DNA topoisomerase 4 subunit A - Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)

Contribute

Read comments (?) or add your own

P47446 (PARC_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

to top of page

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

to top of page

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA topoisomerase 4 subunit A
EC=5.99.1.3

Alternative name(s):
Topoisomerase IV subunit A

Gene names

Name:	parC
Ordered Locus Names:	MG204

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma ›

Protein attributes

Sequence length	781 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule By similarity. HAMAP-Rule MF_00937
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_00937
Subunit structure	Heterotetramer composed of ParC and ParE By similarity. HAMAP-Rule MF_00937
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_00937.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. ParC type 2 subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	DNA-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

781

DNA topoisomerase 4 subunit A HAMAP-Rule MF_00937

PRO_0000145401

Sites

Active site

1

O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Site

1

Interaction with DNA By similarity

Site

1

Interaction with DNA By similarity

Site

1

Interaction with DNA By similarity

Site

1

Interaction with DNA By similarity

Site

1

Interaction with DNA By similarity

Site

1

Transition state stabilizer By similarity

Experimental info

Sequence conflict

1

P → R in AAC43991. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P47446 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: F14319CEE305B437
Show »

781

88,513

        10         20         30         40         50         60 
MDQKNNNLFQ KAIEEVFAVS FSKYAKYIIQ DRALPDLRDG LKPVQRRILY GMFQMGLKPT 

        70         80         90        100        110        120 
TPYKKSARAV GEIMGKYHPH GDSSIYDAII RMSQSWKNNW TTVSIHGNNG SVDGDNAAAM 

       130        140        150        160        170        180 
RYTETRLSLY GFELLKDIDK KLVSFINNFD DSEKEPTVLP TLLPNLFING ASGIAAGYAT 

       190        200        210        220        230        240 
NIAPHNTNEL LDSLCLRIDQ PNCELKQILK IVKGPDFPTG GNVYFEKSLS DIYQAGKGKF 

       250        260        270        280        290        300 
IIQAKYEVNK NLNQIEITQI PYETLKANIV KQIEEIIFDN KLSAIESVID SSDRNGIRII 

       310        320        330        340        350        360 
IKHKDFLPAE KIMAFLFKHT QLQVNFNLNN TVIANRFPIQ IGLLSYLDHF LKFCHELIIN 

       370        380        390        400        410        420 
KAKYELELAS KRLEIILGLI KAISIIDKII KLIRSAVDKS DAREKLIDNF KFTFNQAEAI 

       430        440        450        460        470        480 
VSLRLYQLTN TDIFELNQEQ NELEKTVISS EQLIASEKAR NKLLKKQFEG YKKQFHQQRR 

       490        500        510        520        530        540 
SQICGFINQK KVEESELIEN KTYGVLITKA GNYHKFESNQ LLKSTTDFKS ESDTIIFAQT 

       550        560        570        580        590        600 
IANTDQIFIV TSLGNIINIP VYKLAFNSKN KLASLVSKKP ILLEYETIVF VGTMNSVNQP 

       610        620        630        640        650        660 
ILVLTSKLGM VKRIDLTKLN IKPLKATLCI SLRDKDHLVS AFLQQDDKLI CLVSDHNYYT 

       670        680        690        700        710        720 
VFHTNEIPLI SSKGMGVKGM KLKLEDQIKF VVAFEANEPL VMICSDGSVI NLKQTELVVV 

       730        740        750        760        770        780 
SRMATAKKLP VKKAINYCFS DATNTQLINF QGKNGSKLIT TSELNQMSKT AISQTRFNKL 


N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	Bailey C.C., Younkins R., Huang W.M., Bott K.F. Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-479. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71422.1. U25549 Genomic DNA. Translation: AAC43991.1.
PIR	E64222.
RefSeq	NP_072866.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47446.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_204.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71422; AAC71422; MG_204.
GeneID	875340.
KEGG	mge:MG_204.
PATRIC	20009862. VBIMycGen98045_0236.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0188.
KO	K02621.
OMA	FATEMPP.
ProtClustDB	CLSK335060.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-213-MONOMER.
Family and domain databases
Gene3D	3.30.1360.40. 1 hit. 3.90.199.10. 2 hits.
HAMAP	MF_00937. ParC_type2.
InterPro	IPR024946. Arg_repress_C-like. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_like_dom. IPR005741. TopoIV_A_Gpos. [Graphical view]
Pfam	PF03989. DNA_gyraseA_C. 3 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01061. parC_Gpos. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PARC_MYCGE

Accession

Primary (citable) accession number: P47446
Secondary accession number(s): Q49377

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of page

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents