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P47315

- PTG3C_MYCGE

UniProt

P47315 - PTG3C_MYCGE

Protein

PTS system glucose-specific EIICBA component

Gene

ptsG

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport.

    Catalytic activityi

    Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.PROSITE-ProRule annotation
    Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei653 – 6531Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation
    Active sitei815 – 8151Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. kinase activity Source: UniProtKB-KW
    2. protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Phosphotransferase system, Sugar transport, Transport

    Enzyme and pathway databases

    BioCyciMGEN243273:GH2R-72-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    PTS system glucose-specific EIICBA component
    Alternative name(s):
    EII-Glc/EIII-Glc
    EIICBA-Glc
    Including the following 3 domains:
    Glucose permease IIC component
    Alternative name(s):
    PTS system glucose-specific EIIC component
    Glucose-specific phosphotransferase enzyme IIB component (EC:2.7.1.69)
    Alternative name(s):
    PTS system glucose-specific EIIB component
    Glucose-specific phosphotransferase enzyme IIA component (EC:2.7.1.-)
    Alternative name(s):
    PTS system glucose-specific EIIA component
    Gene namesi
    Name:ptsG
    Ordered Locus Names:MG069
    OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
    Taxonomic identifieri243273 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000807: Chromosome

    Subcellular locationi

    Cell membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 908908PTS system glucose-specific EIICBA componentPRO_0000186559Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi243273.MG_069.

    Structurei

    3D structure databases

    ProteinModelPortaliP47315.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei31 – 5121HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei71 – 9121HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei100 – 12021HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei155 – 17521HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei189 – 20921HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei459 – 47921HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei487 – 50721HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei509 – 52921HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei536 – 55621HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei571 – 59121HelicalPROSITE-ProRule annotationAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 264264PTS EIIC type-1; first partPROSITE-ProRule annotationAdd
    BLAST
    Domaini451 – 602152PTS EIIC type-1; second partPROSITE-ProRule annotationAdd
    BLAST
    Domaini631 – 71383PTS EIIB type-1PROSITE-ProRule annotationAdd
    BLAST
    Domaini762 – 875114PTS EIIA type-1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni265 – 450186UnknownAdd
    BLAST

    Domaini

    The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
    The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
    The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

    Sequence similaritiesi

    Contains 1 PTS EIIA type-1 domain.PROSITE-ProRule annotation
    Contains 1 PTS EIIB type-1 domain.PROSITE-ProRule annotation
    Contains 1 PTS EIIC type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1263.
    KOiK02777.
    K02778.
    K02779.
    OMAiFYAPLWY.
    OrthoDBiEOG6FFS9V.

    Family and domain databases

    Gene3Di3.30.1360.60. 1 hit.
    InterProiIPR011055. Dup_hybrid_motif.
    IPR018113. PTrfase_EIIB_Cys.
    IPR001127. PTS_EIIA_1_perm.
    IPR003352. PTS_EIIC.
    IPR013013. PTS_EIIC_1.
    IPR001996. PTS_IIB_1.
    [Graphical view]
    PfamiPF00358. PTS_EIIA_1. 1 hit.
    PF00367. PTS_EIIB. 1 hit.
    PF02378. PTS_EIIC. 2 hits.
    [Graphical view]
    SUPFAMiSSF51261. SSF51261. 1 hit.
    SSF55604. SSF55604. 1 hit.
    TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
    TIGR00830. PTBA. 1 hit.
    PROSITEiPS51093. PTS_EIIA_TYPE_1. 1 hit.
    PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
    PS51098. PTS_EIIB_TYPE_1. 1 hit.
    PS51103. PTS_EIIC_TYPE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47315-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQISLVKIRN KFKQRNRGSF RQWVGKLSNG LMIPIAVLPL AGIFLGIGDA    50
    ISSNSSGIVG VKFFGEFIKQ GGNVVFANLP ILFAVAIAIT FSQDAGVAGF 100
    SAFVFWATMN AFMSSLIIPV DANNTASGYN ILYWKAVPQS AIASTLGLNS 150
    LSTSVFGGII VGALTAYLYN KFYAIRLPDV IGFFSGTRFV PIICMTIAIP 200
    VALLLLMVWP GVSILLNLIG TGLGILGGRG YGANSLIFGY IERALIPFGV 250
    HHAFYAPLWY TSAGGSLQEI ANQQVWIRAP GSDYVTRVIG WEDFNTPGKW 300
    VIPAALANGT SGMMNGATTT GQDSTSALSK YMSKESTNFL SWKELVDGLT 350
    RKGNFDELAK NGLLDGSNKI WIGLNQSGIL GKKVLLSDGK DYTITFKTFA 400
    NTTPTFWSHG AHALLPISGT PSAITNGVTV NGTANSKTYN VSQFTVAVPS 450
    LNPAQYSQGK FPFMLIGIPA AGLAMILAAP KGRRKEASSI IGSAAFTSFL 500
    TGITEPFEFT FLFLAPWLFY GIHAVLAAVS FWLMNLLSAN VGQTFSGSFI 550
    DFILYGALPD GRGWLANSYL VPIIGIFLAL IYFPTFYFLT IRFNLATPGR 600
    GGKLITKKEY LAAKAAQKTD QTTNTNFNQT QIEAGMLLRA YGGSENIAEL 650
    GACITKLRVT VKNPELVNET IIKDLGAAGV MRTTPTFFVA VFGTRAAVYK 700
    SAMQDIIQGK VNWTELQKVL DKNDSTVEKP EIKPTPVLKV QDEIVILSPV 750
    NGTLKPLTQV PDDTFKNRLV GDGIAILPSD GHFKAPGDVG VKTELAFPTG 800
    HAFIFDVDGV KVMLHIGIDT VKINADKKPG EQLEVFDVKT KQGEYTKLKS 850
    ESVVEVDLKK LKRKYDPITP FIVMQESLDN FKLVPIRQRG EIKVGQPLFK 900
    LIYKDKKS 908
    Length:908
    Mass (Da):98,399
    Last modified:February 1, 1996 - v1
    Checksum:iA079970CF625D9E5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71287.1.
    U02207 Genomic DNA. Translation: AAD12499.1.
    PIRiF64207.
    RefSeqiNP_072731.1. NC_000908.2.
    WP_009885925.1. NZ_AAGX01000011.1.

    Genome annotation databases

    EnsemblBacteriaiAAC71287; AAC71287; MG_069.
    GeneIDi875466.
    KEGGimge:MG_069.
    PATRICi20009540. VBIMycGen98045_0079.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71287.1 .
    U02207 Genomic DNA. Translation: AAD12499.1 .
    PIRi F64207.
    RefSeqi NP_072731.1. NC_000908.2.
    WP_009885925.1. NZ_AAGX01000011.1.

    3D structure databases

    ProteinModelPortali P47315.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243273.MG_069.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC71287 ; AAC71287 ; MG_069 .
    GeneIDi 875466.
    KEGGi mge:MG_069.
    PATRICi 20009540. VBIMycGen98045_0079.

    Phylogenomic databases

    eggNOGi COG1263.
    KOi K02777.
    K02778.
    K02779.
    OMAi FYAPLWY.
    OrthoDBi EOG6FFS9V.

    Enzyme and pathway databases

    BioCyci MGEN243273:GH2R-72-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1360.60. 1 hit.
    InterProi IPR011055. Dup_hybrid_motif.
    IPR018113. PTrfase_EIIB_Cys.
    IPR001127. PTS_EIIA_1_perm.
    IPR003352. PTS_EIIC.
    IPR013013. PTS_EIIC_1.
    IPR001996. PTS_IIB_1.
    [Graphical view ]
    Pfami PF00358. PTS_EIIA_1. 1 hit.
    PF00367. PTS_EIIB. 1 hit.
    PF02378. PTS_EIIC. 2 hits.
    [Graphical view ]
    SUPFAMi SSF51261. SSF51261. 1 hit.
    SSF55604. SSF55604. 1 hit.
    TIGRFAMsi TIGR00826. EIIB_glc. 1 hit.
    TIGR00830. PTBA. 1 hit.
    PROSITEi PS51093. PTS_EIIA_TYPE_1. 1 hit.
    PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
    PS51098. PTS_EIIB_TYPE_1. 1 hit.
    PS51103. PTS_EIIC_TYPE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33530 / G-37 / NCTC 10195.
    2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
      Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
      J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 284-375.
      Strain: ATCC 33530 / G-37 / NCTC 10195.
    3. "Novel phosphotransferase system genes revealed by bacterial genome analysis: the complete complement of pts genes in Mycoplasma genitalium."
      Reizer J., Paulsen I.T., Reizer A., Titgemeyer F., Saier M.H. Jr.
      Microb. Comp. Genomics 1:151-164(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISCUSSION OF SEQUENCE.

    Entry informationi

    Entry nameiPTG3C_MYCGE
    AccessioniPrimary (citable) accession number: P47315
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Mycoplasma genitalium
      Mycoplasma genitalium (strain G-37): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3