P47391 (RIBF_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative riboflavin biosynthesis protein RibF

Including the following 2 domains:

1. Riboflavin kinase
   EC=2.7.1.26
   Alternative name(s):
   Flavokinase
2. FMN adenylyltransferase
   EC=2.7.7.2
   Alternative name(s):
   FAD pyrophosphorylase
   FAD synthase

Gene names

Name:	ribF
Ordered Locus Names:	MG145

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + riboflavin = ADP + FMN. ATP + FMN = diphosphate + FAD.
Pathway	Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.
Sequence similarities	Belongs to the RibF family.

Ontologies

Keywords
Ligand	ATP-binding FAD FMN Nucleotide-binding
Molecular function	Kinase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	FAD biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway FMN biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW FMN adenylyltransferase activity Inferred from electronic annotation. Source: EC riboflavin kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Putative riboflavin biosynthesis protein RibF		PRO_0000194145

Sequences

	Sequence		Length	Mass (Da)
	P47391 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: A6F669F0450D03B0	FASTA	269	31,091
	10 20 30 40 50 60 MKSLFIGYFD GLHQGHLFLK QNSKFEPMVL LIDNPPLKQT NWLYDLQQRV AQIKTYLKAT 70 80 90 100 110 120 VEVFDVAKHN MNALSFFEQQ IKRLNCDEII VGTDWHFGND HKDGIWLKKL FKNTVIVNKT 130 140 150 160 170 180 NLSSSVIRNY LTNNELEKAN QLLVEPYYRV GTVVHGLKKA RLLGFPTANI VMDNHLLTLN 190 200 210 220 230 240 KGSYIVRVLL NNQTFYGIGF ISQKDQDLVC ETHIFNFNNE IYGSLVKFTL LKFIRTISKF 250 260 SSQAALQKAI QSDANFALKW LENQNLDKI

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71363.1.
PIR	A64216.
RefSeq	NP_072807.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47391.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_145.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71363; AAC71363; MG_145.
GeneID	875524.
KEGG	mge:MG_145.
PATRIC	20009720. VBIMycGen98045_0166.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0196.
KO	K11753.
OMA	CETHIFN.
ProtClustDB	CLSK335159.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-188-MONOMER.
UniPathway	UPA00276; UER00406. UPA00277; UER00407.
Family and domain databases
Gene3D	2.40.30.30. 1 hit. 3.40.50.620. 1 hit.
InterPro	IPR004821. Cyt_trans-like. IPR015864. FAD_synthase. IPR023468. Riboflavin_kinase. IPR002606. Riboflavin_kinase_bac. IPR015865. Riboflavin_kinase_bac/euk. IPR023465. Riboflavin_kinase_domain. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR22749. PTHR22749. 1 hit.
Pfam	PF06574. FAD_syn. 1 hit. PF01687. Flavokinase. 1 hit. [Graphical view]
PIRSF	PIRSF004491. FAD_Synth. 1 hit.
SMART	SM00904. Flavokinase. 1 hit. [Graphical view]
SUPFAM	SSF82114. Riboflavin_kinase. 1 hit.
TIGRFAMs	TIGR00125. cyt_tran_rel. 1 hit. TIGR00083. ribF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBF_MYCGE

Accession

Primary (citable) accession number: P47391

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program