Reviewed, UniProtKB/Swiss-Prot P47481 (LON_MYCGE)

Names and origin

Protein names

Recommended name:
    ATP-dependent protease La
    EC=3.4.21.53

Gene names

Name:	lon
Ordered Locus Names:	MG239

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	795 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Degrades short-lived regulatory and abnormal proteins in presence of ATP. Degrades the regulatory proteins rcsA and sulA. Hydrolyzes two ATPs for each peptide bond cleaved in the protein substrate By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 795	795	ATP-dependent protease La		PRO_0000076139
Regions
	Domain	8 – 207	200	Lon
	Nucleotide binding	379 – 386	8	ATP Potential
	Compositional bias	304 – 308	5	Poly-Ser
Sites
	Active site	702	1	By similarity
	Active site	745	1	By similarity
Experimental info
	Sequence conflict	494	1	T → S in AAD12428. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47481-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 31C8188EA40F5E39	FASTA	795	89,988
	10 20 30 40 50 60 MPVTKKSQIL VVRGQVIFPF VPFSLDVGRP RSRKIIKALK TLKTKRLVLV TQKFTGEQNP 70 80 90 100 110 120 EFNDIYHVGT LCEIDEIVDV PGVDSKTVDY RIKGRGLQRV LIEKFSDADI NEVSYQLLNS 130 140 150 160 170 180 TVKDEANVDR FLQRIFPEKE EIEQLMEGAE KFLELENISK TVNVPKGLKQ LDIITFKLAN 190 200 210 220 230 240 LVPNTESIKQ AILEENEIAN RLEKIIQAGI EDLQKIQDYG RSKNKETEFD KLDSKITRKI 250 260 270 280 290 300 NEQLSRQQRD FYLREKLRII REEIGISSKK EDEVASIRKK LDENPYPEAI KKRILSELEH 310 320 330 340 350 360 YENSSSSSQE STLTKTYIDT LLNLPWWQKS KDNSDVKNLI KTLDKNHTGL DKVKERIVEY 370 380 390 400 410 420 LAVQLRTQKN KGPIMCLVGP PGVGKSSLAK SIAEALDKKF VKISLGGVHD ESEIRGHRKT 430 440 450 460 470 480 YLGSMPGRIL KGMTRAKVIN PLFLLDEIDK MTSSNQGYPS GALLEVLDPE LNNKFSDNYV 490 500 510 520 530 540 EEDYDLSKVM FIATANYIED IPEALLDRME IIELTSYTEQ EKIEIAKNHL IKRCLEDADL 550 560 570 580 590 600 NSEELKFTDE AISYIIKFYT REAGVRQLER LIQQVVRKYI VAMQKDGIKQ ETIDVNAVKK 610 620 630 640 650 660 YLKKEIFDHT MRDEVSLPGI VNGMAYTPTG GDLLPIEVTH VAGKGELILT GNLKQTMRES 670 680 690 700 710 720 ANVALGYVKA NAERFNINPS LFKKIDINIH VPGGGIPKDG PSAGAALVTA IISSLTGKKV 730 740 750 760 770 780 DPTVAMTGEI TLRGKVLVIG GVKEKTISAY RGGVTTIFMP EKNERYLDEV PKEIVDKLNI 790 IFVKEYSDIY NKLFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 484-606. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43967 Genomic DNA. Translation: AAC71460.1. U02148 Genomic DNA. Translation: AAD12428.1.
PIR	D64226.
RefSeq	NP_072905.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S16.004.
Genome annotation databases
GeneID	875303.
GenomeReviews	Gene locus MG239 in contig L43967_GR.
KEGG	mge:MG_239.
TIGR	MG239.
Phylogenomic databases
HOGENOM	P47481.
OMA	P47481. HTLISII.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_239-MON.
BRENDA	3.4.21.53. 110.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR001984. Peptidase_S16_C. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PRINTS	PR00830. ENDOLAPTASE.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
TIGRFAMs	TIGR00763. lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_MYCGE

Accession

Primary (citable) accession number: P47481
Secondary accession number(s): Q49276

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)