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Protein

Pyruvate kinase

Gene

pyk

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Regulated by phosphoenolpyruvate substrate and is allosterically activated by ribose-5-phosphate, AMP and other nucleoside monophosphates but not by fructose-1,6-bisphosphate.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561SubstrateBy similarity
Metal bindingi58 – 581PotassiumBy similarity
Metal bindingi60 – 601PotassiumBy similarity
Metal bindingi90 – 901PotassiumBy similarity
Metal bindingi91 – 911Potassium; via carbonyl oxygenBy similarity
Sitei249 – 2491Transition state stabilizerBy similarity
Metal bindingi251 – 2511MagnesiumBy similarity
Binding sitei274 – 2741Substrate; via amide nitrogenBy similarity
Metal bindingi275 – 2751MagnesiumBy similarity
Binding sitei275 – 2751Substrate; via amide nitrogenBy similarity
Binding sitei307 – 3071SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-228-MONOMER.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Ordered Locus Names:MG216
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 508508Pyruvate kinasePRO_0000112079Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi243273.MG_216.

Structurei

3D structure databases

ProteinModelPortaliP47458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
KOiK00873.
OMAiPRTKLVC.
OrthoDBiEOG6GBMB0.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 2 hits.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47458-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDHLKRTKI IATCGPALTK SLVSLKMLDD NEYAAIKKVA YANIEAIIKS
60 70 80 90 100
GVSVIRLNFS HGTHEEQQVR IKIVRDVAKA MNIPVSIMLD TNGPEIRIVE
110 120 130 140 150
TKKEGLKITK DSEVIINTMS KMIASDNQFA VSDASGKYNM VNDVNIGQKI
160 170 180 190 200
LVDDGKLTLV VTRVDKQHNQ VICVAKNDHT VFTKKRLNLP NAQYSIPFLS
210 220 230 240 250
EKDLKDIDFG LSQGIDYIAA SFVNTVADIK QLRDYLKLKN ASGVKIIAKI
260 270 280 290 300
ESNHALNNID KIIKASDGIM VARGDLGLEI PYYQVPYWQR YMIKACRFFN
310 320 330 340 350
KRSITATQML DSLEKNIQPT RAEVTDVYFA VDRGNDATML SGETASGLYP
360 370 380 390 400
LNAVAVMQKI DKQSETFFDY QYNVNYYLKN STANKSRFWH NVVLPLTKKT
410 420 430 440 450
VPKRKLVNSA FKYDFIVYPT NNINRIYALS NARLAAAVII LTNNKRVYTG
460 470 480 490 500
HGVDYGIFCY LIDKNPNQLT KAELIELAWK AINHYQAYGD LEKLKQCLAV

YNETIINL
Length:508
Mass (Da):57,333
Last modified:February 1, 1996 - v1
Checksum:iE4C38BB0E92C5F8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71435.1.
U01798 Genomic DNA. Translation: AAD12324.1.
PIRiH64223.
RefSeqiNP_072881.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71435; AAC71435; MG_216.
KEGGimge:MG_216.
PATRICi20009892. VBIMycGen98045_0251.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71435.1.
U01798 Genomic DNA. Translation: AAD12324.1.
PIRiH64223.
RefSeqiNP_072881.1. NC_000908.2.

3D structure databases

ProteinModelPortaliP47458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243273.MG_216.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71435; AAC71435; MG_216.
KEGGimge:MG_216.
PATRICi20009892. VBIMycGen98045_0251.

Phylogenomic databases

eggNOGiCOG0469.
KOiK00873.
OMAiPRTKLVC.
OrthoDBiEOG6GBMB0.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.
BioCyciMGEN243273:GH2R-228-MONOMER.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 2 hits.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 264-372.
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiKPYK_MYCGE
AccessioniPrimary (citable) accession number: P47458
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 1, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.