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Protein

Peptide deformylase

Gene

def

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions (By similarity).By similarity

Catalytic activityi

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.

Cofactori

Fe2+By similarityNote: Binds 1 Fe2+ ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi111IronBy similarity1
Metal bindingi155IronBy similarity1
Active sitei156By similarity1
Metal bindingi159IronBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processProtein biosynthesis
LigandIron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide deformylase (EC:3.5.1.88)
Short name:
PDF
Alternative name(s):
Polypeptide deformylase
Gene namesi
Name:def
Ordered Locus Names:MG106
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Probably essential, it was not disrupted in a global transposon mutagenesis study.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000828011 – 193Peptide deformylaseAdd BLAST193

Interactioni

Protein-protein interaction databases

STRINGi243273.MgenG_010200000775

Structurei

3D structure databases

ProteinModelPortaliP47352
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polypeptide deformylase family.Curated

Phylogenomic databases

eggNOGiENOG4107YB9 Bacteria
COG0242 LUCA
KOiK01462
OMAiMILMKDI
OrthoDBiPOG091H02B0

Family and domain databases

CDDicd00487 Pep_deformylase, 1 hit
Gene3Di3.90.45.10, 1 hit
HAMAPiMF_00163 Pep_deformylase, 1 hit
InterProiView protein in InterPro
IPR023635 Peptide_deformylase
IPR036821 Peptide_deformylase_sf
PANTHERiPTHR10458 PTHR10458, 1 hit
PfamiView protein in Pfam
PF01327 Pep_deformylase, 1 hit
PIRSFiPIRSF004749 Pep_def, 1 hit
PRINTSiPR01576 PDEFORMYLASE
SUPFAMiSSF56420 SSF56420, 1 hit
TIGRFAMsiTIGR00079 pept_deformyl, 1 hit

Sequencei

Sequence statusi: Complete.

P47352-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFQPTKTWL VFDDNALINK PTEAVNFPID EQIETCIKKM IAYVDASYDG
60 70 80 90 100
KAQEYDIIPG IGIAANQIGY WKQLFYIHLN DLNKEKKCLL INPKIIDQSE
110 120 130 140 150
NKAFLESGEG CLSVKKQHKG YVIRSEWITI KGYDWFEKKE ITIKATGLFG
160 170 180 190
MCLQHEFDHL QGRFFYQRIN PLNPWFKKPE WKVINPTLKT SNG
Length:193
Mass (Da):22,470
Last modified:January 31, 2018 - v3
Checksum:i3662B5A8064A0307
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA Translation: AAC71324.2
RefSeqiWP_009885664.1, NZ_AAGX01000002.1

Genome annotation databases

EnsemblBacteriaiAAC71324; AAC71324; MG_106
KEGGimge:MG_106

Similar proteinsi

Entry informationi

Entry nameiDEF_MYCGE
AccessioniPrimary (citable) accession number: P47352
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 31, 2018
Last modified: March 28, 2018
This is version 111 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health