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Protein

Thymidylate synthase

Gene

thyA

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathway: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei170 – 1701UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-249-MONOMER.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:MG227
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287Thymidylate synthasePRO_0000140983Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP47469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0207.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47469-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYLDLASY VLANGKKRKN RTDTDTLSVF GYQMKFDLTN SFPLLTTKKV
60 70 80 90 100
NWKAIVHELL WFIKGDTNIK YLVDNGVNIW NEWPYENFKK SPSFQNETLQ
110 120 130 140 150
EFILKVKTDN EFAKQFADLG PVYGKQWRNF NGVDQLKKVI QEIKENPNSR
160 170 180 190 200
RLIVSSWNPS ELEKMALAPC HSLFQFYVEE DKLSLQLYQR SGDIFLGVPF
210 220 230 240 250
NIASYALLVY LVAHETKLKP GYFIHTLGDA HIYENHIEQI KLQLTRTTLD
260 270 280
PPQVVLKSDK SIFAYSFDDI ELVGYNYHPF IYGRVAV
Length:287
Mass (Da):33,470
Last modified:February 1, 1996 - v1
Checksum:i6BA68678A9E502D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71448.1.
U01718 Unassigned DNA. Translation: AAC43192.1.
PIRiA64225.
RefSeqiNP_072893.1. NC_000908.2.
WP_010869385.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71448; AAC71448; MG_227.
KEGGimge:MG_227.
PATRICi20009936. VBIMycGen98045_0264.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71448.1.
U01718 Unassigned DNA. Translation: AAC43192.1.
PIRiA64225.
RefSeqiNP_072893.1. NC_000908.2.
WP_010869385.1. NC_000908.2.

3D structure databases

ProteinModelPortaliP47469.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71448; AAC71448; MG_227.
KEGGimge:MG_227.
PATRICi20009936. VBIMycGen98045_0264.

Phylogenomic databases

eggNOGiCOG0207.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.

Enzyme and pathway databases

UniPathwayiUPA00575.
BioCyciMGEN243273:GH2R-249-MONOMER.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 193-287.
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiTYSY_MYCGE
AccessioniPrimary (citable) accession number: P47469
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.