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P47368

- TOP1_MYCGE

UniProt

P47368 - TOP1_MYCGE

Protein

DNA topoisomerase 1

Gene

topA

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91Magnesium 1; catalyticUniRule annotation
    Sitei34 – 341Interaction with DNAUniRule annotation
    Metal bindingi95 – 951Magnesium 1; catalyticUniRule annotation
    Metal bindingi95 – 951Magnesium 2UniRule annotation
    Metal bindingi97 – 971Magnesium 2UniRule annotation
    Sitei153 – 1531Interaction with DNAUniRule annotation
    Sitei157 – 1571Interaction with DNAUniRule annotation
    Active sitei334 – 3341O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei336 – 3361Interaction with DNAUniRule annotation
    Sitei529 – 5291Interaction with DNAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri618 – 64629C4-type 1Add
    BLAST
    Zinc fingeri667 – 69630C4-type 2Add
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMGEN243273:GH2R-126-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:MG122
    OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
    Taxonomic identifieri243273 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000807: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 709709DNA topoisomerase 1PRO_0000145155Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi243273.MG_122.

    Structurei

    3D structure databases

    ProteinModelPortaliP47368.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 127125ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni176 – 1816Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri618 – 64629C4-type 1Add
    BLAST
    Zinc fingeri667 – 69630C4-type 2Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0551.
    KOiK03168.
    OMAiVVECDKC.
    OrthoDBiEOG6S7XQ9.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 2 hits.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47368-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIKNLVVIES PNKVKTLKQY LPSDEFEIVS TVGHIREMVY KNFGFDENTY    50
    TPIWEDWTKN KQKNPKQKHL LSKFEIIKSI KAKASDAQNI FLASDPDREG 100
    EAISWHVYDL LDQKDKAKCK RITFNEITKK AVVDALKQPR NIDLNWVESQ 150
    FARQILDRMI GFRLSRLLNS YLQAKSAGRV QSVALRFLEE REKEIAKFVP 200
    RFWWTVDVLL NKENNQKVVC ANKSIPLVLR EINPELSASL KLDFEAAENV 250
    SGIDFLNEAS ATRFANQLTG EYEVYFIDEP KIYYSSPNPV YTTASLQKDA 300
    INKLGWSSKK VTMVAQRLYE GISVNGKQTA LISYPRTDSI RISNQFQSEC 350
    EKYIEKEFGS HYLADKNKLK RHKKDEKIIQ DAHEGIHPTY ITITPNDLKN 400
    GVKRDEFLLY RLIWIRTVAS LMADAKTSRT IVRFINQKNK FYTSSKSLLF 450
    DGYQRLYEEI KPNTKDELYI DLSKLKIGDK FSFEKISVNE HKTNPPPRYT 500
    QASLIEELEK SNIGRPSTYN TMASVNLERG YANLVNRFFY ITELGEKVNN 550
    ELSKHFGNVI NKEFTKKMEK SLDEIAENKV NYQEFLKQFW TNFKSDVKLA 600
    ENSIQKVKKE KELVERDCPK CNQPLVYRYT KRGNEKFVGC SDFPKCKYSE 650
    FSNPKPKLTL ETLDELCPEC NNKLVKRRTK FNAKKTFIGC SNFPNCRFIK 700
    KDNAAEFKQ 709
    Length:709
    Mass (Da):82,545
    Last modified:February 1, 1996 - v1
    Checksum:i1153189668672B5D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71340.1.
    U02134 Genomic DNA. Translation: AAD12411.1.
    U02242 Genomic DNA. Translation: AAA03398.1.
    PIRiE64213.
    RefSeqiNP_072784.1. NC_000908.2.

    Genome annotation databases

    EnsemblBacteriaiAAC71340; AAC71340; MG_122.
    GeneIDi875227.
    KEGGimge:MG_122.
    PATRICi20009648. VBIMycGen98045_0133.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71340.1 .
    U02134 Genomic DNA. Translation: AAD12411.1 .
    U02242 Genomic DNA. Translation: AAA03398.1 .
    PIRi E64213.
    RefSeqi NP_072784.1. NC_000908.2.

    3D structure databases

    ProteinModelPortali P47368.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243273.MG_122.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC71340 ; AAC71340 ; MG_122 .
    GeneIDi 875227.
    KEGGi mge:MG_122.
    PATRICi 20009648. VBIMycGen98045_0133.

    Phylogenomic databases

    eggNOGi COG0551.
    KOi K03168.
    OMAi VVECDKC.
    OrthoDBi EOG6S7XQ9.

    Enzyme and pathway databases

    BioCyci MGEN243273:GH2R-126-MONOMER.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 2 hits.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33530 / G-37 / NCTC 10195.
    2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
      Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
      J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 399-481 AND 527-657.
      Strain: ATCC 33530 / G-37 / NCTC 10195.

    Entry informationi

    Entry nameiTOP1_MYCGE
    AccessioniPrimary (citable) accession number: P47368
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Mycoplasma genitalium
      Mycoplasma genitalium (strain G-37): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3