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P47382

- SYK_MYCGE

UniProt

P47382 - SYK_MYCGE

Protein

Lysine--tRNA ligase

Gene

lysS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).

    Cofactori

    Binds 3 magnesium ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi400 – 4001Magnesium 1By similarity
    Metal bindingi407 – 4071Magnesium 1By similarity
    Metal bindingi407 – 4071Magnesium 2By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. lysine-tRNA ligase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. lysyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMGEN243273:GH2R-140-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysine--tRNA ligase (EC:6.1.1.6)
    Alternative name(s):
    Lysyl-tRNA synthetase
    Short name:
    LysRS
    Gene namesi
    Name:lysS
    Ordered Locus Names:MG136
    OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
    Taxonomic identifieri243273 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000807: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 490490Lysine--tRNA ligasePRO_0000152646Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi243273.MG_136.

    Structurei

    3D structure databases

    ProteinModelPortaliP47382.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1190.
    KOiK04567.
    OMAiMAETENK.
    OrthoDBiEOG69PQ2M.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    HAMAPiMF_00252. Lys_tRNA_synth_class2.
    InterProiIPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR002313. Lys-tRNA-ligase_II.
    IPR018149. Lys-tRNA-synth_II_C.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view]
    PANTHERiPTHR22594. PTHR22594. 1 hit.
    PfamiPF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view]
    PRINTSiPR00982. TRNASYNTHLYS.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47382-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDRLNDQAQ HRLQKLLRLK QTNNDPYLVT KTSLTHSSKS FQVEFEKCSE    50
    EELKKKATVS LAGRIIAIRQ TFLIIQDFDG QVQLYINKKI HPKLFDYFNE 100
    FVDIGDQIVV SGKPMLTKTK VLTLAVEEMK IIAKCLLVPP EKWHGLTDIE 150
    TRARKRFLDL TYNLAMRDVF LKRTKIIKSI RSFLDQNGFI EVETPTLQAV 200
    LGGANAKPFK THYNALKADF YLRIANEIAL KKLIIGGFNK VYEMGKMFRN 250
    EGVDTTHNPE FTSIEIYQAY ADFEVMLVLV EKLIQSLCES LNQFSFNWNN 300
    KTINLKTPFH KITMVELIKK VTGIDFNSVK DDQSAILLAE KHHVKLAKHQ 350
    QNKQHIINLF FEQFCEQTLI EPTFVTHYPK AVSPLAKQDP SNPEFTQRFE 400
    LFINGKEIAN AYSELNDPLE QRKRFEQQLE EKQLGNDETS ELDESFLEAL 450
    SFGMVNTAGL GIGIDRLVML LCECNSIRDV VFFPQLREHK 490
    Length:490
    Mass (Da):56,565
    Last modified:February 1, 1996 - v1
    Checksum:i7F0BB0D76A0C13C6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71353.1.
    PIRiA64215.
    RefSeqiNP_072798.1. NC_000908.2.

    Genome annotation databases

    EnsemblBacteriaiAAC71353; AAC71353; MG_136.
    GeneIDi875448.
    KEGGimge:MG_136.
    PATRICi20009678. VBIMycGen98045_0148.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71353.1 .
    PIRi A64215.
    RefSeqi NP_072798.1. NC_000908.2.

    3D structure databases

    ProteinModelPortali P47382.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243273.MG_136.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC71353 ; AAC71353 ; MG_136 .
    GeneIDi 875448.
    KEGGi mge:MG_136.
    PATRICi 20009678. VBIMycGen98045_0148.

    Phylogenomic databases

    eggNOGi COG1190.
    KOi K04567.
    OMAi MAETENK.
    OrthoDBi EOG69PQ2M.

    Enzyme and pathway databases

    BioCyci MGEN243273:GH2R-140-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    HAMAPi MF_00252. Lys_tRNA_synth_class2.
    InterProi IPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR002313. Lys-tRNA-ligase_II.
    IPR018149. Lys-tRNA-synth_II_C.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view ]
    PANTHERi PTHR22594. PTHR22594. 1 hit.
    Pfami PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view ]
    PRINTSi PR00982. TRNASYNTHLYS.
    SUPFAMi SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00499. lysS_bact. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33530 / G-37 / NCTC 10195.

    Entry informationi

    Entry nameiSYK_MYCGE
    AccessioniPrimary (citable) accession number: P47382
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Mycoplasma genitalium
      Mycoplasma genitalium (strain G-37): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3