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P47368

- TOP1_MYCGE

UniProt

P47368 - TOP1_MYCGE

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Protein

DNA topoisomerase 1

Gene

topA

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1; catalyticUniRule annotation
Sitei34 – 341Interaction with DNAUniRule annotation
Metal bindingi95 – 951Magnesium 1; catalyticUniRule annotation
Metal bindingi95 – 951Magnesium 2UniRule annotation
Metal bindingi97 – 971Magnesium 2UniRule annotation
Sitei153 – 1531Interaction with DNAUniRule annotation
Sitei157 – 1571Interaction with DNAUniRule annotation
Active sitei334 – 3341O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei336 – 3361Interaction with DNAUniRule annotation
Sitei529 – 5291Interaction with DNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri618 – 64629C4-type 1Add
BLAST
Zinc fingeri667 – 69630C4-type 2Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-126-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:MG122
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 709709DNA topoisomerase 1PRO_0000145155Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243273.MG_122.

Structurei

3D structure databases

ProteinModelPortaliP47368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 127125ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni176 – 1816Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri618 – 64629C4-type 1Add
BLAST
Zinc fingeri667 – 69630C4-type 2Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
KOiK03168.
OMAiVVECDKC.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47368 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIKNLVVIES PNKVKTLKQY LPSDEFEIVS TVGHIREMVY KNFGFDENTY
60 70 80 90 100
TPIWEDWTKN KQKNPKQKHL LSKFEIIKSI KAKASDAQNI FLASDPDREG
110 120 130 140 150
EAISWHVYDL LDQKDKAKCK RITFNEITKK AVVDALKQPR NIDLNWVESQ
160 170 180 190 200
FARQILDRMI GFRLSRLLNS YLQAKSAGRV QSVALRFLEE REKEIAKFVP
210 220 230 240 250
RFWWTVDVLL NKENNQKVVC ANKSIPLVLR EINPELSASL KLDFEAAENV
260 270 280 290 300
SGIDFLNEAS ATRFANQLTG EYEVYFIDEP KIYYSSPNPV YTTASLQKDA
310 320 330 340 350
INKLGWSSKK VTMVAQRLYE GISVNGKQTA LISYPRTDSI RISNQFQSEC
360 370 380 390 400
EKYIEKEFGS HYLADKNKLK RHKKDEKIIQ DAHEGIHPTY ITITPNDLKN
410 420 430 440 450
GVKRDEFLLY RLIWIRTVAS LMADAKTSRT IVRFINQKNK FYTSSKSLLF
460 470 480 490 500
DGYQRLYEEI KPNTKDELYI DLSKLKIGDK FSFEKISVNE HKTNPPPRYT
510 520 530 540 550
QASLIEELEK SNIGRPSTYN TMASVNLERG YANLVNRFFY ITELGEKVNN
560 570 580 590 600
ELSKHFGNVI NKEFTKKMEK SLDEIAENKV NYQEFLKQFW TNFKSDVKLA
610 620 630 640 650
ENSIQKVKKE KELVERDCPK CNQPLVYRYT KRGNEKFVGC SDFPKCKYSE
660 670 680 690 700
FSNPKPKLTL ETLDELCPEC NNKLVKRRTK FNAKKTFIGC SNFPNCRFIK

KDNAAEFKQ
Length:709
Mass (Da):82,545
Last modified:February 1, 1996 - v1
Checksum:i1153189668672B5D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71340.1.
U02134 Genomic DNA. Translation: AAD12411.1.
U02242 Genomic DNA. Translation: AAA03398.1.
PIRiE64213.
RefSeqiNP_072784.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71340; AAC71340; MG_122.
GeneIDi875227.
KEGGimge:MG_122.
PATRICi20009648. VBIMycGen98045_0133.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71340.1 .
U02134 Genomic DNA. Translation: AAD12411.1 .
U02242 Genomic DNA. Translation: AAA03398.1 .
PIRi E64213.
RefSeqi NP_072784.1. NC_000908.2.

3D structure databases

ProteinModelPortali P47368.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243273.MG_122.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC71340 ; AAC71340 ; MG_122 .
GeneIDi 875227.
KEGGi mge:MG_122.
PATRICi 20009648. VBIMycGen98045_0133.

Phylogenomic databases

eggNOGi COG0551.
KOi K03168.
OMAi VVECDKC.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci MGEN243273:GH2R-126-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 399-481 AND 527-657.
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiTOP1_MYCGE
AccessioniPrimary (citable) accession number: P47368
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3