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P47473

- RIR1_MYCGE

UniProt

P47473 - RIR1_MYCGE

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Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene

nrdE

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei159 – 1591SubstrateBy similarity
Sitei176 – 1761Important for hydrogen atom transferBy similarity
Sitei183 – 1831Allosteric effector bindingBy similarity
Binding sitei204 – 2041Substrate; via amide nitrogenBy similarity
Sitei213 – 2131Allosteric effector bindingBy similarity
Active sitei384 – 3841Proton acceptorBy similarity
Active sitei386 – 3861Cysteine radical intermediateBy similarity
Active sitei388 – 3881Proton acceptorBy similarity
Sitei413 – 4131Important for hydrogen atom transferBy similarity
Sitei694 – 6941Important for electron transferBy similarity
Sitei695 – 6951Important for electron transferBy similarity
Sitei716 – 7161Interacts with thioredoxin/glutaredoxinBy similarity
Sitei719 – 7191Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-253-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdE
Ordered Locus Names:MG231
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Ribonucleoside-diphosphate reductase subunit alphaPRO_0000187217Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi176 ↔ 413Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

STRINGi243273.MG_231.

Structurei

3D structure databases

ProteinModelPortaliP47473.
SMRiP47473. Positions 18-700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni175 – 1762Substrate bindingBy similarity
Regioni384 – 3885Substrate bindingBy similarity
Regioni589 – 5935Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0209.
KOiK00525.
OMAiVSEASEY.
OrthoDBiEOG6J48HC.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR026459. RNR_1b_NrdE.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR013554. RNR_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PF08343. RNR_N. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
TIGR04170. RNR_1b_NrdE. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47473-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSKEKIPTF NTEEDVESYI SFNAQAKIYD DFAIDLQAVE SYIQEHVKPK
60 70 80 90 100
TKVFHSTKER LDFLIKNDYY DEKIINMYSF EQFEEITHKA YSYRFRYANF
110 120 130 140 150
MGAFKFYNAY ALKTFDGKYY LENYEDRVVM NVLMLANGNF NKALKLLKQI
160 170 180 190 200
ILNRFQPATP TFLNAGRKKR GEFVSCYLLR IEDNMESIGR AITTTLQLSK
210 220 230 240 250
RDGGVALLLS NLREAGAPIK KIENQSSGII PIMKLLEDSF SYSNQLGQRQ
260 270 280 290 300
GAGAVYLHCH HPDVMQFLDT KRENADEKIR IKSLSLGLVI PDITFQLAKN
310 320 330 340 350
NEMMALFSPY DIYQEYGKAL SDISVTEMYY ELLENQRIKK TFISARKFFQ
360 370 380 390 400
TIAELHFESG YPYILFDDTV NRRNAHKNRI VMSNLCSEIV QPSLPSEFYS
410 420 430 440 450
DLTFKKVGSD ISCNLGSLNI ARAMESGSEL AELIQLAIES LDLVSRISSL
460 470 480 490 500
ETAPSIKKGN SENHALGLGA MNLHGFLATN AIYYDSKEAV DFTNIFFYTV
510 520 530 540 550
AYHAFSASNK LALELGKFKD FENTKFADGS YFDKYTKVAS DFWTCKTEKV
560 570 580 590 600
QALFDKYQVK IPTQENWKQL VASIQKDGLA NSHLMAIAPT GSISYLSSCT
610 620 630 640 650
PSLQPVVSPV EVRKEGKLGR IYVPAYKLDN DNYQYFKDGA YELGFEPIIN
660 670 680 690 700
IVAAAQQHVD QAISLTLFMT DKATTRDLNK AYIYAFKKGC SSIYYVRVRQ
710 720
DVLKDSEDHT IKIKDCEVCS I
Length:721
Mass (Da):82,249
Last modified:February 1, 1996 - v1
Checksum:i75E0174979CCAA59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71452.1.
PIRiE64225.
RefSeqiNP_072897.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71452; AAC71452; MG_231.
GeneIDi875308.
KEGGimge:MG_231.
PATRICi20009944. VBIMycGen98045_0268.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71452.1 .
PIRi E64225.
RefSeqi NP_072897.1. NC_000908.2.

3D structure databases

ProteinModelPortali P47473.
SMRi P47473. Positions 18-700.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243273.MG_231.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC71452 ; AAC71452 ; MG_231 .
GeneIDi 875308.
KEGGi mge:MG_231.
PATRICi 20009944. VBIMycGen98045_0268.

Phylogenomic databases

eggNOGi COG0209.
KOi K00525.
OMAi VSEASEY.
OrthoDBi EOG6J48HC.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci MGEN243273:GH2R-253-MONOMER.

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR026459. RNR_1b_NrdE.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR013554. RNR_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PF08343. RNR_N. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
TIGR04170. RNR_1b_NrdE. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiRIR1_MYCGE
AccessioniPrimary (citable) accession number: P47473
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3