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Protein

Phenylalanine--tRNA ligase alpha subunit

Gene

pheS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per tetramer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi254 – 2541MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-202-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase alpha subunit (EC:6.1.1.20)
Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit
Short name:
PheRS
Gene namesi
Name:pheS
Ordered Locus Names:MG194
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341Phenylalanine--tRNA ligase alpha subunitPRO_0000126729Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Structurei

3D structure databases

ProteinModelPortaliP47436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0016.
KOiK01889.
OrthoDBiEOG6WX4QN.

Family and domain databases

HAMAPiMF_00281. Phe_tRNA_synth_alpha1.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR022911. Phe_tRNA_ligase_alpha1_bac.
IPR002319. Phenylalanyl-tRNA_Synthase.
[Graphical view]
PfamiPF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47436-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDQNKLITK WKKAFAKAKN LTTLVNLKNT LHNSDLKPLL QKIKTATKLS
60 70 80 90 100
EKSSLGKLYQ SLDIQLTDLL TSYKKTFEIN NQVSQKPSLD VMLPATEFTN
110 120 130 140 150
GSNNALYQVI DNLVEYFKSF LFTINFDSEL TSISDCFDLL NIPKDHSSRN
160 170 180 190 200
ESDSFYIDKT SLLRTHCTAT TLKAVRTSKK TNNPDIRVVS LGAVFRNDSD
210 220 230 240 250
DATHSHQFTQ LDFMWIKKGL SLANLKWFIN NMITHFFGEN TFTRFRLSHF
260 270 280 290 300
PFTEPSFEID IRCWLCQNGC SICKQTKWIE ILGAGIIHPQ VMNNMGIGDT
310 320 330 340
ENITGIAAGI GIERLAMLKY GIDDIRDFYD NNFKFLTQFT D
Length:341
Mass (Da):38,982
Last modified:February 1, 1996 - v1
Checksum:i1C6FAA7663C7A01D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti232 – 2387MITHFFG → YDHPFLW (PubMed:8253680).Curated
Sequence conflicti324 – 33411DIRDFYDNNFK → VYLIFMITTLS (PubMed:8253680).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71412.1.
U02120 Genomic DNA. Translation: AAD12394.1.
U01711 Genomic DNA. Translation: AAB01023.1.
PIRiD64221.
RefSeqiNP_072856.1. NC_000908.2.
WP_010869368.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71412; AAC71412; MG_194.
KEGGimge:MG_194.
PATRICi20009840. VBIMycGen98045_0226.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71412.1.
U02120 Genomic DNA. Translation: AAD12394.1.
U01711 Genomic DNA. Translation: AAB01023.1.
PIRiD64221.
RefSeqiNP_072856.1. NC_000908.2.
WP_010869368.1. NC_000908.2.

3D structure databases

ProteinModelPortaliP47436.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71412; AAC71412; MG_194.
KEGGimge:MG_194.
PATRICi20009840. VBIMycGen98045_0226.

Phylogenomic databases

eggNOGiCOG0016.
KOiK01889.
OrthoDBiEOG6WX4QN.

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-202-MONOMER.

Family and domain databases

HAMAPiMF_00281. Phe_tRNA_synth_alpha1.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR022911. Phe_tRNA_ligase_alpha1_bac.
IPR002319. Phenylalanyl-tRNA_Synthase.
[Graphical view]
PfamiPF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 66-119 AND 232-335.
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiSYFA_MYCGE
AccessioniPrimary (citable) accession number: P47436
Secondary accession number(s): Q49515
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.