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Protein

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Gene

gatB

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity).By similarity

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.
ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-103-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (EC:6.3.5.-)
Short name:
Asp/Glu-ADT subunit B
Gene namesi
Name:gatB
Ordered Locus Names:MG100
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BPRO_0000148807Add
BLAST

Proteomic databases

PRIDEiP47346.

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.By similarity

Protein-protein interaction databases

STRINGi243273.MG_100.

Structurei

3D structure databases

ProteinModelPortaliP47346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatB subfamily.Curated

Phylogenomic databases

eggNOGiCOG0064.
KOiK02434.
OMAiMINFEAI.
OrthoDBiEOG6RJV5B.

Family and domain databases

HAMAPiMF_00121. GatB.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47346-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MINFEAIIGI EVHVVLNTAS KMFSKAPNRV DNQKINHFID PIDLGLPGTL
60 70 80 90 100
PQVNELAVYK ALLLADALKM KTVTNKLVFD RKHYFYQDLP KGFQITQQNY
110 120 130 140 150
PFAKNGVVTI NVDAIEKPIY IDRFHLEEDT AKQHFNHDQI LLDFNRCGAP
160 170 180 190 200
LIEVVTLPVI NTAKEAKAYL QKLRQILIVN NISNAKLEDG SMRSDCNVSV
210 220 230 240 250
RLKGQRQLGT KIEIKNINSL NNVEKAIDLE INRQVKALIN GETLSQATLS
260 270 280 290 300
FDDKTNNNVF MRKKDNTIDY RYFIEPNIMT SNIDDLLLEK PVAFQLEQFQ
310 320 330 340 350
KELIDSNVNP QLVQLVVDDA TIFSAFQTIN SVIKNPQETI RWLCIELIGQ
360 370 380 390 400
LNKTNSSLTA NLIQDLITLI EMLKAAKVNQ KQAKQLITLM IETKKDPKSL
410 420 430 440 450
AKLHNLEQIT DPKELQKIIK KIFQENEKEI LKNIDRIERI QKLIIGQVMH
460 470
KTNNRANPQQ VFIIVENMLH EVRERDS
Length:477
Mass (Da):54,695
Last modified:February 1, 1996 - v1
Checksum:iE8A05FA097913D96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71318.1.
U01799 Genomic DNA. Translation: AAD12325.1.
PIRiA64211.
RefSeqiNP_072762.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71318; AAC71318; MG_100.
GeneIDi875247.
KEGGimge:MG_100.
PATRICi20009602. VBIMycGen98045_0110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71318.1.
U01799 Genomic DNA. Translation: AAD12325.1.
PIRiA64211.
RefSeqiNP_072762.1. NC_000908.2.

3D structure databases

ProteinModelPortaliP47346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243273.MG_100.

Proteomic databases

PRIDEiP47346.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71318; AAC71318; MG_100.
GeneIDi875247.
KEGGimge:MG_100.
PATRICi20009602. VBIMycGen98045_0110.

Phylogenomic databases

eggNOGiCOG0064.
KOiK02434.
OMAiMINFEAI.
OrthoDBiEOG6RJV5B.

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-103-MONOMER.

Family and domain databases

HAMAPiMF_00121. GatB.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 80-177.
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiGATB_MYCGE
AccessioniPrimary (citable) accession number: P47346
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 4, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.