P47312 (TKT_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transketolase
Short name=TK
EC=2.2.1.1

Gene names

Name:	tkt
Synonyms:	tktA
Ordered Locus Names:	MG066

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate.
Cofactor	Binds 1 calcium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Sequence similarities	Belongs to the transketolase family.

Ontologies

Keywords
Ligand	Calcium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW transketolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 648	648	Transketolase		PRO_0000191859
Sites
	Metal binding	150	1	Calcium By similarity
	Metal binding	180	1	Calcium By similarity
	Metal binding	182	1	Calcium; via carbonyl oxygen By similarity
Experimental info
	Sequence conflict	24	1	G → C in AAD12435. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47312 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 0252E8DDD2BFB453	FASTA	648	73,785
	10 20 30 40 50 60 MKYLYATQHL TLNAIKHAKG GHVGMAIGAS PILFSLFTKH FHFDPDQPKW INRDRFVLSA 70 80 90 100 110 120 GHGSMALYSI FHFAGLISKQ EILQHKHGQI NTSSHPEYAP NNFIDASTGP LGQGFGMAVG 130 140 150 160 170 180 MVLAQKLLAN EFKELSDKLF DHYTYVVVGD GDLQEGVSYE VSQIAGLYKL NKLIVLHDSN 190 200 210 220 230 240 RVQMDSEVKK VANENLKVRF ENVGWNYIHT DDQLENIDQA IIKAKQSDKP TFIEVRTTIA 250 260 270 280 290 300 KNTHLEDQYG GHWFIPNEVD FQLFEKRTNT NFNFFNYPDS IYHWFKQTVI ERQKQIKEDY 310 320 330 340 350 360 NNLLISLKDK PLFKKFTNWI DSDFQALYLN QLDEKKVAKK DSATRNYLKD FLNQINNPNS 370 380 390 400 410 420 NLYCLNADVS RSCFIKIGDD NLHENPCSRN IQIGIREFAM ATIMNGMALH GGIKVMGGTF 430 440 450 460 470 480 LAFADYSKPA IRLGALMNLP VFYVYTHDSY QVGGDGPTHQ PYDQLPMLRA IENVCVFRPC 490 500 510 520 530 540 DEKETCAGFN YGLLSQDQTT VLVLTRQPLK SIDNTDSLKT LKGGYILLDR KQPDLIIAAS 550 560 570 580 590 600 GSEVQLAIEF EKVLTKQNVK VRILSVPNIT LLLKQDEKYL KSLFDANSSL ITIEASSSYE 610 620 630 640 WFCFKKYVKN HAHLGAFSFG ESDDGDKVYQ QKGFNLERLM KIFTSLRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42. Strain: ATCC 33530 / G-37 / NCTC 10195.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71284.1. U02154 Genomic DNA. Translation: AAD12435.1.
PIR	C64207.
RefSeq	NP_072728.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47312.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	875474.
GenomeReviews	Gene locus MG066 in contig L43967_GR.
KEGG	mge:MG_066.
PATRIC	20009518. VBIMycGen98045_0068.
TIGR	MG066.
Phylogenomic databases
HOGENOM	HBG565885.
OMA	LASIPCL.
PhylomeDB	P47312.
ProtClustDB	PRK05899.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_066-MONOMER.
Family and domain databases
InterPro	IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005478. Transketolase_bac-like. IPR020826. Transketolase_BS. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K00615.
Pfam	PF02779. Transket_pyr. 1 hit. PF00456. Transketolase_N. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00232. Tktlase_bact. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. False negative. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TKT_MYCGE

Accession

Primary (citable) accession number: P47312
Secondary accession number(s): Q49280

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program