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P47315 (PTG3C_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PTS system glucose-specific EIICBA component
Alternative name(s):
EII-Glc/EIII-Glc
EIICBA-Glc

Including the following 3 domains:

  1. Glucose permease IIC component
    Alternative name(s):
    PTS system glucose-specific EIIC component
  2. Glucose-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
    Alternative name(s):
    PTS system glucose-specific EIIB component
  3. Glucose-specific phosphotransferase enzyme IIA component
    EC=2.7.1.-
    Alternative name(s):
    PTS system glucose-specific EIIA component
Gene names
Name:ptsG
Ordered Locus Names:MG069
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length908 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport.

Catalytic activity

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Domain

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Sequence similarities

Contains 1 PTS EIIA type-1 domain.

Contains 1 PTS EIIB type-1 domain.

Contains 1 PTS EIIC type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 908908PTS system glucose-specific EIICBA component
PRO_0000186559

Regions

Transmembrane31 – 5121Helical; Potential
Transmembrane71 – 9121Helical; Potential
Transmembrane100 – 12021Helical; Potential
Transmembrane155 – 17521Helical; Potential
Transmembrane189 – 20921Helical; Potential
Transmembrane459 – 47921Helical; Potential
Transmembrane487 – 50721Helical; Potential
Transmembrane509 – 52921Helical; Potential
Transmembrane536 – 55621Helical; Potential
Transmembrane571 – 59121Helical; Potential
Domain1 – 264264PTS EIIC type-1; first part
Domain451 – 602152PTS EIIC type-1; second part
Domain631 – 71383PTS EIIB type-1
Domain762 – 875114PTS EIIA type-1
Region265 – 450186Unknown

Sites

Active site6531Phosphocysteine intermediate; for EIIB activity By similarity
Active site8151Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P47315 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: A079970CF625D9E5

FASTA90898,399
        10         20         30         40         50         60 
MQISLVKIRN KFKQRNRGSF RQWVGKLSNG LMIPIAVLPL AGIFLGIGDA ISSNSSGIVG 

        70         80         90        100        110        120 
VKFFGEFIKQ GGNVVFANLP ILFAVAIAIT FSQDAGVAGF SAFVFWATMN AFMSSLIIPV 

       130        140        150        160        170        180 
DANNTASGYN ILYWKAVPQS AIASTLGLNS LSTSVFGGII VGALTAYLYN KFYAIRLPDV 

       190        200        210        220        230        240 
IGFFSGTRFV PIICMTIAIP VALLLLMVWP GVSILLNLIG TGLGILGGRG YGANSLIFGY 

       250        260        270        280        290        300 
IERALIPFGV HHAFYAPLWY TSAGGSLQEI ANQQVWIRAP GSDYVTRVIG WEDFNTPGKW 

       310        320        330        340        350        360 
VIPAALANGT SGMMNGATTT GQDSTSALSK YMSKESTNFL SWKELVDGLT RKGNFDELAK 

       370        380        390        400        410        420 
NGLLDGSNKI WIGLNQSGIL GKKVLLSDGK DYTITFKTFA NTTPTFWSHG AHALLPISGT 

       430        440        450        460        470        480 
PSAITNGVTV NGTANSKTYN VSQFTVAVPS LNPAQYSQGK FPFMLIGIPA AGLAMILAAP 

       490        500        510        520        530        540 
KGRRKEASSI IGSAAFTSFL TGITEPFEFT FLFLAPWLFY GIHAVLAAVS FWLMNLLSAN 

       550        560        570        580        590        600 
VGQTFSGSFI DFILYGALPD GRGWLANSYL VPIIGIFLAL IYFPTFYFLT IRFNLATPGR 

       610        620        630        640        650        660 
GGKLITKKEY LAAKAAQKTD QTTNTNFNQT QIEAGMLLRA YGGSENIAEL GACITKLRVT 

       670        680        690        700        710        720 
VKNPELVNET IIKDLGAAGV MRTTPTFFVA VFGTRAAVYK SAMQDIIQGK VNWTELQKVL 

       730        740        750        760        770        780 
DKNDSTVEKP EIKPTPVLKV QDEIVILSPV NGTLKPLTQV PDDTFKNRLV GDGIAILPSD 

       790        800        810        820        830        840 
GHFKAPGDVG VKTELAFPTG HAFIFDVDGV KVMLHIGIDT VKINADKKPG EQLEVFDVKT 

       850        860        870        880        890        900 
KQGEYTKLKS ESVVEVDLKK LKRKYDPITP FIVMQESLDN FKLVPIRQRG EIKVGQPLFK 


LIYKDKKS 

« Hide

References

« Hide 'large scale' references
[1]"The minimal gene complement of Mycoplasma genitalium."
Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. expand/collapse author list , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.
Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]"A survey of the Mycoplasma genitalium genome by using random sequencing."
Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 284-375.
Strain: ATCC 33530 / G-37 / NCTC 10195.
[3]"Novel phosphotransferase system genes revealed by bacterial genome analysis: the complete complement of pts genes in Mycoplasma genitalium."
Reizer J., Paulsen I.T., Reizer A., Titgemeyer F., Saier M.H. Jr.
Microb. Comp. Genomics 1:151-164(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC71287.1.
U02207 Genomic DNA. Translation: AAD12499.1.
PIRF64207.
RefSeqNP_072731.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47315.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_069.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC71287; AAC71287; MG_069.
GeneID875466.
KEGGmge:MG_069.
PATRIC20009540. VBIMycGen98045_0079.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1263.
KOK02777.
K02778.
K02779.
OMAFYAPLWY.
OrthoDBEOG6FFS9V.
ProtClustDBCLSK542132.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-72-MONOMER.

Family and domain databases

Gene3D3.30.1360.60. 1 hit.
InterProIPR011055. Dup_hybrid_motif.
IPR018113. PTrfase_EIIB/Cys_phosph_CS.
IPR001127. PTS_EIIA_1_perm.
IPR001996. PTS_EIIB_1.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR011535. PTS_Glc-like_IIB_component.
[Graphical view]
PfamPF00358. PTS_EIIA_1. 1 hit.
PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 2 hits.
[Graphical view]
SUPFAMSSF51261. SSF51261. 1 hit.
SSF55604. SSF55604. 1 hit.
TIGRFAMsTIGR00826. EIIB_glc. 1 hit.
TIGR00830. PTBA. 1 hit.
PROSITEPS51093. PTS_EIIA_TYPE_1. 1 hit.
PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePTG3C_MYCGE
AccessionPrimary (citable) accession number: P47315
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names