P47436 (SYFA_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha subunit
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	MG194

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Phenylalanine--tRNA ligase alpha subunit HAMAP-Rule MF_00281		PRO_0000126729
Sites
	Metal binding	254	1	Magnesium By similarity
Experimental info
	Sequence conflict	232 – 238	7	MITHFFG → YDHPFLW Ref.2
	Sequence conflict	324 – 334	11	DIRDFYDNNFK → VYLIFMITTLS Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47436 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 1C6FAA7663C7A01D	FASTA	341	38,982
	10 20 30 40 50 60 MIDQNKLITK WKKAFAKAKN LTTLVNLKNT LHNSDLKPLL QKIKTATKLS EKSSLGKLYQ 70 80 90 100 110 120 SLDIQLTDLL TSYKKTFEIN NQVSQKPSLD VMLPATEFTN GSNNALYQVI DNLVEYFKSF 130 140 150 160 170 180 LFTINFDSEL TSISDCFDLL NIPKDHSSRN ESDSFYIDKT SLLRTHCTAT TLKAVRTSKK 190 200 210 220 230 240 TNNPDIRVVS LGAVFRNDSD DATHSHQFTQ LDFMWIKKGL SLANLKWFIN NMITHFFGEN 250 260 270 280 290 300 TFTRFRLSHF PFTEPSFEID IRCWLCQNGC SICKQTKWIE ILGAGIIHPQ VMNNMGIGDT 310 320 330 340 ENITGIAAGI GIERLAMLKY GIDDIRDFYD NNFKFLTQFT D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 66-119 AND 232-335. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71412.1. U02120 Genomic DNA. Translation: AAD12394.1. U01711 Genomic DNA. Translation: AAB01023.1.
PIR	D64221.
RefSeq	NP_072856.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47436.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_194.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71412; AAC71412; MG_194.
GeneID	875437.
KEGG	mge:MG_194.
PATRIC	20009840. VBIMycGen98045_0226.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
KO	K01889.
OMA	FEIDIRC.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-237-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1.
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR022911. Phe_tRNA_ligase_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. [Graphical view]
Pfam	PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_MYCGE

Accession

Primary (citable) accession number: P47436
Secondary accession number(s): Q49515

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program