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P47458

- KPYK_MYCGE

UniProt

P47458 - KPYK_MYCGE

Protein

Pyruvate kinase

Gene

pyk

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Enzyme regulationi

    Regulated by phosphoenolpyruvate substrate and is allosterically activated by ribose-5-phosphate, AMP and other nucleoside monophosphates but not by fructose-1,6-bisphosphate.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei56 – 561SubstrateBy similarity
    Metal bindingi58 – 581PotassiumBy similarity
    Metal bindingi60 – 601PotassiumBy similarity
    Metal bindingi90 – 901PotassiumBy similarity
    Metal bindingi91 – 911Potassium; via carbonyl oxygenBy similarity
    Sitei249 – 2491Transition state stabilizerBy similarity
    Metal bindingi251 – 2511MagnesiumBy similarity
    Binding sitei274 – 2741Substrate; via amide nitrogenBy similarity
    Metal bindingi275 – 2751MagnesiumBy similarity
    Binding sitei275 – 2751Substrate; via amide nitrogenBy similarity
    Binding sitei307 – 3071SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    BioCyciMGEN243273:GH2R-228-MONOMER.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pyk
    Ordered Locus Names:MG216
    OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
    Taxonomic identifieri243273 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000807: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 508508Pyruvate kinasePRO_0000112079Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi243273.MG_216.

    Structurei

    3D structure databases

    ProteinModelPortaliP47458.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    KOiK00873.
    OMAiGDETMIS.
    OrthoDBiEOG6GBMB0.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 2 hits.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47458-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIDHLKRTKI IATCGPALTK SLVSLKMLDD NEYAAIKKVA YANIEAIIKS    50
    GVSVIRLNFS HGTHEEQQVR IKIVRDVAKA MNIPVSIMLD TNGPEIRIVE 100
    TKKEGLKITK DSEVIINTMS KMIASDNQFA VSDASGKYNM VNDVNIGQKI 150
    LVDDGKLTLV VTRVDKQHNQ VICVAKNDHT VFTKKRLNLP NAQYSIPFLS 200
    EKDLKDIDFG LSQGIDYIAA SFVNTVADIK QLRDYLKLKN ASGVKIIAKI 250
    ESNHALNNID KIIKASDGIM VARGDLGLEI PYYQVPYWQR YMIKACRFFN 300
    KRSITATQML DSLEKNIQPT RAEVTDVYFA VDRGNDATML SGETASGLYP 350
    LNAVAVMQKI DKQSETFFDY QYNVNYYLKN STANKSRFWH NVVLPLTKKT 400
    VPKRKLVNSA FKYDFIVYPT NNINRIYALS NARLAAAVII LTNNKRVYTG 450
    HGVDYGIFCY LIDKNPNQLT KAELIELAWK AINHYQAYGD LEKLKQCLAV 500
    YNETIINL 508
    Length:508
    Mass (Da):57,333
    Last modified:February 1, 1996 - v1
    Checksum:iE4C38BB0E92C5F8E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71435.1.
    U01798 Genomic DNA. Translation: AAD12324.1.
    PIRiH64223.
    RefSeqiNP_072881.1. NC_000908.2.

    Genome annotation databases

    EnsemblBacteriaiAAC71435; AAC71435; MG_216.
    GeneIDi875390.
    KEGGimge:MG_216.
    PATRICi20009892. VBIMycGen98045_0251.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71435.1 .
    U01798 Genomic DNA. Translation: AAD12324.1 .
    PIRi H64223.
    RefSeqi NP_072881.1. NC_000908.2.

    3D structure databases

    ProteinModelPortali P47458.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243273.MG_216.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC71435 ; AAC71435 ; MG_216 .
    GeneIDi 875390.
    KEGGi mge:MG_216.
    PATRICi 20009892. VBIMycGen98045_0251.

    Phylogenomic databases

    eggNOGi COG0469.
    KOi K00873.
    OMAi GDETMIS.
    OrthoDBi EOG6GBMB0.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    BioCyci MGEN243273:GH2R-228-MONOMER.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 2 hits.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33530 / G-37 / NCTC 10195.
    2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
      Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
      J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 264-372.
      Strain: ATCC 33530 / G-37 / NCTC 10195.

    Entry informationi

    Entry nameiKPYK_MYCGE
    AccessioniPrimary (citable) accession number: P47458
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome

    Documents

    1. Mycoplasma genitalium
      Mycoplasma genitalium (strain G-37): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3