Pyruvate kinase - Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)

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P47458 (KPYK_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 90. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Pyruvate kinase
Short name=PK
EC=2.7.1.40

Gene names

Name:	pyk
Ordered Locus Names:	MG216

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma ›

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Potassium.
Enzyme regulation	Regulated by phosphoenolpyruvate substrate and is allosterically activated by ribose-5-phosphate, AMP and other nucleoside monophosphates but not by fructose-1,6-bisphosphate By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 508

508

Pyruvate kinase

PRO_0000112079

Sites

Metal binding

Potassium By similarity

Metal binding

Potassium By similarity

Metal binding

Potassium By similarity

Metal binding

Potassium; via carbonyl oxygen By similarity

Metal binding

251

Magnesium By similarity

Metal binding

275

Magnesium By similarity

Binding site

Substrate By similarity

Binding site

274

Substrate; via amide nitrogen By similarity

Binding site

275

Substrate; via amide nitrogen By similarity

Binding site

307

Substrate By similarity

Site

249

Transition state stabilizer By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P47458 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: E4C38BB0E92C5F8E
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FASTA

508

57,333

        10         20         30         40         50         60 
MIDHLKRTKI IATCGPALTK SLVSLKMLDD NEYAAIKKVA YANIEAIIKS GVSVIRLNFS 

        70         80         90        100        110        120 
HGTHEEQQVR IKIVRDVAKA MNIPVSIMLD TNGPEIRIVE TKKEGLKITK DSEVIINTMS 

       130        140        150        160        170        180 
KMIASDNQFA VSDASGKYNM VNDVNIGQKI LVDDGKLTLV VTRVDKQHNQ VICVAKNDHT 

       190        200        210        220        230        240 
VFTKKRLNLP NAQYSIPFLS EKDLKDIDFG LSQGIDYIAA SFVNTVADIK QLRDYLKLKN 

       250        260        270        280        290        300 
ASGVKIIAKI ESNHALNNID KIIKASDGIM VARGDLGLEI PYYQVPYWQR YMIKACRFFN 

       310        320        330        340        350        360 
KRSITATQML DSLEKNIQPT RAEVTDVYFA VDRGNDATML SGETASGLYP LNAVAVMQKI 

       370        380        390        400        410        420 
DKQSETFFDY QYNVNYYLKN STANKSRFWH NVVLPLTKKT VPKRKLVNSA FKYDFIVYPT 

       430        440        450        460        470        480 
NNINRIYALS NARLAAAVII LTNNKRVYTG HGVDYGIFCY LIDKNPNQLT KAELIELAWK 

       490        500 
AINHYQAYGD LEKLKQCLAV YNETIINL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 264-372. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71435.1. U01798 Genomic DNA. Translation: AAD12324.1.
PIR	H64223.
RefSeq	NP_072881.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47458.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_216.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71435; AAC71435; MG_216.
GeneID	875390.
KEGG	mge:MG_216.
PATRIC	20009892. VBIMycGen98045_0251.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0469.
KO	K00873.
OMA	TDVYFAV.
ProtClustDB	PRK05826.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-262-MONOMER.
UniPathway	UPA00109; UER00188.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_MYCGE

Accession

Primary (citable) accession number: P47458

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents