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
PS01046. LON_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47481-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVTKKSQIL VVRGQVIFPF VPFSLDVGRP RSRKIIKALK TLKTKRLVLV
60 70 80 90 100
TQKFTGEQNP EFNDIYHVGT LCEIDEIVDV PGVDSKTVDY RIKGRGLQRV
110 120 130 140 150
LIEKFSDADI NEVSYQLLNS TVKDEANVDR FLQRIFPEKE EIEQLMEGAE
160 170 180 190 200
KFLELENISK TVNVPKGLKQ LDIITFKLAN LVPNTESIKQ AILEENEIAN
210 220 230 240 250
RLEKIIQAGI EDLQKIQDYG RSKNKETEFD KLDSKITRKI NEQLSRQQRD
260 270 280 290 300
FYLREKLRII REEIGISSKK EDEVASIRKK LDENPYPEAI KKRILSELEH
310 320 330 340 350
YENSSSSSQE STLTKTYIDT LLNLPWWQKS KDNSDVKNLI KTLDKNHTGL
360 370 380 390 400
DKVKERIVEY LAVQLRTQKN KGPIMCLVGP PGVGKSSLAK SIAEALDKKF
410 420 430 440 450
VKISLGGVHD ESEIRGHRKT YLGSMPGRIL KGMTRAKVIN PLFLLDEIDK
460 470 480 490 500
MTSSNQGYPS GALLEVLDPE LNNKFSDNYV EEDYDLSKVM FIATANYIED
510 520 530 540 550
IPEALLDRME IIELTSYTEQ EKIEIAKNHL IKRCLEDADL NSEELKFTDE
560 570 580 590 600
AISYIIKFYT REAGVRQLER LIQQVVRKYI VAMQKDGIKQ ETIDVNAVKK
610 620 630 640 650
YLKKEIFDHT MRDEVSLPGI VNGMAYTPTG GDLLPIEVTH VAGKGELILT
660 670 680 690 700
GNLKQTMRES ANVALGYVKA NAERFNINPS LFKKIDINIH VPGGGIPKDG
710 720 730 740 750
PSAGAALVTA IISSLTGKKV DPTVAMTGEI TLRGKVLVIG GVKEKTISAY
760 770 780 790
RGGVTTIFMP EKNERYLDEV PKEIVDKLNI IFVKEYSDIY NKLFS
Length:795
Mass (Da):89,988
Last modified:February 1, 1996 - v1
Checksum:i31C8188EA40F5E39
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti494T → S in AAD12428 (PubMed:8253680).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71460.1.
U02148 Genomic DNA. Translation: AAD12428.1.
PIRiD64226.
RefSeqiWP_009885776.1. NZ_AAGX01000005.1.

Genome annotation databases

EnsemblBacteriaiAAC71460; AAC71460; MG_239.
KEGGimge:MG_239.
PATRICi20009960. VBIMycGen98045_0276.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71460.1.
U02148 Genomic DNA. Translation: AAD12428.1.
PIRiD64226.
RefSeqiWP_009885776.1. NZ_AAGX01000005.1.

3D structure databases

ProteinModelPortaliP47481.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243273.MgenG_010200001622.

Protein family/group databases

MEROPSiS16.004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71460; AAC71460; MG_239.
KEGGimge:MG_239.
PATRICi20009960. VBIMycGen98045_0276.

Phylogenomic databases

eggNOGiENOG4105C6P. Bacteria.
COG0466. LUCA.
KOiK01338.
OMAiEGTFMPG.
OrthoDBiPOG091H00WA.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01973. lon_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027543. Lon_bac.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR003111. LON_substr-bd_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF05362. Lon_C. 1 hit.
PF02190. LON_substr_bdg. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS51787. LON_N. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
PS01046. LON_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLON_MYCGE
AccessioniPrimary (citable) accession number: P47481
Secondary accession number(s): Q49276
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.