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P47471

- RIR2_MYCGE

UniProt

P47471 - RIR2_MYCGE

Protein

Ribonucleoside-diphosphate reductase subunit beta

Gene

nrdF

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi88 – 881Iron 1By similarity
    Metal bindingi122 – 1221Iron 1By similarity
    Active sitei126 – 1261By similarity
    Metal bindingi216 – 2161Iron 2

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
    2. deoxyribonucleotide biosynthetic process Source: InterPro
    3. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciMGEN243273:GH2R-251-MONOMER.
    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase subunit beta (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase small subunit
    Gene namesi
    Name:nrdF
    Ordered Locus Names:MG229
    OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
    Taxonomic identifieri243273 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000807: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. ribonucleoside-diphosphate reductase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 340340Ribonucleoside-diphosphate reductase subunit betaPRO_0000190483Add
    BLAST

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.By similarity

    Protein-protein interaction databases

    STRINGi243273.MG_229.

    Structurei

    3D structure databases

    ProteinModelPortaliP47471.
    SMRiP47471. Positions 18-308.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0208.
    KOiK00526.
    OMAiSAFLCYN.
    OrthoDBiEOG68DD19.

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR026494. RNR_NrdF.
    IPR000358. RNR_small.
    [Graphical view]
    PANTHERiPTHR23409. PTHR23409. 1 hit.
    PfamiPF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view]
    SUPFAMiSSF47240. SSF47240. 1 hit.
    TIGRFAMsiTIGR04171. RNR_1b_NrdF. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P47471-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAANNKKYFL ESFSPLGYVK NNFQGNLRSV NWNLVDDEKD LEVWNRIVQN    50
    FWLPEKIPVS NDIPSWKKLS KDWQDLITKT FTGLTLLDTI QATIGDICQI 100
    DHALTDHEQV IYANFAFMVG VHARSYGTIF STLCTSEQIN AAHEWVVNTE 150
    SLQKRAKALI PYYTGNDPLK SKVAAALMPG FLLYGGFYLP FYLSSRKQLP 200
    NTSDIIRLIL RDKVIHNYYS GYKYQRKLEK LPLAKQKEMK AFVFELMYRL 250
    IELEKDYLKE LYEGFGIVDD AIKFSVYNAG KFLQNLGYDS PFTAAETRIK 300
    PEIFAQLSAR ADENHDFFSG NGSSYVMGVS EETNDDDWNF 340
    Length:340
    Mass (Da):39,152
    Last modified:February 1, 1996 - v1
    Checksum:iF0ED1BDAFB2B9CFD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti234 – 24310AKQKEMKAFV → SKTKGDESIC in AAD10549. (PubMed:8253680)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71450.1.
    U01739 Genomic DNA. Translation: AAD10549.1.
    PIRiC64225.
    RefSeqiNP_072895.1. NC_000908.2.

    Genome annotation databases

    EnsemblBacteriaiAAC71450; AAC71450; MG_229.
    GeneIDi875310.
    KEGGimge:MG_229.
    PATRICi20009940. VBIMycGen98045_0266.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71450.1 .
    U01739 Genomic DNA. Translation: AAD10549.1 .
    PIRi C64225.
    RefSeqi NP_072895.1. NC_000908.2.

    3D structure databases

    ProteinModelPortali P47471.
    SMRi P47471. Positions 18-308.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243273.MG_229.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC71450 ; AAC71450 ; MG_229 .
    GeneIDi 875310.
    KEGGi mge:MG_229.
    PATRICi 20009940. VBIMycGen98045_0266.

    Phylogenomic databases

    eggNOGi COG0208.
    KOi K00526.
    OMAi SAFLCYN.
    OrthoDBi EOG68DD19.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .
    BioCyci MGEN243273:GH2R-251-MONOMER.

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR026494. RNR_NrdF.
    IPR000358. RNR_small.
    [Graphical view ]
    PANTHERi PTHR23409. PTHR23409. 1 hit.
    Pfami PF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47240. SSF47240. 1 hit.
    TIGRFAMsi TIGR04171. RNR_1b_NrdF. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33530 / G-37 / NCTC 10195.
    2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
      Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
      J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 234-340.
      Strain: ATCC 33530 / G-37 / NCTC 10195.

    Entry informationi

    Entry nameiRIR2_MYCGE
    AccessioniPrimary (citable) accession number: P47471
    Secondary accession number(s): Q49443
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Seems to lack two of the iron-binding residues.Curated

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Mycoplasma genitalium
      Mycoplasma genitalium (strain G-37): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3