Reviewed, UniProtKB/Swiss-Prot P47473 (RIR1_MYCGE)

Names and origin

Protein names

Recommended name:
    Ribonucleoside-diphosphate reductase subunit alpha
    EC=1.17.4.1
Alternative name(s):
    Ribonucleotide reductase

Gene names

Name:	nrdE
Ordered Locus Names:	MG231

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	721 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 721	721	Ribonucleoside-diphosphate reductase subunit alpha		PRO_0000187217
Sites
	Active site	176	1	Hydrogen atom transfer By similarity
	Active site	384	1	Proton acceptor By similarity
	Active site	386	1	Proton acceptor By similarity
	Active site	388	1	Proton acceptor By similarity
	Active site	413	1	Hydrogen atom transfer By similarity
	Active site	694	1	Electron transfer By similarity
	Active site	695	1	Electron transfer By similarity
	Site	183	1	Allosteric effector binding By similarity
	Site	213	1	Allosteric effector binding By similarity
	Site	716	1	Interacts with thioredoxin/glutaredoxin By similarity
	Site	719	1	Interacts with thioredoxin/glutaredoxin By similarity
Amino acid modifications
	Disulfide bond	176 ↔ 413		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47473-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 75E0174979CCAA59	FASTA	721	82,249
	10 20 30 40 50 60 MTSKEKIPTF NTEEDVESYI SFNAQAKIYD DFAIDLQAVE SYIQEHVKPK TKVFHSTKER 70 80 90 100 110 120 LDFLIKNDYY DEKIINMYSF EQFEEITHKA YSYRFRYANF MGAFKFYNAY ALKTFDGKYY 130 140 150 160 170 180 LENYEDRVVM NVLMLANGNF NKALKLLKQI ILNRFQPATP TFLNAGRKKR GEFVSCYLLR 190 200 210 220 230 240 IEDNMESIGR AITTTLQLSK RDGGVALLLS NLREAGAPIK KIENQSSGII PIMKLLEDSF 250 260 270 280 290 300 SYSNQLGQRQ GAGAVYLHCH HPDVMQFLDT KRENADEKIR IKSLSLGLVI PDITFQLAKN 310 320 330 340 350 360 NEMMALFSPY DIYQEYGKAL SDISVTEMYY ELLENQRIKK TFISARKFFQ TIAELHFESG 370 380 390 400 410 420 YPYILFDDTV NRRNAHKNRI VMSNLCSEIV QPSLPSEFYS DLTFKKVGSD ISCNLGSLNI 430 440 450 460 470 480 ARAMESGSEL AELIQLAIES LDLVSRISSL ETAPSIKKGN SENHALGLGA MNLHGFLATN 490 500 510 520 530 540 AIYYDSKEAV DFTNIFFYTV AYHAFSASNK LALELGKFKD FENTKFADGS YFDKYTKVAS 550 560 570 580 590 600 DFWTCKTEKV QALFDKYQVK IPTQENWKQL VASIQKDGLA NSHLMAIAPT GSISYLSSCT 610 620 630 640 650 660 PSLQPVVSPV EVRKEGKLGR IYVPAYKLDN DNYQYFKDGA YELGFEPIIN IVAAAQQHVD 670 680 690 700 710 720 QAISLTLFMT DKATTRDLNK AYIYAFKKGC SSIYYVRVRQ DVLKDSEDHT IKIKDCEVCS I

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43967 Genomic DNA. Translation: AAC71452.1.
PIR	E64225.
RefSeq	NP_072897.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	875308.
GenomeReviews	Gene locus MG231 in contig L43967_GR.
KEGG	mge:MG_231.
TIGR	MG231.
Phylogenomic databases
HOGENOM	P47473.
OMA	HIPTQDD.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_231-MON.
BRENDA	1.17.4.1. 110.
Family and domain databases
InterPro	IPR013346. NrdE_NrdA. IPR013509. Ribncl_Rdtase_lsu_N. IPR000788. Ribncl_red_lg_C. IPR013554. Ribonucl_Rdtase_N. [Graphical view]
PANTHER	PTHR11573. Ribncl_red_lg_C. 1 hit.
Pfam	PF02867. Ribonuc_red_lgC. 1 hit. PF00317. Ribonuc_red_lgN. 1 hit. PF08343. RNR_N. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02506. NrdE_NrdA. 1 hit.
PROSITE	PS00089. RIBORED_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR1_MYCGE

Accession

Primary (citable) accession number: P47473

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)