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P47473 (RIR1_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase subunit alpha

EC=1.17.4.1
Alternative name(s):
Ribonucleotide reductase
Gene names
Name:nrdE
Ordered Locus Names:MG231
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length721 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulation

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Tetramer of two alpha and two beta subunits By similarity.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase large chain family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 721721Ribonucleoside-diphosphate reductase subunit alpha
PRO_0000187217

Regions

Region175 – 1762Substrate binding By similarity
Region384 – 3885Substrate binding By similarity
Region589 – 5935Substrate binding By similarity

Sites

Active site3841Proton acceptor By similarity
Active site3861Cysteine radical intermediate By similarity
Active site3881Proton acceptor By similarity
Binding site1591Substrate By similarity
Binding site2041Substrate; via amide nitrogen By similarity
Site1761Important for hydrogen atom transfer By similarity
Site1831Allosteric effector binding By similarity
Site2131Allosteric effector binding By similarity
Site4131Important for hydrogen atom transfer By similarity
Site6941Important for electron transfer By similarity
Site6951Important for electron transfer By similarity
Site7161Interacts with thioredoxin/glutaredoxin By similarity
Site7191Interacts with thioredoxin/glutaredoxin By similarity

Amino acid modifications

Disulfide bond176 ↔ 413Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
P47473 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 75E0174979CCAA59

FASTA72182,249
        10         20         30         40         50         60 
MTSKEKIPTF NTEEDVESYI SFNAQAKIYD DFAIDLQAVE SYIQEHVKPK TKVFHSTKER 

        70         80         90        100        110        120 
LDFLIKNDYY DEKIINMYSF EQFEEITHKA YSYRFRYANF MGAFKFYNAY ALKTFDGKYY 

       130        140        150        160        170        180 
LENYEDRVVM NVLMLANGNF NKALKLLKQI ILNRFQPATP TFLNAGRKKR GEFVSCYLLR 

       190        200        210        220        230        240 
IEDNMESIGR AITTTLQLSK RDGGVALLLS NLREAGAPIK KIENQSSGII PIMKLLEDSF 

       250        260        270        280        290        300 
SYSNQLGQRQ GAGAVYLHCH HPDVMQFLDT KRENADEKIR IKSLSLGLVI PDITFQLAKN 

       310        320        330        340        350        360 
NEMMALFSPY DIYQEYGKAL SDISVTEMYY ELLENQRIKK TFISARKFFQ TIAELHFESG 

       370        380        390        400        410        420 
YPYILFDDTV NRRNAHKNRI VMSNLCSEIV QPSLPSEFYS DLTFKKVGSD ISCNLGSLNI 

       430        440        450        460        470        480 
ARAMESGSEL AELIQLAIES LDLVSRISSL ETAPSIKKGN SENHALGLGA MNLHGFLATN 

       490        500        510        520        530        540 
AIYYDSKEAV DFTNIFFYTV AYHAFSASNK LALELGKFKD FENTKFADGS YFDKYTKVAS 

       550        560        570        580        590        600 
DFWTCKTEKV QALFDKYQVK IPTQENWKQL VASIQKDGLA NSHLMAIAPT GSISYLSSCT 

       610        620        630        640        650        660 
PSLQPVVSPV EVRKEGKLGR IYVPAYKLDN DNYQYFKDGA YELGFEPIIN IVAAAQQHVD 

       670        680        690        700        710        720 
QAISLTLFMT DKATTRDLNK AYIYAFKKGC SSIYYVRVRQ DVLKDSEDHT IKIKDCEVCS 


I 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC71452.1.
PIRE64225.
RefSeqNP_072897.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47473.
SMRP47473. Positions 18-700.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_231.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC71452; AAC71452; MG_231.
GeneID875308.
KEGGmge:MG_231.
PATRIC20009944. VBIMycGen98045_0268.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0209.
KOK00525.
OMAIHPIVSR.
OrthoDBEOG6J48HC.
ProtClustDBPRK07187.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-253-MONOMER.
UniPathwayUPA00326.

Family and domain databases

InterProIPR013346. NrdE_NrdA.
IPR026459. RNR_1b_NrdE.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR013554. RNR_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PF08343. RNR_N. 1 hit.
[Graphical view]
PRINTSPR01183. RIBORDTASEM1.
SUPFAMSSF48168. SSF48168. 1 hit.
TIGRFAMsTIGR02506. NrdE_NrdA. 1 hit.
TIGR04170. RNR_1b_NrdE. 1 hit.
PROSITEPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIR1_MYCGE
AccessionPrimary (citable) accession number: P47473
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 13, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names