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P47473

- RIR1_MYCGE

UniProt

P47473 - RIR1_MYCGE

Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene

nrdE

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Enzyme regulationi

    Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei159 – 1591SubstrateBy similarity
    Sitei176 – 1761Important for hydrogen atom transferBy similarity
    Sitei183 – 1831Allosteric effector bindingBy similarity
    Binding sitei204 – 2041Substrate; via amide nitrogenBy similarity
    Sitei213 – 2131Allosteric effector bindingBy similarity
    Active sitei384 – 3841Proton acceptorBy similarity
    Active sitei386 – 3861Cysteine radical intermediateBy similarity
    Active sitei388 – 3881Proton acceptorBy similarity
    Sitei413 – 4131Important for hydrogen atom transferBy similarity
    Sitei694 – 6941Important for electron transferBy similarity
    Sitei695 – 6951Important for electron transferBy similarity
    Sitei716 – 7161Interacts with thioredoxin/glutaredoxinBy similarity
    Sitei719 – 7191Interacts with thioredoxin/glutaredoxinBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMGEN243273:GH2R-253-MONOMER.
    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase
    Gene namesi
    Name:nrdE
    Ordered Locus Names:MG231
    OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
    Taxonomic identifieri243273 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000807: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. ribonucleoside-diphosphate reductase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 721721Ribonucleoside-diphosphate reductase subunit alphaPRO_0000187217Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi176 ↔ 413Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.By similarity

    Protein-protein interaction databases

    STRINGi243273.MG_231.

    Structurei

    3D structure databases

    ProteinModelPortaliP47473.
    SMRiP47473. Positions 18-700.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni175 – 1762Substrate bindingBy similarity
    Regioni384 – 3885Substrate bindingBy similarity
    Regioni589 – 5935Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0209.
    KOiK00525.
    OMAiVSEASEY.
    OrthoDBiEOG6J48HC.

    Family and domain databases

    InterProiIPR013346. NrdE_NrdA.
    IPR026459. RNR_1b_NrdE.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    IPR013554. RNR_N.
    IPR008926. RNR_R1-su_N.
    [Graphical view]
    PfamiPF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    PF08343. RNR_N. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    SUPFAMiSSF48168. SSF48168. 1 hit.
    TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
    TIGR04170. RNR_1b_NrdE. 1 hit.
    PROSITEiPS00089. RIBORED_LARGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47473-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSKEKIPTF NTEEDVESYI SFNAQAKIYD DFAIDLQAVE SYIQEHVKPK    50
    TKVFHSTKER LDFLIKNDYY DEKIINMYSF EQFEEITHKA YSYRFRYANF 100
    MGAFKFYNAY ALKTFDGKYY LENYEDRVVM NVLMLANGNF NKALKLLKQI 150
    ILNRFQPATP TFLNAGRKKR GEFVSCYLLR IEDNMESIGR AITTTLQLSK 200
    RDGGVALLLS NLREAGAPIK KIENQSSGII PIMKLLEDSF SYSNQLGQRQ 250
    GAGAVYLHCH HPDVMQFLDT KRENADEKIR IKSLSLGLVI PDITFQLAKN 300
    NEMMALFSPY DIYQEYGKAL SDISVTEMYY ELLENQRIKK TFISARKFFQ 350
    TIAELHFESG YPYILFDDTV NRRNAHKNRI VMSNLCSEIV QPSLPSEFYS 400
    DLTFKKVGSD ISCNLGSLNI ARAMESGSEL AELIQLAIES LDLVSRISSL 450
    ETAPSIKKGN SENHALGLGA MNLHGFLATN AIYYDSKEAV DFTNIFFYTV 500
    AYHAFSASNK LALELGKFKD FENTKFADGS YFDKYTKVAS DFWTCKTEKV 550
    QALFDKYQVK IPTQENWKQL VASIQKDGLA NSHLMAIAPT GSISYLSSCT 600
    PSLQPVVSPV EVRKEGKLGR IYVPAYKLDN DNYQYFKDGA YELGFEPIIN 650
    IVAAAQQHVD QAISLTLFMT DKATTRDLNK AYIYAFKKGC SSIYYVRVRQ 700
    DVLKDSEDHT IKIKDCEVCS I 721
    Length:721
    Mass (Da):82,249
    Last modified:February 1, 1996 - v1
    Checksum:i75E0174979CCAA59
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71452.1.
    PIRiE64225.
    RefSeqiNP_072897.1. NC_000908.2.

    Genome annotation databases

    EnsemblBacteriaiAAC71452; AAC71452; MG_231.
    GeneIDi875308.
    KEGGimge:MG_231.
    PATRICi20009944. VBIMycGen98045_0268.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71452.1 .
    PIRi E64225.
    RefSeqi NP_072897.1. NC_000908.2.

    3D structure databases

    ProteinModelPortali P47473.
    SMRi P47473. Positions 18-700.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243273.MG_231.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC71452 ; AAC71452 ; MG_231 .
    GeneIDi 875308.
    KEGGi mge:MG_231.
    PATRICi 20009944. VBIMycGen98045_0268.

    Phylogenomic databases

    eggNOGi COG0209.
    KOi K00525.
    OMAi VSEASEY.
    OrthoDBi EOG6J48HC.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .
    BioCyci MGEN243273:GH2R-253-MONOMER.

    Family and domain databases

    InterProi IPR013346. NrdE_NrdA.
    IPR026459. RNR_1b_NrdE.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    IPR013554. RNR_N.
    IPR008926. RNR_R1-su_N.
    [Graphical view ]
    Pfami PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    PF08343. RNR_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    SUPFAMi SSF48168. SSF48168. 1 hit.
    TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
    TIGR04170. RNR_1b_NrdE. 1 hit.
    PROSITEi PS00089. RIBORED_LARGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33530 / G-37 / NCTC 10195.

    Entry informationi

    Entry nameiRIR1_MYCGE
    AccessioniPrimary (citable) accession number: P47473
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome

    Documents

    1. Mycoplasma genitalium
      Mycoplasma genitalium (strain G-37): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3