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P47471

- RIR2_MYCGE

UniProt

P47471 - RIR2_MYCGE

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Protein

Ribonucleoside-diphosphate reductase subunit beta

Gene

nrdF

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi88 – 881Iron 1By similarity
Metal bindingi122 – 1221Iron 1By similarity
Active sitei126 – 1261By similarity
Metal bindingi216 – 2161Iron 2

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. deoxyribonucleotide biosynthetic process Source: InterPro
  3. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-251-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit beta (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
Name:nrdF
Ordered Locus Names:MG229
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Ribonucleoside-diphosphate reductase subunit betaPRO_0000190483Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

STRINGi243273.MG_229.

Structurei

3D structure databases

ProteinModelPortaliP47471.
SMRiP47471. Positions 18-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0208.
KOiK00526.
OMAiSAFLCYN.
OrthoDBiEOG68DD19.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026494. RNR_NrdF.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR04171. RNR_1b_NrdF. 1 hit.

Sequencei

Sequence statusi: Complete.

P47471-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAANNKKYFL ESFSPLGYVK NNFQGNLRSV NWNLVDDEKD LEVWNRIVQN
60 70 80 90 100
FWLPEKIPVS NDIPSWKKLS KDWQDLITKT FTGLTLLDTI QATIGDICQI
110 120 130 140 150
DHALTDHEQV IYANFAFMVG VHARSYGTIF STLCTSEQIN AAHEWVVNTE
160 170 180 190 200
SLQKRAKALI PYYTGNDPLK SKVAAALMPG FLLYGGFYLP FYLSSRKQLP
210 220 230 240 250
NTSDIIRLIL RDKVIHNYYS GYKYQRKLEK LPLAKQKEMK AFVFELMYRL
260 270 280 290 300
IELEKDYLKE LYEGFGIVDD AIKFSVYNAG KFLQNLGYDS PFTAAETRIK
310 320 330 340
PEIFAQLSAR ADENHDFFSG NGSSYVMGVS EETNDDDWNF
Length:340
Mass (Da):39,152
Last modified:February 1, 1996 - v1
Checksum:iF0ED1BDAFB2B9CFD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti234 – 24310AKQKEMKAFV → SKTKGDESIC in AAD10549. (PubMed:8253680)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71450.1.
U01739 Genomic DNA. Translation: AAD10549.1.
PIRiC64225.
RefSeqiNP_072895.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71450; AAC71450; MG_229.
GeneIDi875310.
KEGGimge:MG_229.
PATRICi20009940. VBIMycGen98045_0266.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71450.1 .
U01739 Genomic DNA. Translation: AAD10549.1 .
PIRi C64225.
RefSeqi NP_072895.1. NC_000908.2.

3D structure databases

ProteinModelPortali P47471.
SMRi P47471. Positions 18-308.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243273.MG_229.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC71450 ; AAC71450 ; MG_229 .
GeneIDi 875310.
KEGGi mge:MG_229.
PATRICi 20009940. VBIMycGen98045_0266.

Phylogenomic databases

eggNOGi COG0208.
KOi K00526.
OMAi SAFLCYN.
OrthoDBi EOG68DD19.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci MGEN243273:GH2R-251-MONOMER.

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026494. RNR_NrdF.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
TIGRFAMsi TIGR04171. RNR_1b_NrdF. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 234-340.
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiRIR2_MYCGE
AccessioniPrimary (citable) accession number: P47471
Secondary accession number(s): Q49443
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 1, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Seems to lack two of the iron-binding residues.Curated

Keywords - Technical termi

Complete proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3