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P47346 (GATB_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-
Gene names
Name:gatB
Ordered Locus Names:MG100
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00121

Catalytic activity

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00121

ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP-Rule MF_00121

Subunit structure

Heterotrimer of A, B and C subunits By similarity. HAMAP-Rule MF_00121

Sequence similarities

Belongs to the GatB/GatE family. GatB subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

carbon-nitrogen ligase activity, with glutamine as amido-N-donor

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 477477Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP-Rule MF_00121
PRO_0000148807

Sequences

Sequence LengthMass (Da)Tools
P47346 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: E8A05FA097913D96

FASTA47754,695
        10         20         30         40         50         60 
MINFEAIIGI EVHVVLNTAS KMFSKAPNRV DNQKINHFID PIDLGLPGTL PQVNELAVYK 

        70         80         90        100        110        120 
ALLLADALKM KTVTNKLVFD RKHYFYQDLP KGFQITQQNY PFAKNGVVTI NVDAIEKPIY 

       130        140        150        160        170        180 
IDRFHLEEDT AKQHFNHDQI LLDFNRCGAP LIEVVTLPVI NTAKEAKAYL QKLRQILIVN 

       190        200        210        220        230        240 
NISNAKLEDG SMRSDCNVSV RLKGQRQLGT KIEIKNINSL NNVEKAIDLE INRQVKALIN 

       250        260        270        280        290        300 
GETLSQATLS FDDKTNNNVF MRKKDNTIDY RYFIEPNIMT SNIDDLLLEK PVAFQLEQFQ 

       310        320        330        340        350        360 
KELIDSNVNP QLVQLVVDDA TIFSAFQTIN SVIKNPQETI RWLCIELIGQ LNKTNSSLTA 

       370        380        390        400        410        420 
NLIQDLITLI EMLKAAKVNQ KQAKQLITLM IETKKDPKSL AKLHNLEQIT DPKELQKIIK 

       430        440        450        460        470 
KIFQENEKEI LKNIDRIERI QKLIIGQVMH KTNNRANPQQ VFIIVENMLH EVRERDS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC71318.1.
U01799 Genomic DNA. Translation: AAD12325.1.
PIRA64211.
RefSeqNP_072762.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47346.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_100.

Proteomic databases

PRIDEP47346.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC71318; AAC71318; MG_100.
GeneID875247.
KEGGmge:MG_100.
PATRIC20009602. VBIMycGen98045_0110.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0064.
KOK02434.
OMAAIDYEES.
OrthoDBEOG6RJV5B.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-103-MONOMER.

Family and domain databases

HAMAPMF_00121. GatB.
InterProIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERPTHR11659. PTHR11659. 1 hit.
PfamPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMSSF89095. SSF89095. 1 hit.
TIGRFAMsTIGR00133. gatB. 1 hit.
PROSITEPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGATB_MYCGE
AccessionPrimary (citable) accession number: P47346
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names