P47315 (PTG3C_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PTS system glucose-specific EIICBA component

Alternative name(s):
EII-Glc/EIII-Glc
EIICBA-Glc

Including the following 3 domains:

1. Glucose permease IIC component
   Alternative name(s):
   PTS system glucose-specific EIIC component
2. Glucose-specific phosphotransferase enzyme IIB component
   EC=2.7.1.69
   Alternative name(s):
   PTS system glucose-specific EIIB component
3. Glucose-specific phosphotransferase enzyme IIA component
   EC=2.7.1.-
   Alternative name(s):
   PTS system glucose-specific EIIA component

Gene names

Name:	ptsG
Ordered Locus Names:	MG069

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	908 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-1 domain. Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 908	908	PTS system glucose-specific EIICBA component		PRO_0000186559
Regions
	Transmembrane	31 – 51	21	Helical; Potential
	Transmembrane	71 – 91	21	Helical; Potential
	Transmembrane	100 – 120	21	Helical; Potential
	Transmembrane	155 – 175	21	Helical; Potential
	Transmembrane	189 – 209	21	Helical; Potential
	Transmembrane	459 – 479	21	Helical; Potential
	Transmembrane	487 – 507	21	Helical; Potential
	Transmembrane	509 – 529	21	Helical; Potential
	Transmembrane	536 – 556	21	Helical; Potential
	Transmembrane	571 – 591	21	Helical; Potential
	Domain	1 – 264	264	PTS EIIC type-1; first part
	Domain	451 – 602	152	PTS EIIC type-1; second part
	Domain	631 – 713	83	PTS EIIB type-1
	Domain	762 – 875	114	PTS EIIA type-1
	Region	265 – 450	186	Unknown
Sites
	Active site	653	1	Phosphocysteine intermediate; for EIIB activity By similarity
	Active site	815	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47315 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: A079970CF625D9E5	FASTA	908	98,399
	10 20 30 40 50 60 MQISLVKIRN KFKQRNRGSF RQWVGKLSNG LMIPIAVLPL AGIFLGIGDA ISSNSSGIVG 70 80 90 100 110 120 VKFFGEFIKQ GGNVVFANLP ILFAVAIAIT FSQDAGVAGF SAFVFWATMN AFMSSLIIPV 130 140 150 160 170 180 DANNTASGYN ILYWKAVPQS AIASTLGLNS LSTSVFGGII VGALTAYLYN KFYAIRLPDV 190 200 210 220 230 240 IGFFSGTRFV PIICMTIAIP VALLLLMVWP GVSILLNLIG TGLGILGGRG YGANSLIFGY 250 260 270 280 290 300 IERALIPFGV HHAFYAPLWY TSAGGSLQEI ANQQVWIRAP GSDYVTRVIG WEDFNTPGKW 310 320 330 340 350 360 VIPAALANGT SGMMNGATTT GQDSTSALSK YMSKESTNFL SWKELVDGLT RKGNFDELAK 370 380 390 400 410 420 NGLLDGSNKI WIGLNQSGIL GKKVLLSDGK DYTITFKTFA NTTPTFWSHG AHALLPISGT 430 440 450 460 470 480 PSAITNGVTV NGTANSKTYN VSQFTVAVPS LNPAQYSQGK FPFMLIGIPA AGLAMILAAP 490 500 510 520 530 540 KGRRKEASSI IGSAAFTSFL TGITEPFEFT FLFLAPWLFY GIHAVLAAVS FWLMNLLSAN 550 560 570 580 590 600 VGQTFSGSFI DFILYGALPD GRGWLANSYL VPIIGIFLAL IYFPTFYFLT IRFNLATPGR 610 620 630 640 650 660 GGKLITKKEY LAAKAAQKTD QTTNTNFNQT QIEAGMLLRA YGGSENIAEL GACITKLRVT 670 680 690 700 710 720 VKNPELVNET IIKDLGAAGV MRTTPTFFVA VFGTRAAVYK SAMQDIIQGK VNWTELQKVL 730 740 750 760 770 780 DKNDSTVEKP EIKPTPVLKV QDEIVILSPV NGTLKPLTQV PDDTFKNRLV GDGIAILPSD 790 800 810 820 830 840 GHFKAPGDVG VKTELAFPTG HAFIFDVDGV KVMLHIGIDT VKINADKKPG EQLEVFDVKT 850 860 870 880 890 900 KQGEYTKLKS ESVVEVDLKK LKRKYDPITP FIVMQESLDN FKLVPIRQRG EIKVGQPLFK LIYKDKKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 284-375. Strain: ATCC 33530 / G-37 / NCTC 10195.
[3]	"Novel phosphotransferase system genes revealed by bacterial genome analysis: the complete complement of pts genes in Mycoplasma genitalium." Reizer J., Paulsen I.T., Reizer A., Titgemeyer F., Saier M.H. Jr. Microb. Comp. Genomics 1:151-164(1996) [PubMed] [Europe PMC] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71287.1. U02207 Genomic DNA. Translation: AAD12499.1.
PIR	F64207.
RefSeq	NP_072731.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47315.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_069.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71287; AAC71287; MG_069.
GeneID	875466.
KEGG	mge:MG_069.
PATRIC	20009540. VBIMycGen98045_0079.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1263.
KO	K02777. K02778. K02779.
OMA	FLFLAPW.
ProtClustDB	CLSK542132.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-72-MONOMER.
Family and domain databases
Gene3D	3.30.1360.60. 1 hit.
InterPro	IPR011055. Dup_hybrid_motif. IPR018113. PTrfase_EIIB/Cys_phosph_CS. IPR001127. PTS_EIIA_1_perm. IPR001996. PTS_EIIB_1. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. IPR011535. PTS_Glc-like_IIB_component. [Graphical view]
Pfam	PF00358. PTS_EIIA_1. 1 hit. PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 2 hits. [Graphical view]
SUPFAM	SSF51261. Dup_hybrid_motif. 1 hit. SSF55604. PTS_EIIB. 1 hit.
TIGRFAMs	TIGR00826. EIIB_glc. 1 hit. TIGR00830. PTBA. 1 hit.
PROSITE	PS51093. PTS_EIIA_TYPE_1. 1 hit. PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit. PS51098. PTS_EIIB_TYPE_1. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTG3C_MYCGE

Accession

Primary (citable) accession number: P47315

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program