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P47436

- SYFA_MYCGE

UniProt

P47436 - SYFA_MYCGE

Protein

Phenylalanine--tRNA ligase alpha subunit

Gene

pheS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

    Cofactori

    Binds 2 magnesium ions per tetramer.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi254 – 2541MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMGEN243273:GH2R-202-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phenylalanine--tRNA ligase alpha subunit (EC:6.1.1.20)
    Alternative name(s):
    Phenylalanyl-tRNA synthetase alpha subunit
    Short name:
    PheRS
    Gene namesi
    Name:pheS
    Ordered Locus Names:MG194
    OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
    Taxonomic identifieri243273 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000807: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 341341Phenylalanine--tRNA ligase alpha subunitPRO_0000126729Add
    BLAST

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.By similarity

    Protein-protein interaction databases

    STRINGi243273.MG_194.

    Structurei

    3D structure databases

    ProteinModelPortaliP47436.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0016.
    KOiK01889.
    OMAiVEAEWFN.
    OrthoDBiEOG6WX4QN.

    Family and domain databases

    HAMAPiMF_00281. Phe_tRNA_synth_alpha1.
    InterProiIPR006195. aa-tRNA-synth_II.
    IPR004529. Phe-tRNA-synth_IIc_asu.
    IPR022911. Phe_tRNA_ligase_alpha1_bac.
    IPR002319. Phenylalanyl-tRNA_Synthase.
    [Graphical view]
    PfamiPF01409. tRNA-synt_2d. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00468. pheS. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47436-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIDQNKLITK WKKAFAKAKN LTTLVNLKNT LHNSDLKPLL QKIKTATKLS    50
    EKSSLGKLYQ SLDIQLTDLL TSYKKTFEIN NQVSQKPSLD VMLPATEFTN 100
    GSNNALYQVI DNLVEYFKSF LFTINFDSEL TSISDCFDLL NIPKDHSSRN 150
    ESDSFYIDKT SLLRTHCTAT TLKAVRTSKK TNNPDIRVVS LGAVFRNDSD 200
    DATHSHQFTQ LDFMWIKKGL SLANLKWFIN NMITHFFGEN TFTRFRLSHF 250
    PFTEPSFEID IRCWLCQNGC SICKQTKWIE ILGAGIIHPQ VMNNMGIGDT 300
    ENITGIAAGI GIERLAMLKY GIDDIRDFYD NNFKFLTQFT D 341
    Length:341
    Mass (Da):38,982
    Last modified:February 1, 1996 - v1
    Checksum:i1C6FAA7663C7A01D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti232 – 2387MITHFFG → YDHPFLW(PubMed:8253680)Curated
    Sequence conflicti324 – 33411DIRDFYDNNFK → VYLIFMITTLS(PubMed:8253680)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71412.1.
    U02120 Genomic DNA. Translation: AAD12394.1.
    U01711 Genomic DNA. Translation: AAB01023.1.
    PIRiD64221.
    RefSeqiNP_072856.1. NC_000908.2.

    Genome annotation databases

    EnsemblBacteriaiAAC71412; AAC71412; MG_194.
    GeneIDi875437.
    KEGGimge:MG_194.
    PATRICi20009840. VBIMycGen98045_0226.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71412.1 .
    U02120 Genomic DNA. Translation: AAD12394.1 .
    U01711 Genomic DNA. Translation: AAB01023.1 .
    PIRi D64221.
    RefSeqi NP_072856.1. NC_000908.2.

    3D structure databases

    ProteinModelPortali P47436.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243273.MG_194.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC71412 ; AAC71412 ; MG_194 .
    GeneIDi 875437.
    KEGGi mge:MG_194.
    PATRICi 20009840. VBIMycGen98045_0226.

    Phylogenomic databases

    eggNOGi COG0016.
    KOi K01889.
    OMAi VEAEWFN.
    OrthoDBi EOG6WX4QN.

    Enzyme and pathway databases

    BioCyci MGEN243273:GH2R-202-MONOMER.

    Family and domain databases

    HAMAPi MF_00281. Phe_tRNA_synth_alpha1.
    InterProi IPR006195. aa-tRNA-synth_II.
    IPR004529. Phe-tRNA-synth_IIc_asu.
    IPR022911. Phe_tRNA_ligase_alpha1_bac.
    IPR002319. Phenylalanyl-tRNA_Synthase.
    [Graphical view ]
    Pfami PF01409. tRNA-synt_2d. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00468. pheS. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33530 / G-37 / NCTC 10195.
    2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
      Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
      J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 66-119 AND 232-335.
      Strain: ATCC 33530 / G-37 / NCTC 10195.

    Entry informationi

    Entry nameiSYFA_MYCGE
    AccessioniPrimary (citable) accession number: P47436
    Secondary accession number(s): Q49515
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Mycoplasma genitalium
      Mycoplasma genitalium (strain G-37): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3