P47471 (RIR2_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleoside-diphosphate reductase subunit beta
EC=1.17.4.1

Alternative name(s):
Ribonucleotide reductase small subunit

Gene names

Name:	nrdF
Ordered Locus Names:	MG229

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.
Caution	Seems to lack two of the iron-binding residues.

Ontologies

Keywords
Biological process	DNA replication
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro deoxyribonucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Ribonucleoside-diphosphate reductase subunit beta		PRO_0000190483
Sites
	Active site	126	1	By similarity
	Metal binding	88	1	Iron 1 By similarity
	Metal binding	122	1	Iron 1 By similarity
	Metal binding	216	1	Iron 2
Experimental info
	Sequence conflict	234 – 243	10	AKQKEMKAFV → SKTKGDESIC in AAD10549. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47471 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: F0ED1BDAFB2B9CFD	FASTA	340	39,152
	10 20 30 40 50 60 MAANNKKYFL ESFSPLGYVK NNFQGNLRSV NWNLVDDEKD LEVWNRIVQN FWLPEKIPVS 70 80 90 100 110 120 NDIPSWKKLS KDWQDLITKT FTGLTLLDTI QATIGDICQI DHALTDHEQV IYANFAFMVG 130 140 150 160 170 180 VHARSYGTIF STLCTSEQIN AAHEWVVNTE SLQKRAKALI PYYTGNDPLK SKVAAALMPG 190 200 210 220 230 240 FLLYGGFYLP FYLSSRKQLP NTSDIIRLIL RDKVIHNYYS GYKYQRKLEK LPLAKQKEMK 250 260 270 280 290 300 AFVFELMYRL IELEKDYLKE LYEGFGIVDD AIKFSVYNAG KFLQNLGYDS PFTAAETRIK 310 320 330 340 PEIFAQLSAR ADENHDFFSG NGSSYVMGVS EETNDDDWNF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 234-340. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71450.1. U01739 Genomic DNA. Translation: AAD10549.1.
PIR	C64225.
RefSeq	NP_072895.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47471.
SMR	P47471. Positions 18-308.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_229.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71450; AAC71450; MG_229.
GeneID	875310.
KEGG	mge:MG_229.
PATRIC	20009940. VBIMycGen98045_0266.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0208.
KO	K00526.
OMA	SGYKFQR.
ProtClustDB	PRK09614.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-251-MONOMER.
UniPathway	UPA00326.
Family and domain databases
Gene3D	1.10.620.20. 1 hit.
InterPro	IPR009078. Ferritin-like_SF. IPR012348. RNR-rel. IPR026494. RNR_1b_NrdF. IPR000358. RNR_small. [Graphical view]
PANTHER	PTHR23409. PTHR23409. 1 hit.
Pfam	PF00268. Ribonuc_red_sm. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
TIGRFAMs	TIGR04171. RNR_1b_NrdF. 1 hit.
PROSITE	PS00368. RIBORED_SMALL. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR2_MYCGE

Accession

Primary (citable) accession number: P47471
Secondary accession number(s): Q49443

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program