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Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene

nrdE

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei159SubstrateBy similarity1
Sitei176Important for hydrogen atom transferBy similarity1
Sitei183Allosteric effector bindingBy similarity1
Binding sitei204Substrate; via amide nitrogenBy similarity1
Sitei213Allosteric effector bindingBy similarity1
Active sitei384Proton acceptorBy similarity1
Active sitei386Cysteine radical intermediateBy similarity1
Active sitei388Proton acceptorBy similarity1
Sitei413Important for hydrogen atom transferBy similarity1
Sitei694Important for electron transferBy similarity1
Sitei695Important for electron transferBy similarity1
Sitei716Interacts with thioredoxin/glutaredoxinBy similarity1
Sitei719Interacts with thioredoxin/glutaredoxinBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdE
Ordered Locus Names:MG231
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872171 – 721Ribonucleoside-diphosphate reductase subunit alphaAdd BLAST721

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi176 ↔ 413Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

STRINGi243273.MgenG_010200001582.

Structurei

3D structure databases

ProteinModelPortaliP47473.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni175 – 176Substrate bindingBy similarity2
Regioni384 – 388Substrate bindingBy similarity5
Regioni589 – 593Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105BZH. Bacteria.
COG0209. LUCA.
KOiK00525.
OMAiTLFMTDK.
OrthoDBiPOG091H03NG.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR026459. RNR_1b_NrdE.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR013554. RNR_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PF08343. RNR_N. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
TIGR04170. RNR_1b_NrdE. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47473-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKEKIPTF NTEEDVESYI SFNAQAKIYD DFAIDLQAVE SYIQEHVKPK
60 70 80 90 100
TKVFHSTKER LDFLIKNDYY DEKIINMYSF EQFEEITHKA YSYRFRYANF
110 120 130 140 150
MGAFKFYNAY ALKTFDGKYY LENYEDRVVM NVLMLANGNF NKALKLLKQI
160 170 180 190 200
ILNRFQPATP TFLNAGRKKR GEFVSCYLLR IEDNMESIGR AITTTLQLSK
210 220 230 240 250
RDGGVALLLS NLREAGAPIK KIENQSSGII PIMKLLEDSF SYSNQLGQRQ
260 270 280 290 300
GAGAVYLHCH HPDVMQFLDT KRENADEKIR IKSLSLGLVI PDITFQLAKN
310 320 330 340 350
NEMMALFSPY DIYQEYGKAL SDISVTEMYY ELLENQRIKK TFISARKFFQ
360 370 380 390 400
TIAELHFESG YPYILFDDTV NRRNAHKNRI VMSNLCSEIV QPSLPSEFYS
410 420 430 440 450
DLTFKKVGSD ISCNLGSLNI ARAMESGSEL AELIQLAIES LDLVSRISSL
460 470 480 490 500
ETAPSIKKGN SENHALGLGA MNLHGFLATN AIYYDSKEAV DFTNIFFYTV
510 520 530 540 550
AYHAFSASNK LALELGKFKD FENTKFADGS YFDKYTKVAS DFWTCKTEKV
560 570 580 590 600
QALFDKYQVK IPTQENWKQL VASIQKDGLA NSHLMAIAPT GSISYLSSCT
610 620 630 640 650
PSLQPVVSPV EVRKEGKLGR IYVPAYKLDN DNYQYFKDGA YELGFEPIIN
660 670 680 690 700
IVAAAQQHVD QAISLTLFMT DKATTRDLNK AYIYAFKKGC SSIYYVRVRQ
710 720
DVLKDSEDHT IKIKDCEVCS I
Length:721
Mass (Da):82,249
Last modified:February 1, 1996 - v1
Checksum:i75E0174979CCAA59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71452.1.
PIRiE64225.
RefSeqiWP_009885768.1. NZ_AAGX01000005.1.

Genome annotation databases

EnsemblBacteriaiAAC71452; AAC71452; MG_231.
KEGGimge:MG_231.
PATRICi20009944. VBIMycGen98045_0268.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71452.1.
PIRiE64225.
RefSeqiWP_009885768.1. NZ_AAGX01000005.1.

3D structure databases

ProteinModelPortaliP47473.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243273.MgenG_010200001582.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71452; AAC71452; MG_231.
KEGGimge:MG_231.
PATRICi20009944. VBIMycGen98045_0268.

Phylogenomic databases

eggNOGiENOG4105BZH. Bacteria.
COG0209. LUCA.
KOiK00525.
OMAiTLFMTDK.
OrthoDBiPOG091H03NG.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR026459. RNR_1b_NrdE.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR013554. RNR_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PF08343. RNR_N. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
TIGR04170. RNR_1b_NrdE. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_MYCGE
AccessioniPrimary (citable) accession number: P47473
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.