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Protein

PTS system glucose-specific EIICBA component

Gene

ptsG

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport.

Catalytic activityi

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.PROSITE-ProRule annotation
Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei653 – 6531Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation
Active sitei815 – 8151Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-72-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system glucose-specific EIICBA component
Alternative name(s):
EII-Glc/EIII-Glc
EIICBA-Glc
Including the following 3 domains:
Glucose permease IIC component
Alternative name(s):
PTS system glucose-specific EIIC component
Glucose-specific phosphotransferase enzyme IIB component (EC:2.7.1.69)
Alternative name(s):
PTS system glucose-specific EIIB component
Glucose-specific phosphotransferase enzyme IIA component (EC:2.7.1.-)
Alternative name(s):
PTS system glucose-specific EIIA component
Gene namesi
Name:ptsG
Ordered Locus Names:MG069
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807 Componenti: Chromosome

Subcellular locationi

  • Cell membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei31 – 5121HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei71 – 9121HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei100 – 12021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei155 – 17521HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei189 – 20921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei459 – 47921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei487 – 50721HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei509 – 52921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei536 – 55621HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei571 – 59121HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 908908PTS system glucose-specific EIICBA componentPRO_0000186559Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243273.MgenG_010200002836.

Structurei

3D structure databases

ProteinModelPortaliP47315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 264264PTS EIIC type-1; first partPROSITE-ProRule annotationAdd
BLAST
Domaini451 – 602152PTS EIIC type-1; second partPROSITE-ProRule annotationAdd
BLAST
Domaini631 – 71383PTS EIIB type-1PROSITE-ProRule annotationAdd
BLAST
Domaini762 – 875114PTS EIIA type-1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni265 – 450186UnknownAdd
BLAST

Domaini

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Sequence similaritiesi

Contains 1 PTS EIIA type-1 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIB type-1 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1263.
KOiK02777.
K02778.
K02779.
OMAiFYAPLWY.
OrthoDBiEOG6FFS9V.

Family and domain databases

Gene3Di3.30.1360.60. 1 hit.
InterProiIPR011055. Dup_hybrid_motif.
IPR018113. PTrfase_EIIB_Cys.
IPR001127. PTS_EIIA_1_perm.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
[Graphical view]
PfamiPF00358. PTS_EIIA_1. 1 hit.
PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 2 hits.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
SSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00830. PTBA. 1 hit.
PROSITEiPS51093. PTS_EIIA_TYPE_1. 1 hit.
PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47315-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQISLVKIRN KFKQRNRGSF RQWVGKLSNG LMIPIAVLPL AGIFLGIGDA
60 70 80 90 100
ISSNSSGIVG VKFFGEFIKQ GGNVVFANLP ILFAVAIAIT FSQDAGVAGF
110 120 130 140 150
SAFVFWATMN AFMSSLIIPV DANNTASGYN ILYWKAVPQS AIASTLGLNS
160 170 180 190 200
LSTSVFGGII VGALTAYLYN KFYAIRLPDV IGFFSGTRFV PIICMTIAIP
210 220 230 240 250
VALLLLMVWP GVSILLNLIG TGLGILGGRG YGANSLIFGY IERALIPFGV
260 270 280 290 300
HHAFYAPLWY TSAGGSLQEI ANQQVWIRAP GSDYVTRVIG WEDFNTPGKW
310 320 330 340 350
VIPAALANGT SGMMNGATTT GQDSTSALSK YMSKESTNFL SWKELVDGLT
360 370 380 390 400
RKGNFDELAK NGLLDGSNKI WIGLNQSGIL GKKVLLSDGK DYTITFKTFA
410 420 430 440 450
NTTPTFWSHG AHALLPISGT PSAITNGVTV NGTANSKTYN VSQFTVAVPS
460 470 480 490 500
LNPAQYSQGK FPFMLIGIPA AGLAMILAAP KGRRKEASSI IGSAAFTSFL
510 520 530 540 550
TGITEPFEFT FLFLAPWLFY GIHAVLAAVS FWLMNLLSAN VGQTFSGSFI
560 570 580 590 600
DFILYGALPD GRGWLANSYL VPIIGIFLAL IYFPTFYFLT IRFNLATPGR
610 620 630 640 650
GGKLITKKEY LAAKAAQKTD QTTNTNFNQT QIEAGMLLRA YGGSENIAEL
660 670 680 690 700
GACITKLRVT VKNPELVNET IIKDLGAAGV MRTTPTFFVA VFGTRAAVYK
710 720 730 740 750
SAMQDIIQGK VNWTELQKVL DKNDSTVEKP EIKPTPVLKV QDEIVILSPV
760 770 780 790 800
NGTLKPLTQV PDDTFKNRLV GDGIAILPSD GHFKAPGDVG VKTELAFPTG
810 820 830 840 850
HAFIFDVDGV KVMLHIGIDT VKINADKKPG EQLEVFDVKT KQGEYTKLKS
860 870 880 890 900
ESVVEVDLKK LKRKYDPITP FIVMQESLDN FKLVPIRQRG EIKVGQPLFK

LIYKDKKS
Length:908
Mass (Da):98,399
Last modified:February 1, 1996 - v1
Checksum:iA079970CF625D9E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71287.1.
U02207 Genomic DNA. Translation: AAD12499.1.
PIRiF64207.
RefSeqiNP_072731.1. NC_000908.2.
WP_009885925.1. NZ_AAGX01000011.1.

Genome annotation databases

EnsemblBacteriaiAAC71287; AAC71287; MG_069.
KEGGimge:MG_069.
PATRICi20009540. VBIMycGen98045_0079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71287.1.
U02207 Genomic DNA. Translation: AAD12499.1.
PIRiF64207.
RefSeqiNP_072731.1. NC_000908.2.
WP_009885925.1. NZ_AAGX01000011.1.

3D structure databases

ProteinModelPortaliP47315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243273.MgenG_010200002836.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71287; AAC71287; MG_069.
KEGGimge:MG_069.
PATRICi20009540. VBIMycGen98045_0079.

Phylogenomic databases

eggNOGiCOG1263.
KOiK02777.
K02778.
K02779.
OMAiFYAPLWY.
OrthoDBiEOG6FFS9V.

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-72-MONOMER.

Family and domain databases

Gene3Di3.30.1360.60. 1 hit.
InterProiIPR011055. Dup_hybrid_motif.
IPR018113. PTrfase_EIIB_Cys.
IPR001127. PTS_EIIA_1_perm.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
[Graphical view]
PfamiPF00358. PTS_EIIA_1. 1 hit.
PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 2 hits.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
SSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00830. PTBA. 1 hit.
PROSITEiPS51093. PTS_EIIA_TYPE_1. 1 hit.
PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 284-375.
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  3. "Novel phosphotransferase system genes revealed by bacterial genome analysis: the complete complement of pts genes in Mycoplasma genitalium."
    Reizer J., Paulsen I.T., Reizer A., Titgemeyer F., Saier M.H. Jr.
    Microb. Comp. Genomics 1:151-164(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiPTG3C_MYCGE
AccessioniPrimary (citable) accession number: P47315
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.