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P47457 (PFKA_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Synonyms:pfk
Ordered Locus Names:MG215
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.

Cofactor

Magnesium By similarity.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Subunit structure

Homodimer or homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfructose 6-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323Probable ATP-dependent 6-phosphofructokinase
PRO_0000111963

Regions

Nucleotide binding72 – 732ATP By similarity
Nucleotide binding102 – 1054ATP By similarity
Region125 – 1273Substrate binding By similarity
Region169 – 1713Substrate binding By similarity
Region253 – 2564Substrate binding By similarity

Sites

Active site1271Proton acceptor By similarity
Metal binding1031Magnesium; catalytic By similarity
Binding site111ATP; via amide nitrogen By similarity
Binding site1621Substrate; shared with dimeric partner By similarity
Binding site2211Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P47457 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 67072AC3D5542E89

FASTA32335,641
        10         20         30         40         50         60 
MDKIAILTSG GDASGMNATI AYLTKYAIAK QLEVFYVKNG YYGLYHNHFI TSKELDLTDF 

        70         80         90        100        110        120 
FFMGGTVIGS SRFKQFQDPS LRKQAVLNLK KRGINNLVVI GGDGSYMGAK ALSELGLNCF 

       130        140        150        160        170        180 
CLPGTIDNDV NSSEFTIGFW TALEAIRVNV EAIYHTTKSH NRLAIIEVMG RDCSDLTIFG 

       190        200        210        220        230        240 
GLATNASFVV TSKNSLDLNG FEKAVRKVLQ FQNYCVVLVS ENIYGKNGLP SLEMVKEHFE 

       250        260        270        280        290        300 
NNAIKCNLVS LGHTQRGFSP NSIELFQISL MAKHTIDLVV NNANSQVIGM KNNQAVNYDF 

       310        320 
NTAFNLPKAD RTKLLNQVNT AII 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC71434.1.
PIRG64223.
RefSeqNP_072880.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47457.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_215.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC71434; AAC71434; MG_215.
GeneID875293.
KEGGmge:MG_215.
PATRIC20009890. VBIMycGen98045_0250.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0205.
KOK00850.
OMAAKLERGH.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-227-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

InterProIPR012003. ATP_PFK_prok.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
ProtoNetSearch...

Entry information

Entry namePFKA_MYCGE
AccessionPrimary (citable) accession number: P47457
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names