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Protein

DNA topoisomerase 1

Gene

topA

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9Magnesium 1; catalyticUniRule annotation1
Metal bindingi95Magnesium 1; catalyticUniRule annotation1
Metal bindingi95Magnesium 2UniRule annotation1
Metal bindingi97Magnesium 2UniRule annotation1
Active sitei334O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri618 – 646C4-type 1Add BLAST29
Zinc fingeri667 – 696C4-type 2Add BLAST30

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMGEN243273:G1GJ2-135-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:MG122
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451551 – 709DNA topoisomerase 1Add BLAST709

Proteomic databases

PRIDEiP47368

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei34Interaction with DNAUniRule annotation1
Sitei153Interaction with DNAUniRule annotation1
Sitei157Interaction with DNAUniRule annotation1
Sitei336Interaction with DNAUniRule annotation1
Sitei529Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi243273.MgenG_010200000865

Structurei

3D structure databases

ProteinModelPortaliP47368
SMRiP47368
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 127ToprimUniRule annotationAdd BLAST125

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni176 – 181Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri618 – 646C4-type 1Add BLAST29
Zinc fingeri667 – 696C4-type 2Add BLAST30

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG4105C73 Bacteria
COG0550 LUCA
COG0551 LUCA
KOiK03168
OMAiPECKYTR
OrthoDBiPOG091H02BN

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03363 TOPRIM_TopoIA_TopoI, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
HAMAPiMF_00952 Topoisom_1_prok, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR013498 Topo_IA_Znf
IPR005733 TopoI_bac-type
IPR028612 Topoisom_1_IA
IPR006171 TOPRIM_domain
IPR034149 TOPRIM_TopoI
PANTHERiPTHR42785 PTHR42785, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PF01396 zf-C4_Topoisom, 2 hits
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
TIGRFAMsiTIGR01051 topA_bact, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

P47368-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKNLVVIES PNKVKTLKQY LPSDEFEIVS TVGHIREMVY KNFGFDENTY
60 70 80 90 100
TPIWEDWTKN KQKNPKQKHL LSKFEIIKSI KAKASDAQNI FLASDPDREG
110 120 130 140 150
EAISWHVYDL LDQKDKAKCK RITFNEITKK AVVDALKQPR NIDLNWVESQ
160 170 180 190 200
FARQILDRMI GFRLSRLLNS YLQAKSAGRV QSVALRFLEE REKEIAKFVP
210 220 230 240 250
RFWWTVDVLL NKENNQKVVC ANKSIPLVLR EINPELSASL KLDFEAAENV
260 270 280 290 300
SGIDFLNEAS ATRFANQLTG EYEVYFIDEP KIYYSSPNPV YTTASLQKDA
310 320 330 340 350
INKLGWSSKK VTMVAQRLYE GISVNGKQTA LISYPRTDSI RISNQFQSEC
360 370 380 390 400
EKYIEKEFGS HYLADKNKLK RHKKDEKIIQ DAHEGIHPTY ITITPNDLKN
410 420 430 440 450
GVKRDEFLLY RLIWIRTVAS LMADAKTSRT IVRFINQKNK FYTSSKSLLF
460 470 480 490 500
DGYQRLYEEI KPNTKDELYI DLSKLKIGDK FSFEKISVNE HKTNPPPRYT
510 520 530 540 550
QASLIEELEK SNIGRPSTYN TMASVNLERG YANLVNRFFY ITELGEKVNN
560 570 580 590 600
ELSKHFGNVI NKEFTKKMEK SLDEIAENKV NYQEFLKQFW TNFKSDVKLA
610 620 630 640 650
ENSIQKVKKE KELVERDCPK CNQPLVYRYT KRGNEKFVGC SDFPKCKYSE
660 670 680 690 700
FSNPKPKLTL ETLDELCPEC NNKLVKRRTK FNAKKTFIGC SNFPNCRFIK

KDNAAEFKQ
Length:709
Mass (Da):82,545
Last modified:February 1, 1996 - v1
Checksum:i1153189668672B5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA Translation: AAC71340.1
U02134 Genomic DNA Translation: AAD12411.1
U02242 Genomic DNA Translation: AAA03398.1
PIRiE64213
RefSeqiWP_009885678.1, NZ_AAGX01000002.1

Genome annotation databases

EnsemblBacteriaiAAC71340; AAC71340; MG_122
KEGGimge:MG_122

Similar proteinsi

Entry informationi

Entry nameiTOP1_MYCGE
AccessioniPrimary (citable) accession number: P47368
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
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