Reviewed, UniProtKB/Swiss-Prot P47348 (TRXB_MYCGE)

Names and origin

Protein names

Recommended name:
    Thioredoxin reductase
      Short name=TRXR
    EC=1.8.1.9

Gene names

Name:	trxB
Ordered Locus Names:	MG102

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW removal of superoxide radicals Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Thioredoxin reductase		PRO_0000166737
Regions
	Nucleotide binding	45 – 52	8	FAD By similarity
	Nucleotide binding	288 – 297	10	FAD By similarity
Amino acid modifications
	Disulfide bond	145 ↔ 148		Redox-active By similarity
Experimental info
	Sequence conflict	113 – 122	10	VFSKTVIYAT → FLAKLLSMQQ Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47348-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 7FDF8FF755C9D424	FASTA	315	34,674
	10 20 30 40 50 60 MLKVNADFLT KDQVIYDLVI VGAGPAGIAS AIYGKRANLN LAIIEGNTPG GKIVKTNIVE 70 80 90 100 110 120 NYPGFKTITG PELGLEMYNH LLAFEPVVFY NNLIKIDHLN DTFILYLDNK TTVFSKTVIY 130 140 150 160 170 180 ATGMEERKLG IEKEDYFYGK GISYCAICDA ALYKGKTVGV VGGGNSAIQE AIYLSSIAKT 190 200 210 220 230 240 VHLIHRREVF RSDALLVEKL KKISNVVFHL NATVKQLIGQ EKLQTVKLAS TVDKSESEIA 250 260 270 280 290 300 IDCLFPYIGF ESNNKPVLDL KLNLDQNGFI LGDENMQTNI KGFYVAGDCR SKSFRQIATA 310 ISDGVTAVLK VRDDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 16-122. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43967 Genomic DNA. Translation: AAC71320.1. U02197 Genomic DNA. Translation: AAD12483.1.
PIR	C64211.
RefSeq	NP_072764.1.
3D structure databases
HSSP	HSSP built from PDB template 1TRB based on UniProtKB P09625.
ModBase	Search...
Genome annotation databases
GeneID	875416.
GenomeReviews	Gene locus MG102 in contig L43967_GR.
KEGG	mge:MG_102.
TIGR	MG102.
Phylogenomic databases
HOGENOM	P47348.
OMA	KVTMLVR.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_102-MON.
BRENDA	1.8.1.9. 110.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR005982. Thioredox_Rdtase. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01292. TRX_reduct. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXB_MYCGE

Accession

Primary (citable) accession number: P47348
Secondary accession number(s): Q49316

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)