P47443 (GRPE_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein GrpE

Alternative name(s):
HSP-70 cofactor

Gene names

Name:	grpE
Ordered Locus Names:	MG201

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding By similarity. HAMAP-Rule MF_01151
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GrpE family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	adenyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	Protein GrpE HAMAP-Rule MF_01151		PRO_0000113816

Sequences

	Sequence		Length	Mass (Da)
	P47443 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 9643920BB7084003	FASTA	217	25,014
	10 20 30 40 50 60 MCEKSQTIKE LLNAIRTLVV KNNKAKVSMI EKELLAFVSE LDKKFKQQLN NFNELQQKIP 70 80 90 100 110 120 LLQKANEEFA LKFERMQREA QNQIQAKLDE LNLKNKKELE QAKKYAIAKT LDQPLNIIDQ 130 140 150 160 170 180 FEIALSYAQK DPQVKNYTTG FTMVLDAFSR WLEANGVTKI KIEPGMEFDE KIMSALELVD 190 200 210 SNLAKNKVVR VSKSGYKLYD KVIRFASVFV SKGNKKS

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71419.1.
PIR	B64222.
RefSeq	NP_072863.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47443.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_201.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71419; AAC71419; MG_201.
GeneID	875689.
KEGG	mge:MG_201.
PATRIC	20009854. VBIMycGen98045_0233.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0576.
KO	K03687.
OMA	IDQFEIA.
ProtClustDB	CLSK378131.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-244-MONOMER.
Family and domain databases
Gene3D	2.30.22.10. 1 hit. 3.90.20.20. 1 hit.
HAMAP	MF_01151. GrpE.
InterPro	IPR000740. GrpE. IPR013805. GrpE_coiled_coil. IPR009012. GrpE_head. [Graphical view]
PANTHER	PTHR21237. PTHR21237. 1 hit.
Pfam	PF01025. GrpE. 1 hit. [Graphical view]
PRINTS	PR00773. GRPEPROTEIN.
SUPFAM	SSF51064. GrpE_head. 1 hit.
PROSITE	PS01071. GRPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRPE_MYCGE

Accession

Primary (citable) accession number: P47443

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program