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P47368 (TOP1_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:MG122
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length709 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Ontologies

Keywords
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Magnesium
Metal-binding
Zinc
   Molecular functionIsomerase
Topoisomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA topological change

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentchromosome

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA topoisomerase type I activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 709709DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145155

Regions

Domain3 – 127125Toprim
Zinc finger618 – 64629C4-type 1 HAMAP-Rule MF_00952
Zinc finger667 – 69630C4-type 2 HAMAP-Rule MF_00952
Region176 – 1816Interaction with DNA By similarity

Sites

Active site3341O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding91Magnesium 1; catalytic By similarity
Metal binding951Magnesium 1; catalytic By similarity
Metal binding951Magnesium 2 By similarity
Metal binding971Magnesium 2 By similarity
Site341Interaction with DNA By similarity
Site1531Interaction with DNA By similarity
Site1571Interaction with DNA By similarity
Site3361Interaction with DNA By similarity
Site5291Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
P47368 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 1153189668672B5D

FASTA70982,545
        10         20         30         40         50         60 
MIKNLVVIES PNKVKTLKQY LPSDEFEIVS TVGHIREMVY KNFGFDENTY TPIWEDWTKN 

        70         80         90        100        110        120 
KQKNPKQKHL LSKFEIIKSI KAKASDAQNI FLASDPDREG EAISWHVYDL LDQKDKAKCK 

       130        140        150        160        170        180 
RITFNEITKK AVVDALKQPR NIDLNWVESQ FARQILDRMI GFRLSRLLNS YLQAKSAGRV 

       190        200        210        220        230        240 
QSVALRFLEE REKEIAKFVP RFWWTVDVLL NKENNQKVVC ANKSIPLVLR EINPELSASL 

       250        260        270        280        290        300 
KLDFEAAENV SGIDFLNEAS ATRFANQLTG EYEVYFIDEP KIYYSSPNPV YTTASLQKDA 

       310        320        330        340        350        360 
INKLGWSSKK VTMVAQRLYE GISVNGKQTA LISYPRTDSI RISNQFQSEC EKYIEKEFGS 

       370        380        390        400        410        420 
HYLADKNKLK RHKKDEKIIQ DAHEGIHPTY ITITPNDLKN GVKRDEFLLY RLIWIRTVAS 

       430        440        450        460        470        480 
LMADAKTSRT IVRFINQKNK FYTSSKSLLF DGYQRLYEEI KPNTKDELYI DLSKLKIGDK 

       490        500        510        520        530        540 
FSFEKISVNE HKTNPPPRYT QASLIEELEK SNIGRPSTYN TMASVNLERG YANLVNRFFY 

       550        560        570        580        590        600 
ITELGEKVNN ELSKHFGNVI NKEFTKKMEK SLDEIAENKV NYQEFLKQFW TNFKSDVKLA 

       610        620        630        640        650        660 
ENSIQKVKKE KELVERDCPK CNQPLVYRYT KRGNEKFVGC SDFPKCKYSE FSNPKPKLTL 

       670        680        690        700 
ETLDELCPEC NNKLVKRRTK FNAKKTFIGC SNFPNCRFIK KDNAAEFKQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC71340.1.
U02134 Genomic DNA. Translation: AAD12411.1.
U02242 Genomic DNA. Translation: AAA03398.1.
PIRE64213.
RefSeqNP_072784.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47368.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_122.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC71340; AAC71340; MG_122.
GeneID875227.
KEGGmge:MG_122.
PATRIC20009648. VBIMycGen98045_0133.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0551.
KOK03168.
OMAVVECDKC.
OrthoDBEOG6S7XQ9.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-126-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_MYCGE
AccessionPrimary (citable) accession number: P47368
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names