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P47470 (DYR_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase

EC=1.5.1.3
Gene names
Name:folA
Synonyms:dhfR
Ordered Locus Names:MG228
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length160 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 160160Dihydrofolate reductase
PRO_0000186395

Regions

Domain1 – 160160DHFR
Nucleotide binding6 – 72NADP By similarity
Nucleotide binding14 – 196NADP By similarity
Nucleotide binding43 – 464NADP By similarity
Nucleotide binding62 – 654NADP By similarity
Nucleotide binding101 – 1066NADP By similarity
Region5 – 73Substrate binding By similarity

Sites

Binding site271Substrate By similarity
Binding site571Substrate By similarity
Binding site1201Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P47470 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: BE46EBAE24DDBE13

FASTA16018,966
        10         20         30         40         50         60 
MLIAIWAMTQ EGLIGNNNTL PWMIKQELAH FKKTTLFQAL LMGRKTYESL PKVFEKRTIF 

        70         80         90        100        110        120 
LLSKDQNYRF EEKGSEVKVI NDFWPLIKSY QANKEKDLFI CGGKSVYEQT INECDQLIVS 

       130        140        150        160 
IIKKKYKGDQ FLKVDLSKFV LNEVVEFEEF NVNYYRKKQQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC71449.1.
PIRB64225.
RefSeqNP_072894.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47470.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_228.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC71449; AAC71449; MG_228.
GeneID875311.
KEGGmge:MG_228.
PATRIC20009938. VBIMycGen98045_0265.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0262.
KOK00287.
OMAVTFEGMK.
OrthoDBEOG6KT2V2.
ProtClustDBCLSK335209.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-250-MONOMER.
UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDYR_MYCGE
AccessionPrimary (citable) accession number: P47470
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 13, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names