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P47436 (SYFA_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phenylalanine--tRNA ligase alpha subunit

EC=6.1.1.20
Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit
Short name=PheRS
Gene names
Name:pheS
Ordered Locus Names:MG194
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00281

Cofactor

Binds 2 magnesium ions per tetramer By similarity. HAMAP-Rule MF_00281

Subunit structure

Tetramer of two alpha and two beta subunits By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00281.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processphenylalanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phenylalanine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341Phenylalanine--tRNA ligase alpha subunit HAMAP-Rule MF_00281
PRO_0000126729

Sites

Metal binding2541Magnesium By similarity

Experimental info

Sequence conflict232 – 2387MITHFFG → YDHPFLW Ref.2
Sequence conflict324 – 33411DIRDFYDNNFK → VYLIFMITTLS Ref.2

Sequences

Sequence LengthMass (Da)Tools
P47436 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 1C6FAA7663C7A01D

FASTA34138,982
        10         20         30         40         50         60 
MIDQNKLITK WKKAFAKAKN LTTLVNLKNT LHNSDLKPLL QKIKTATKLS EKSSLGKLYQ 

        70         80         90        100        110        120 
SLDIQLTDLL TSYKKTFEIN NQVSQKPSLD VMLPATEFTN GSNNALYQVI DNLVEYFKSF 

       130        140        150        160        170        180 
LFTINFDSEL TSISDCFDLL NIPKDHSSRN ESDSFYIDKT SLLRTHCTAT TLKAVRTSKK 

       190        200        210        220        230        240 
TNNPDIRVVS LGAVFRNDSD DATHSHQFTQ LDFMWIKKGL SLANLKWFIN NMITHFFGEN 

       250        260        270        280        290        300 
TFTRFRLSHF PFTEPSFEID IRCWLCQNGC SICKQTKWIE ILGAGIIHPQ VMNNMGIGDT 

       310        320        330        340 
ENITGIAAGI GIERLAMLKY GIDDIRDFYD NNFKFLTQFT D 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC71412.1.
U02120 Genomic DNA. Translation: AAD12394.1.
U01711 Genomic DNA. Translation: AAB01023.1.
PIRD64221.
RefSeqNP_072856.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47436.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_194.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC71412; AAC71412; MG_194.
GeneID875437.
KEGGmge:MG_194.
PATRIC20009840. VBIMycGen98045_0226.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0016.
KOK01889.
OMAVEAEWFN.
OrthoDBEOG6WX4QN.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-202-MONOMER.

Family and domain databases

HAMAPMF_00281. Phe_tRNA_synth_alpha1.
InterProIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR022911. Phe_tRNA_ligase_alpha1_bac.
IPR002319. Phenylalanyl-tRNA_Synthase.
[Graphical view]
PfamPF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
TIGRFAMsTIGR00468. pheS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYFA_MYCGE
AccessionPrimary (citable) accession number: P47436
Secondary accession number(s): Q49515
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries