P47470 (DYR_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrofolate reductase
EC=1.5.1.3

Gene names

Name:	folA
Synonyms:	dhfR
Ordered Locus Names:	MG228

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.
Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 160	160	Dihydrofolate reductase		PRO_0000186395
Regions
	Domain	1 – 160	160	DHFR
	Nucleotide binding	6 – 7	2	NADP By similarity
	Nucleotide binding	14 – 19	6	NADP By similarity
	Nucleotide binding	43 – 46	4	NADP By similarity
	Nucleotide binding	62 – 65	4	NADP By similarity
	Nucleotide binding	101 – 106	6	NADP By similarity
	Region	5 – 7	3	Substrate binding By similarity
Sites
	Binding site	27	1	Substrate By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	120	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47470 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: BE46EBAE24DDBE13	FASTA	160	18,966
	10 20 30 40 50 60 MLIAIWAMTQ EGLIGNNNTL PWMIKQELAH FKKTTLFQAL LMGRKTYESL PKVFEKRTIF 70 80 90 100 110 120 LLSKDQNYRF EEKGSEVKVI NDFWPLIKSY QANKEKDLFI CGGKSVYEQT INECDQLIVS 130 140 150 160 IIKKKYKGDQ FLKVDLSKFV LNEVVEFEEF NVNYYRKKQQ

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71449.1.
PIR	B64225.
RefSeq	NP_072894.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47470.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	875311.
GenomeReviews	Gene locus MG228 in contig L43967_GR.
KEGG	mge:MG_228.
PATRIC	20009938. VBIMycGen98045_0265.
TIGR	MG228.
Phylogenomic databases
HOGENOM	HBG665708.
OMA	YITHILQ.
PhylomeDB	P47470.
ProtClustDB	CLSK335209.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_228-MONOMER.
Family and domain databases
InterPro	IPR012259. DHFR. IPR024072. DHFR-like_dom. IPR017925. DHFR_CS. IPR001796. DHFR_dom. [Graphical view]
Gene3D	G3DSA:3.40.430.10. G3DSA:3.40.430.10. 1 hit.
KO	K00287.
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
SUPFAM	SSF53597. SSF53597. 1 hit.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR_MYCGE

Accession

Primary (citable) accession number: P47470

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program