Trigger factor - Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)

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P47480 (TIG_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 94. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Trigger factor
Short name=TF
EC=5.2.1.8

Alternative name(s):
PPIase

Gene names

Name:	tig
Ordered Locus Names:	MG238

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma ›

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase By similarity. HAMAP-Rule MF_00303
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0). HAMAP-Rule MF_00303
Subcellular location	Cytoplasm. Note: About half TF is bound to the ribosome near the polypeptide exit tunnel while the other half is free in the cytoplasm By similarity. HAMAP-Rule MF_00303
Domain	Consists of 3 domains; the N-terminus binds the ribosome, the middle domain has PPIase activity, while the C-terminus has intrinsic chaperone activity on its own By similarity. HAMAP-Rule MF_00303
Sequence similarities	Belongs to the FKBP-type PPIase family. Tig subfamily. Contains 1 PPIase FKBP-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm
Molecular function	Chaperone Isomerase Rotamase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW protein folding Inferred from electronic annotation. Source: HAMAP protein peptidyl-prolyl isomerization Inferred from electronic annotation. Source: GOC protein transport Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	peptidyl-prolyl cis-trans isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 444

444

Trigger factor HAMAP-Rule MF_00303

PRO_0000179382

Regions

Domain

170 – 255

PPIase FKBP-type

Secondary structure

444

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P47480 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 99704A2EA4E23F47
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FASTA

444

50,931

        10         20         30         40         50         60 
MKLYKVLNSK TTDKSLCLEV EIDPNYWQAT QKKLVGEMAK SIKIKGFRPG KIPPNLASQS 

        70         80         90        100        110        120 
INKAELMQKS AQNVMNSIYE SVQQEEIVAS NDNVIDDYPT IDFKTITEQN CVLLFYFDLI 

       130        140        150        160        170        180 
PNFQLPDYKK IKDLTPLTKL TEAEFNNEIE KLAKTKSTMV DVSDKKLANG DIAIIDFTGI 

       190        200        210        220        230        240 
VDNKKLASAS AQNYELTIGS NSFIKGFETG LIAMKVNQKK TLALTFPSDY HVKELQSKPV 

       250        260        270        280        290        300 
TFEVVLKAIK KLEFTPMDET NFKSFLPEQF QSFTSLKAFK SYFHKLMENK KQETILQENN 

       310        320        330        340        350        360 
QKIRQFLLTN TKLPFLPEAL IKLEANRLLK LQQSQAEQYK IPFEKLLSAS NITLTELQDR 

       370        380        390        400        410        420 
NIKEAKENVT FALVMKKIAD IEKIKVDNNK IKAEIENVIA VEYPFASDEM KKQLFFNMEQ 

       430        440 
QKEFVESIII NRLTTTKIVS YSTH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 141-216. Strain: ATCC 33530 / G-37 / NCTC 10195.
[3]	"NMR solution structure and dynamics of the peptidyl-prolyl cis-trans isomerase domain of the trigger factor from Mycoplasma genitalium compared to FK506-binding protein." Vogtherr M., Jacobs D.M., Parac T.N., Maurer M., Pahl A., Saxena K., Rueterjans H., Griesinger C., Fiebig K.M. J. Mol. Biol. 318:1097-1115(2002) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 166-250.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L43967 Genomic DNA. Translation: AAC71459.1.
U01772 Genomic DNA. Translation: AAD10591.1.

PIR

C64226.

RefSeq

NP_072904.1. NC_000908.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HXV	NMR	-	A	150-250	[»]

ProteinModelPortal

P47480.

SMR

P47480. Positions 166-250.

ModBase

Search...

Protein-protein interaction databases

STRING

243273.MG_238.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC71459; AAC71459; MG_238.

GeneID

875304.

KEGG

mge:MG_238.

PATRIC

20009958. VBIMycGen98045_0275.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0544.

K03545.

OMA

LVHEELM.

ProtClustDB

CLSK335216.

Enzyme and pathway databases

BioCyc

MGEN243273:GH2R-285-MONOMER.

Family and domain databases

Gene3D

1.10.3120.10. 1 hit.
3.30.70.1050. 1 hit.

HAMAP

MF_00303. Trigger_factor_Tig.

InterPro

IPR001179. PPIase_FKBP_dom.
IPR005215. Trig_fac.
IPR008880. Trigger_fac_C.
IPR008881. Trigger_fac_ribosome-bd_bac.
IPR027304. Trigger_fact/SurA_dom.
[Graphical view]

Pfam

PF00254. FKBP_C. 1 hit.
PF05698. Trigger_C. 1 hit.
PF05697. Trigger_N. 1 hit.
[Graphical view]

PIRSF

PIRSF003095. Trigger_factor. 1 hit.

SUPFAM

SSF109998. Trigger_fac_C_bac. 1 hit.
SSF102735. Trigger_fac_ribosome-bd_bac. 1 hit.

TIGRFAMs

TIGR00115. tig. 1 hit.

PROSITE

PS50059. FKBP_PPIASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P47480.

Entry information

Entry name

TIG_MYCGE

Accession

Primary (citable) accession number: P47480

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents