Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lysine--tRNA ligase

Gene

lysS

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi400 – 4001Magnesium 1By similarity
Metal bindingi407 – 4071Magnesium 1By similarity
Metal bindingi407 – 4071Magnesium 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-140-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligase (EC:6.1.1.6)
Alternative name(s):
Lysyl-tRNA synthetase
Short name:
LysRS
Gene namesi
Name:lysS
Ordered Locus Names:MG136
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Lysine--tRNA ligasePRO_0000152646Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi243273.MgenG_010200000955.

Structurei

3D structure databases

ProteinModelPortaliP47382.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1190.
KOiK04567.
OMAiMAQVADK.
OrthoDBiEOG69PQ2M.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47382-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDRLNDQAQ HRLQKLLRLK QTNNDPYLVT KTSLTHSSKS FQVEFEKCSE
60 70 80 90 100
EELKKKATVS LAGRIIAIRQ TFLIIQDFDG QVQLYINKKI HPKLFDYFNE
110 120 130 140 150
FVDIGDQIVV SGKPMLTKTK VLTLAVEEMK IIAKCLLVPP EKWHGLTDIE
160 170 180 190 200
TRARKRFLDL TYNLAMRDVF LKRTKIIKSI RSFLDQNGFI EVETPTLQAV
210 220 230 240 250
LGGANAKPFK THYNALKADF YLRIANEIAL KKLIIGGFNK VYEMGKMFRN
260 270 280 290 300
EGVDTTHNPE FTSIEIYQAY ADFEVMLVLV EKLIQSLCES LNQFSFNWNN
310 320 330 340 350
KTINLKTPFH KITMVELIKK VTGIDFNSVK DDQSAILLAE KHHVKLAKHQ
360 370 380 390 400
QNKQHIINLF FEQFCEQTLI EPTFVTHYPK AVSPLAKQDP SNPEFTQRFE
410 420 430 440 450
LFINGKEIAN AYSELNDPLE QRKRFEQQLE EKQLGNDETS ELDESFLEAL
460 470 480 490
SFGMVNTAGL GIGIDRLVML LCECNSIRDV VFFPQLREHK
Length:490
Mass (Da):56,565
Last modified:February 1, 1996 - v1
Checksum:i7F0BB0D76A0C13C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71353.1.
PIRiA64215.
RefSeqiNP_072798.1. NC_000908.2.
WP_009885692.1. NZ_AAGX01000002.1.

Genome annotation databases

EnsemblBacteriaiAAC71353; AAC71353; MG_136.
KEGGimge:MG_136.
PATRICi20009678. VBIMycGen98045_0148.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71353.1.
PIRiA64215.
RefSeqiNP_072798.1. NC_000908.2.
WP_009885692.1. NZ_AAGX01000002.1.

3D structure databases

ProteinModelPortaliP47382.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243273.MgenG_010200000955.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71353; AAC71353; MG_136.
KEGGimge:MG_136.
PATRICi20009678. VBIMycGen98045_0148.

Phylogenomic databases

eggNOGiCOG1190.
KOiK04567.
OMAiMAQVADK.
OrthoDBiEOG69PQ2M.

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-140-MONOMER.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiSYK_MYCGE
AccessioniPrimary (citable) accession number: P47382
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.