P47346 (GATB_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-

Gene names

Name:	gatB
Ordered Locus Names:	MG100

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00121
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00121 ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP-Rule MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP-Rule MF_00121
Sequence similarities	Belongs to the GatB/GatE family. GatB subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-nitrogen ligase activity, with glutamine as amido-N-donor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP-Rule MF_00121		PRO_0000148807

Sequences

	Sequence		Length	Mass (Da)
	P47346 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: E8A05FA097913D96	FASTA	477	54,695
	10 20 30 40 50 60 MINFEAIIGI EVHVVLNTAS KMFSKAPNRV DNQKINHFID PIDLGLPGTL PQVNELAVYK 70 80 90 100 110 120 ALLLADALKM KTVTNKLVFD RKHYFYQDLP KGFQITQQNY PFAKNGVVTI NVDAIEKPIY 130 140 150 160 170 180 IDRFHLEEDT AKQHFNHDQI LLDFNRCGAP LIEVVTLPVI NTAKEAKAYL QKLRQILIVN 190 200 210 220 230 240 NISNAKLEDG SMRSDCNVSV RLKGQRQLGT KIEIKNINSL NNVEKAIDLE INRQVKALIN 250 260 270 280 290 300 GETLSQATLS FDDKTNNNVF MRKKDNTIDY RYFIEPNIMT SNIDDLLLEK PVAFQLEQFQ 310 320 330 340 350 360 KELIDSNVNP QLVQLVVDDA TIFSAFQTIN SVIKNPQETI RWLCIELIGQ LNKTNSSLTA 370 380 390 400 410 420 NLIQDLITLI EMLKAAKVNQ KQAKQLITLM IETKKDPKSL AKLHNLEQIT DPKELQKIIK 430 440 450 460 470 KIFQENEKEI LKNIDRIERI QKLIIGQVMH KTNNRANPQQ VFIIVENMLH EVRERDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 80-177. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71318.1. U01799 Genomic DNA. Translation: AAD12325.1.
PIR	A64211.
RefSeq	NP_072762.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47346.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_100.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71318; AAC71318; MG_100.
GeneID	875247.
KEGG	mge:MG_100.
PATRIC	20009602. VBIMycGen98045_0110.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0064.
KO	K02434.
OMA	QDLPKGF.
ProtClustDB	PRK05477.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-103-MONOMER.
Family and domain databases
HAMAP	MF_00121. GatB.
InterPro	IPR004413. Apn/Gln-ADT_bsu. IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
PANTHER	PTHR11659. PTHR11659. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. gatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATB_MYCGE

Accession

Primary (citable) accession number: P47346

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program