Reviewed, UniProtKB/Swiss-Prot P47318 (SECA_MYCGE)

Names and origin

Protein names

Recommended name:
    Protein translocase subunit secA

Gene names

Name:	secA
Ordered Locus Names:	MG072

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	806 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of the Sec protein translocase complex. Interacts with the secYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane By similarity.
Subunit structure	Monomer and homodimer By similarity. Part of the essential Sec protein translocation apparatus which comprises secA, secYEG and auxiliary proteins secDF. Other proteins may also be involved By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm By similarity. Note: Distribution is 50-50 By similarity.
Sequence similarities	Belongs to the secA family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Cell membrane Cytoplasm Membrane
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intracellular protein transmembrane transport Inferred from electronic annotation. Source: HAMAP protein import Inferred from electronic annotation. Source: InterPro protein targeting Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 806	806	Protein translocase subunit secA HAMAP MF_01382		PRO_0000109593
Regions
	Nucleotide binding	102 – 109	8	ATP Potential
Experimental info
	Sequence conflict	494 – 497	4	IRLG → YPFS in AAD10541. Ref.2
	Sequence conflict	560	1	S → T in AAD10541. Ref.2
	Sequence conflict	610 – 618	9	KQRDKFLLA → TREVFIS in AAD10541. Ref.2
	Sequence conflict	624 – 625	2	MI → WS in AAD10541. Ref.2
	Sequence conflict	629	1	L → H in AAD10541. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47318-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: FDD495AF98B1320F	FASTA	806	91,585
	10 20 30 40 50 60 MAIFNFLKLI SPKNRILSKA NRIASEVESY KNYYRNLTDQ QLFEESNKLV DLVTKQNYTI 70 80 90 100 110 120 LDVCVAALAL IREVVYRETG EFAYRVQIIG AFIVLSGDFA EMMTGEGKTL TIVLAAYVSA 130 140 150 160 170 180 LEKRGVHVVT VNEYLAQRDA NNAMKILKRV GMSVGCNFAN LSPQLKQAAF NCDVTYTTNS 190 200 210 220 230 240 ELGFDYLRDN MVHSYQDKKI RELHFAIVDE GDSVLIDEAR TPLIISGPSK NEFGLYVAVD 250 260 270 280 290 300 RFVKSLTEQE FKIDPESRAA SLTELGIKKA EQTFKKENLF ALENSDLFHK IMNGLTAVKV 310 320 330 340 350 360 FEQGKEYIVR DGKVLIVDHF TGRILEGRSY SNGLQQAVQA KEYVEIEPEN VIVATITYQS 370 380 390 400 410 420 FFRLYNRLAA VSGTALTESE EFLKIYNMVV VPVPTNRPNI RKDRSDSVFG TPQIKWMAVV 430 440 450 460 470 480 KEIKKIHETS RPILIGTANI DDSELLHNLL LEANIPHEVL NAKNHSREAE IVTKAGQKNA 490 500 510 520 530 540 VTISTNMAGR GTDIRLGEGV AEMGGLYVLG TERNESRRID NQLRGRAARQ GDKGETKFFI 550 560 570 580 590 600 SLGDSLFKRF AHDKIERAIS KLGNETFDSA FFSKMLSRTQ KRVEAINFDT RKNLIDYDHV 610 620 630 640 650 660 LASQRELIYK QRDKFLLAND LSEMIDKMLE KFVQQFCDQY RNQKNQNLIN HIALAEALNL 670 680 690 700 710 720 EMNMQNTINP KVFENMTFDV AVDKTRNLVA KKISDKVNVL TKPIALNRFR DIIITSMDKH 730 740 750 760 770 780 WTEHLDSVFK LREGVVLRSM EHTSPLNVYI KETDILFKTM LQKIAQDVIV QIANLTTPDE 790 800 FDHSLMQANA LKKLAAIKAD EKSNQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 494-702 AND 757-806. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43967 Genomic DNA. Translation: AAC71290.1. U01732 Genomic DNA. Translation: AAD10541.1. U01743 Genomic DNA. Translation: AAD10553.1.
PIR	I64207.
RefSeq	NP_072734.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	875509.
KEGG	mge:MG_072.
TIGR	MG072.
Phylogenomic databases
HOGENOM	HBG284200.
OMA	EANIPHE.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_072-MON.
Family and domain databases
HAMAP	MF_01382. [Tree]
InterPro	IPR001650. DNA/RNA_helicase_C. IPR000185. SecA. IPR011115. SecA_DEAD. IPR014018. SecA_motor_DEAD. IPR011130. SecA_PP_bd. IPR011116. SecA_Wing/Scaffold. [Graphical view]
Gene3D	G3DSA:1.10.3060.10. SecA_SW. 1 hit.
Pfam	PF00271. Helicase_C. 1 hit. PF07517. SecA_DEAD. 1 hit. PF01043. SecA_PP_bind. 1 hit. PF07516. SecA_SW. 1 hit. [Graphical view]
PRINTS	PR00906. SECA.
PROSITE	PS01312. SECA. 1 hit. PS51196. SECA_MOTOR_DEAD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SECA_MYCGE

Accession

Primary (citable) accession number: P47318
Secondary accession number(s): Q49438

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 15, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)