SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P47315

- PTG3C_MYCGE

UniProt

P47315 - PTG3C_MYCGE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
PTS system glucose-specific EIICBA component
Gene
ptsG, MG069
Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport.

Catalytic activityi

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei653 – 6531Phosphocysteine intermediate; for EIIB activity By similarity
Active sitei815 – 8151Tele-phosphohistidine intermediate; for EIIA activity By similarity

GO - Molecular functioni

  1. kinase activity Source: UniProtKB-KW
  2. protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-72-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system glucose-specific EIICBA component
Alternative name(s):
EII-Glc/EIII-Glc
EIICBA-Glc
Including the following 3 domains:
Glucose permease IIC component
Alternative name(s):
PTS system glucose-specific EIIC component
Glucose-specific phosphotransferase enzyme IIB component (EC:2.7.1.69)
Alternative name(s):
PTS system glucose-specific EIIB component
Glucose-specific phosphotransferase enzyme IIA component (EC:2.7.1.-)
Alternative name(s):
PTS system glucose-specific EIIA component
Gene namesi
Name:ptsG
Ordered Locus Names:MG069
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei31 – 5121Helical; Reviewed prediction
Add
BLAST
Transmembranei71 – 9121Helical; Reviewed prediction
Add
BLAST
Transmembranei100 – 12021Helical; Reviewed prediction
Add
BLAST
Transmembranei155 – 17521Helical; Reviewed prediction
Add
BLAST
Transmembranei189 – 20921Helical; Reviewed prediction
Add
BLAST
Transmembranei459 – 47921Helical; Reviewed prediction
Add
BLAST
Transmembranei487 – 50721Helical; Reviewed prediction
Add
BLAST
Transmembranei509 – 52921Helical; Reviewed prediction
Add
BLAST
Transmembranei536 – 55621Helical; Reviewed prediction
Add
BLAST
Transmembranei571 – 59121Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 908908PTS system glucose-specific EIICBA component
PRO_0000186559Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243273.MG_069.

Structurei

3D structure databases

ProteinModelPortaliP47315.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 264264PTS EIIC type-1; first part
Add
BLAST
Domaini451 – 602152PTS EIIC type-1; second part
Add
BLAST
Domaini631 – 71383PTS EIIB type-1
Add
BLAST
Domaini762 – 875114PTS EIIA type-1
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni265 – 450186Unknown
Add
BLAST

Domaini

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1263.
KOiK02777.
K02778.
K02779.
OMAiFYAPLWY.
OrthoDBiEOG6FFS9V.

Family and domain databases

Gene3Di3.30.1360.60. 1 hit.
InterProiIPR011055. Dup_hybrid_motif.
IPR018113. PTrfase_EIIB_Cys.
IPR001127. PTS_EIIA_1_perm.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
[Graphical view]
PfamiPF00358. PTS_EIIA_1. 1 hit.
PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 2 hits.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
SSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00830. PTBA. 1 hit.
PROSITEiPS51093. PTS_EIIA_TYPE_1. 1 hit.
PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47315-1 [UniParc]FASTAAdd to Basket

« Hide

MQISLVKIRN KFKQRNRGSF RQWVGKLSNG LMIPIAVLPL AGIFLGIGDA    50
ISSNSSGIVG VKFFGEFIKQ GGNVVFANLP ILFAVAIAIT FSQDAGVAGF 100
SAFVFWATMN AFMSSLIIPV DANNTASGYN ILYWKAVPQS AIASTLGLNS 150
LSTSVFGGII VGALTAYLYN KFYAIRLPDV IGFFSGTRFV PIICMTIAIP 200
VALLLLMVWP GVSILLNLIG TGLGILGGRG YGANSLIFGY IERALIPFGV 250
HHAFYAPLWY TSAGGSLQEI ANQQVWIRAP GSDYVTRVIG WEDFNTPGKW 300
VIPAALANGT SGMMNGATTT GQDSTSALSK YMSKESTNFL SWKELVDGLT 350
RKGNFDELAK NGLLDGSNKI WIGLNQSGIL GKKVLLSDGK DYTITFKTFA 400
NTTPTFWSHG AHALLPISGT PSAITNGVTV NGTANSKTYN VSQFTVAVPS 450
LNPAQYSQGK FPFMLIGIPA AGLAMILAAP KGRRKEASSI IGSAAFTSFL 500
TGITEPFEFT FLFLAPWLFY GIHAVLAAVS FWLMNLLSAN VGQTFSGSFI 550
DFILYGALPD GRGWLANSYL VPIIGIFLAL IYFPTFYFLT IRFNLATPGR 600
GGKLITKKEY LAAKAAQKTD QTTNTNFNQT QIEAGMLLRA YGGSENIAEL 650
GACITKLRVT VKNPELVNET IIKDLGAAGV MRTTPTFFVA VFGTRAAVYK 700
SAMQDIIQGK VNWTELQKVL DKNDSTVEKP EIKPTPVLKV QDEIVILSPV 750
NGTLKPLTQV PDDTFKNRLV GDGIAILPSD GHFKAPGDVG VKTELAFPTG 800
HAFIFDVDGV KVMLHIGIDT VKINADKKPG EQLEVFDVKT KQGEYTKLKS 850
ESVVEVDLKK LKRKYDPITP FIVMQESLDN FKLVPIRQRG EIKVGQPLFK 900
LIYKDKKS 908
Length:908
Mass (Da):98,399
Last modified:February 1, 1996 - v1
Checksum:iA079970CF625D9E5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71287.1.
U02207 Genomic DNA. Translation: AAD12499.1.
PIRiF64207.
RefSeqiNP_072731.1. NC_000908.2.
WP_009885925.1. NZ_AAGX01000011.1.

Genome annotation databases

EnsemblBacteriaiAAC71287; AAC71287; MG_069.
GeneIDi875466.
KEGGimge:MG_069.
PATRICi20009540. VBIMycGen98045_0079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71287.1 .
U02207 Genomic DNA. Translation: AAD12499.1 .
PIRi F64207.
RefSeqi NP_072731.1. NC_000908.2.
WP_009885925.1. NZ_AAGX01000011.1.

3D structure databases

ProteinModelPortali P47315.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243273.MG_069.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC71287 ; AAC71287 ; MG_069 .
GeneIDi 875466.
KEGGi mge:MG_069.
PATRICi 20009540. VBIMycGen98045_0079.

Phylogenomic databases

eggNOGi COG1263.
KOi K02777.
K02778.
K02779.
OMAi FYAPLWY.
OrthoDBi EOG6FFS9V.

Enzyme and pathway databases

BioCyci MGEN243273:GH2R-72-MONOMER.

Family and domain databases

Gene3Di 3.30.1360.60. 1 hit.
InterProi IPR011055. Dup_hybrid_motif.
IPR018113. PTrfase_EIIB_Cys.
IPR001127. PTS_EIIA_1_perm.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
[Graphical view ]
Pfami PF00358. PTS_EIIA_1. 1 hit.
PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 2 hits.
[Graphical view ]
SUPFAMi SSF51261. SSF51261. 1 hit.
SSF55604. SSF55604. 1 hit.
TIGRFAMsi TIGR00826. EIIB_glc. 1 hit.
TIGR00830. PTBA. 1 hit.
PROSITEi PS51093. PTS_EIIA_TYPE_1. 1 hit.
PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 284-375.
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  3. "Novel phosphotransferase system genes revealed by bacterial genome analysis: the complete complement of pts genes in Mycoplasma genitalium."
    Reizer J., Paulsen I.T., Reizer A., Titgemeyer F., Saier M.H. Jr.
    Microb. Comp. Genomics 1:151-164(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiPTG3C_MYCGE
AccessioniPrimary (citable) accession number: P47315
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 3, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi