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P47382 (SYK_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysine--tRNA ligase

EC=6.1.1.6
Alternative name(s):
Lysyl-tRNA synthetase
Short name=LysRS
Gene names
Name:lysS
Ordered Locus Names:MG136
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP-Rule MF_00252

Cofactor

Binds 3 magnesium ions per subunit By similarity. HAMAP-Rule MF_00252

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00252

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00252.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlysyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

lysine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 490490Lysine--tRNA ligase HAMAP-Rule MF_00252
PRO_0000152646

Sites

Metal binding4001Magnesium 1 By similarity
Metal binding4071Magnesium 1 By similarity
Metal binding4071Magnesium 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P47382 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 7F0BB0D76A0C13C6

FASTA49056,565
        10         20         30         40         50         60 
MSDRLNDQAQ HRLQKLLRLK QTNNDPYLVT KTSLTHSSKS FQVEFEKCSE EELKKKATVS 

        70         80         90        100        110        120 
LAGRIIAIRQ TFLIIQDFDG QVQLYINKKI HPKLFDYFNE FVDIGDQIVV SGKPMLTKTK 

       130        140        150        160        170        180 
VLTLAVEEMK IIAKCLLVPP EKWHGLTDIE TRARKRFLDL TYNLAMRDVF LKRTKIIKSI 

       190        200        210        220        230        240 
RSFLDQNGFI EVETPTLQAV LGGANAKPFK THYNALKADF YLRIANEIAL KKLIIGGFNK 

       250        260        270        280        290        300 
VYEMGKMFRN EGVDTTHNPE FTSIEIYQAY ADFEVMLVLV EKLIQSLCES LNQFSFNWNN 

       310        320        330        340        350        360 
KTINLKTPFH KITMVELIKK VTGIDFNSVK DDQSAILLAE KHHVKLAKHQ QNKQHIINLF 

       370        380        390        400        410        420 
FEQFCEQTLI EPTFVTHYPK AVSPLAKQDP SNPEFTQRFE LFINGKEIAN AYSELNDPLE 

       430        440        450        460        470        480 
QRKRFEQQLE EKQLGNDETS ELDESFLEAL SFGMVNTAGL GIGIDRLVML LCECNSIRDV 

       490 
VFFPQLREHK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC71353.1.
PIRA64215.
RefSeqNP_072798.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47382.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_136.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC71353; AAC71353; MG_136.
GeneID875448.
KEGGmge:MG_136.
PATRIC20009678. VBIMycGen98045_0148.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1190.
KOK04567.
OMAEINIHEA.
OrthoDBEOG69PQ2M.
ProtClustDBPRK00484.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-140-MONOMER.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
HAMAPMF_00252. Lys_tRNA_synth_class2.
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF4. PTHR22594:SF4. 1 hit.
PfamPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSPR00982. TRNASYNTHLYS.
SUPFAMSSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00499. lysS_bact. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYK_MYCGE
AccessionPrimary (citable) accession number: P47382
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 19, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries