DNA topoisomerase 1 - Mycoplasma genitalium

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Reviewed, UniProtKB/Swiss-Prot P47368 (TOP1_MYCGE)

Last modified November 3, 2009. Version 65. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Ordered Locus Names:	MG122

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

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Protein attributes

Sequence length	709 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

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Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 709

709

DNA topoisomerase 1

PRO_0000145155

Regions

Zinc finger

618 – 646

C4-type 1

Zinc finger

667 – 696

C4-type 2

Sites

Active site

334

For DNA cleavage activity By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P47368-1 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 1153189668672B5D
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FASTA

709

82,545

        10         20         30         40         50         60 
MIKNLVVIES PNKVKTLKQY LPSDEFEIVS TVGHIREMVY KNFGFDENTY TPIWEDWTKN 

        70         80         90        100        110        120 
KQKNPKQKHL LSKFEIIKSI KAKASDAQNI FLASDPDREG EAISWHVYDL LDQKDKAKCK 

       130        140        150        160        170        180 
RITFNEITKK AVVDALKQPR NIDLNWVESQ FARQILDRMI GFRLSRLLNS YLQAKSAGRV 

       190        200        210        220        230        240 
QSVALRFLEE REKEIAKFVP RFWWTVDVLL NKENNQKVVC ANKSIPLVLR EINPELSASL 

       250        260        270        280        290        300 
KLDFEAAENV SGIDFLNEAS ATRFANQLTG EYEVYFIDEP KIYYSSPNPV YTTASLQKDA 

       310        320        330        340        350        360 
INKLGWSSKK VTMVAQRLYE GISVNGKQTA LISYPRTDSI RISNQFQSEC EKYIEKEFGS 

       370        380        390        400        410        420 
HYLADKNKLK RHKKDEKIIQ DAHEGIHPTY ITITPNDLKN GVKRDEFLLY RLIWIRTVAS 

       430        440        450        460        470        480 
LMADAKTSRT IVRFINQKNK FYTSSKSLLF DGYQRLYEEI KPNTKDELYI DLSKLKIGDK 

       490        500        510        520        530        540 
FSFEKISVNE HKTNPPPRYT QASLIEELEK SNIGRPSTYN TMASVNLERG YANLVNRFFY 

       550        560        570        580        590        600 
ITELGEKVNN ELSKHFGNVI NKEFTKKMEK SLDEIAENKV NYQEFLKQFW TNFKSDVKLA 

       610        620        630        640        650        660 
ENSIQKVKKE KELVERDCPK CNQPLVYRYT KRGNEKFVGC SDFPKCKYSE FSNPKPKLTL 

       670        680        690        700 
ETLDELCPEC NNKLVKRRTK FNAKKTFIGC SNFPNCRFIK KDNAAEFKQ

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 399-481 AND 527-657. Strain: ATCC 33530 / G-37 / NCTC 10195.

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Cross-references

Sequence databases
	L43967 Genomic DNA. Translation: AAC71340.1. U02134 Genomic DNA. Translation: AAD12411.1. U02242 Genomic DNA. Translation: AAA03398.1.
PIR	E64213.
RefSeq	NP_072784.1.
3D structure databases
HSSP	HSSP built from PDB template 1ECL based on UniProtKB P06612.
ModBase	Search...
Genome annotation databases
GeneID	875227.
GenomeReviews	Gene locus MG122 in contig L43967_GR.
KEGG	mge:MG_122.
TIGR	MG122.
Phylogenomic databases
HOGENOM	P47368.
OMA	KCKRITF.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_122-MON.
BRENDA	5.99.1.2. 110.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01051. topA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

TOP1_MYCGE

Accession

Primary (citable) accession number: P47368

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents