Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P47457

- PFKA_MYCGE

UniProt

P47457 - PFKA_MYCGE

Protein

Probable ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.By similarity

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.

    Cofactori

    Magnesium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei11 – 111ATP; via amide nitrogenBy similarity
    Metal bindingi103 – 1031Magnesium; catalyticBy similarity
    Active sitei127 – 1271Proton acceptorBy similarity
    Binding sitei162 – 1621Substrate; shared with dimeric partnerBy similarity
    Binding sitei221 – 2211SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi72 – 732ATPBy similarity
    Nucleotide bindingi102 – 1054ATPBy similarity

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMGEN243273:GH2R-227-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
    Short name:
    ATP-PFK
    Short name:
    Phosphofructokinase
    Alternative name(s):
    Phosphohexokinase
    Gene namesi
    Name:pfkA
    Synonyms:pfk
    Ordered Locus Names:MG215
    OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
    Taxonomic identifieri243273 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000807: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 323323Probable ATP-dependent 6-phosphofructokinasePRO_0000111963Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer or homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi243273.MG_215.

    Structurei

    3D structure databases

    ProteinModelPortaliP47457.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1273Substrate bindingBy similarity
    Regioni169 – 1713Substrate bindingBy similarity
    Regioni253 – 2564Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0205.
    KOiK00850.
    OMAiAKLERGH.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    InterProiIPR012003. ATP_PFK_prok.
    IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P47457-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDKIAILTSG GDASGMNATI AYLTKYAIAK QLEVFYVKNG YYGLYHNHFI    50
    TSKELDLTDF FFMGGTVIGS SRFKQFQDPS LRKQAVLNLK KRGINNLVVI 100
    GGDGSYMGAK ALSELGLNCF CLPGTIDNDV NSSEFTIGFW TALEAIRVNV 150
    EAIYHTTKSH NRLAIIEVMG RDCSDLTIFG GLATNASFVV TSKNSLDLNG 200
    FEKAVRKVLQ FQNYCVVLVS ENIYGKNGLP SLEMVKEHFE NNAIKCNLVS 250
    LGHTQRGFSP NSIELFQISL MAKHTIDLVV NNANSQVIGM KNNQAVNYDF 300
    NTAFNLPKAD RTKLLNQVNT AII 323
    Length:323
    Mass (Da):35,641
    Last modified:February 1, 1996 - v1
    Checksum:i67072AC3D5542E89
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71434.1.
    PIRiG64223.
    RefSeqiNP_072880.1. NC_000908.2.

    Genome annotation databases

    EnsemblBacteriaiAAC71434; AAC71434; MG_215.
    GeneIDi875293.
    KEGGimge:MG_215.
    PATRICi20009890. VBIMycGen98045_0250.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71434.1 .
    PIRi G64223.
    RefSeqi NP_072880.1. NC_000908.2.

    3D structure databases

    ProteinModelPortali P47457.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243273.MG_215.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC71434 ; AAC71434 ; MG_215 .
    GeneIDi 875293.
    KEGGi mge:MG_215.
    PATRICi 20009890. VBIMycGen98045_0250.

    Phylogenomic databases

    eggNOGi COG0205.
    KOi K00850.
    OMAi AKLERGH.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci MGEN243273:GH2R-227-MONOMER.

    Family and domain databases

    InterProi IPR012003. ATP_PFK_prok.
    IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33530 / G-37 / NCTC 10195.

    Entry informationi

    Entry nameiPFKA_MYCGE
    AccessioniPrimary (citable) accession number: P47457
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Mycoplasma genitalium
      Mycoplasma genitalium (strain G-37): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3