UvrABC system protein B - Mycoplasma genitalium

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Reviewed, UniProtKB/Swiss-Prot P47319 (UVRB_MYCGE)

Last modified November 24, 2009. Version 65. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    UvrABC system protein B
      Short name=Protein uvrB
Alternative name(s):
    Excinuclease ABC subunit B

Gene names

Name:	uvrB
Ordered Locus Names:	MG073

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

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Protein attributes

Sequence length	656 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon binding of the uvrA₂B₂ complex to a putative damaged site, the DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP binding by uvrB and probably causes local melting of the DNA helix, facilitating insertion of uvrB beta-hairpin between the DNA strands. Then uvrB probes one DNA strand for the presence of a lesion. If a lesion is found the uvrA subunits dissociate and the uvrB-DNA preincision complex is formed. This complex is subsequently bound by uvrC and the second uvrB is released. If no lesion is found, the DNA wraps around the other uvrB subunit that will check the other stand for damage By similarity.
Subunit structure	Forms a heterotetramer with uvrA during the search for lesions. Interacts with uvrC in an incision complex By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	The beta-hairpin motif is involved in DNA binding By similarity.
Sequence similarities	Belongs to the uvrB family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 UVR domain.

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Ontologies

Keywords
Biological process	DNA damage DNA excision DNA repair SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Excision nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	SOS response Inferred from electronic annotation. Source: HAMAP nucleotide-excision repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP excinuclease ABC activity Inferred from electronic annotation. Source: HAMAP helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 656

656

UvrABC system protein B HAMAP MF_00204

PRO_0000138407

Regions

Domain

29 – 414

386

Helicase ATP-binding

Domain

434 – 596

163

Helicase C-terminal

Domain

614 – 649

UVR

Nucleotide binding

42 – 49

ATP Potential

Motif

95 – 118

Beta-hairpin HAMAP MF_00204

Experimental info

Sequence conflict

G → A Ref.2

Sequence conflict

102 – 107

EAYLPS → KLTYPD in AAD12393. Ref.2

Sequence conflict

432 – 433

DN → IT Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

P47319-1 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 59488ADF5D283493
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FASTA

656

75,202

        10         20         30         40         50         60 
MKNQTKSNSL FQLSTNYIPT GDQPEAIKKL SEFKTKQQVL LGATGTGKTF TIANVIQNSQ 

        70         80         90        100        110        120 
LPTVVIAHNK TLAGQLFNEL KQLFPKNAVE YFISYFDFYQ PEAYLPSKGI YIEKSATVNE 

       130        140        150        160        170        180 
AIKRLRVSTL HSLSTRKDVI VVGSVASIYP TSSPSDFVKY CLWFVVGKDY DLKTIKDRLV 

       190        200        210        220        230        240 
SLNYVVNKQQ LTPGKFRFQG DVLEVFPGYS DAFVIRISFF DTKVEQICQI DPLTNKILNQ 

       250        260        270        280        290        300 
LFEIKIGPAD EYVVNQSDLD IAIKNIKQEL QERVNYFNKQ NLVERAQRLA TITNHDLNDL 

       310        320        330        340        350        360 
KAWGFCSGVE NYARHLELRM ANSTPYSIFD YFKGDWLLVI DESHQTLPQL NGMYNTDLSR 

       370        380        390        400        410        420 
KQSLIDYGFR LPSALDNRPL SFAELQQKMQ KVIYVSATPR DKEISLSQNN VIEQLVRPTY 

       430        440        450        460        470        480 
LVDPIIVVKP KDNQVEDLIE EIINQRQNNT RTFVTVLTIK MAENLTEYLK ERKIKVAYIH 

       490        500        510        520        530        540 
KDIKALERLL LINDLRRGEY ECLVGINLLR EGLDVPEVAL VCIFDADIPG LPRDERSLIQ 

       550        560        570        580        590        600 
IIGRAARNEH GRVVMYANHV TEQMQKAIDE TKRRRTVQME YNKLHNKTPK TVVKPLTFVQ 

       610        620        630        640        650 
PIKLKAKSNA EKNAALIKQL TKEMKKAAAN QNYELAIEIR DSIFELEKEI GSKIKV

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-78; 102-231 AND 414-528. Strain: ATCC 33530 / G-37 / NCTC 10195.

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Cross-references

Sequence databases
	L43967 Genomic DNA. Translation: AAC71291.1. U01743 Genomic DNA. Translation: AAD10554.1. Different initiation. U02119 Genomic DNA. Translation: AAD12393.1. U01698 Genomic DNA. Translation: AAB01010.1.
PIR	A64208.
RefSeq	NP_072735.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	875513.
GenomeReviews	Gene locus MG073 in contig L43967_GR.
KEGG	mge:MG_073.
TIGR	MG073.
Phylogenomic databases
HOGENOM	P47319.
OMA	CNGVENY
Enzyme and pathway databases
BioCyc	MGEN243273:MG_073-MON.
Family and domain databases
HAMAP	MF_00204. [Tree]
InterPro	IPR014001. DEAD-like_N. IPR001650. DNA/RNA_helicase_C. IPR014021. Helicase_SF1/SF2_ATP-bd. IPR006935. Restrct_endonuc_I_R/III_Res. IPR001943. UvrB/C. IPR004807. UvrB_ABC. IPR009055. UvrB_UvrC-bd_C. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF04851. ResIII. 1 hit. PF02151. UVR. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00631. uvrb. 1 hit.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS50151. UVR. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

UVRB_MYCGE

Accession

Primary (citable) accession number: P47319
Secondary accession number(s): Q49254, Q49446, Q49506

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 24, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents