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P47436

- SYFA_MYCGE

UniProt

P47436 - SYFA_MYCGE

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Protein

Phenylalanine--tRNA ligase alpha subunit

Gene

pheS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per tetramer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi254 – 2541MagnesiumBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-202-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase alpha subunit (EC:6.1.1.20)
Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit
Short name:
PheRS
Gene namesi
Name:pheS
Ordered Locus Names:MG194
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341Phenylalanine--tRNA ligase alpha subunitPRO_0000126729Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

STRINGi243273.MG_194.

Structurei

3D structure databases

ProteinModelPortaliP47436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0016.
KOiK01889.
OMAiVEAEWFN.
OrthoDBiEOG6WX4QN.

Family and domain databases

HAMAPiMF_00281. Phe_tRNA_synth_alpha1.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR022911. Phe_tRNA_ligase_alpha1_bac.
IPR002319. Phenylalanyl-tRNA_Synthase.
[Graphical view]
PfamiPF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47436-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIDQNKLITK WKKAFAKAKN LTTLVNLKNT LHNSDLKPLL QKIKTATKLS
60 70 80 90 100
EKSSLGKLYQ SLDIQLTDLL TSYKKTFEIN NQVSQKPSLD VMLPATEFTN
110 120 130 140 150
GSNNALYQVI DNLVEYFKSF LFTINFDSEL TSISDCFDLL NIPKDHSSRN
160 170 180 190 200
ESDSFYIDKT SLLRTHCTAT TLKAVRTSKK TNNPDIRVVS LGAVFRNDSD
210 220 230 240 250
DATHSHQFTQ LDFMWIKKGL SLANLKWFIN NMITHFFGEN TFTRFRLSHF
260 270 280 290 300
PFTEPSFEID IRCWLCQNGC SICKQTKWIE ILGAGIIHPQ VMNNMGIGDT
310 320 330 340
ENITGIAAGI GIERLAMLKY GIDDIRDFYD NNFKFLTQFT D
Length:341
Mass (Da):38,982
Last modified:February 1, 1996 - v1
Checksum:i1C6FAA7663C7A01D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti232 – 2387MITHFFG → YDHPFLW(PubMed:8253680)Curated
Sequence conflicti324 – 33411DIRDFYDNNFK → VYLIFMITTLS(PubMed:8253680)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71412.1.
U02120 Genomic DNA. Translation: AAD12394.1.
U01711 Genomic DNA. Translation: AAB01023.1.
PIRiD64221.
RefSeqiNP_072856.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71412; AAC71412; MG_194.
GeneIDi875437.
KEGGimge:MG_194.
PATRICi20009840. VBIMycGen98045_0226.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71412.1 .
U02120 Genomic DNA. Translation: AAD12394.1 .
U01711 Genomic DNA. Translation: AAB01023.1 .
PIRi D64221.
RefSeqi NP_072856.1. NC_000908.2.

3D structure databases

ProteinModelPortali P47436.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243273.MG_194.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC71412 ; AAC71412 ; MG_194 .
GeneIDi 875437.
KEGGi mge:MG_194.
PATRICi 20009840. VBIMycGen98045_0226.

Phylogenomic databases

eggNOGi COG0016.
KOi K01889.
OMAi VEAEWFN.
OrthoDBi EOG6WX4QN.

Enzyme and pathway databases

BioCyci MGEN243273:GH2R-202-MONOMER.

Family and domain databases

HAMAPi MF_00281. Phe_tRNA_synth_alpha1.
InterProi IPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR022911. Phe_tRNA_ligase_alpha1_bac.
IPR002319. Phenylalanyl-tRNA_Synthase.
[Graphical view ]
Pfami PF01409. tRNA-synt_2d. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00468. pheS. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 66-119 AND 232-335.
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiSYFA_MYCGE
AccessioniPrimary (citable) accession number: P47436
Secondary accession number(s): Q49515
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 26, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3