P47308 (PTF3A_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PTS system fructose-specific EIIABC component

Alternative name(s):
EIIABC-Fru

Including the following 3 domains:

1. Fructose-specific phosphotransferase enzyme IIA component
   EC=2.7.1.-
   Alternative name(s):
   EII-Fru
   PTS system fructose-specific EIIA component
2. Fructose-specific phosphotransferase enzyme IIB component
   EC=2.7.1.69
   Alternative name(s):
   EIII-Fru
   PTS system fructose-specific EIIB component
3. Fructose permease IIC component
   Alternative name(s):
   PTS system fructose-specific EIIC component

Gene names

Name:	fruA
Ordered Locus Names:	MG062

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	680 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Domain	The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
Sequence similarities	Contains 1 PTS EIIA type-2 domain. Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 680	680	PTS system fructose-specific EIIABC component		PRO_0000186510
Regions
	Transmembrane	306 – 326	21	Helical; Potential
	Transmembrane	352 – 372	21	Helical; Potential
	Transmembrane	377 – 397	21	Helical; Potential
	Transmembrane	410 – 430	21	Helical; Potential
	Transmembrane	449 – 469	21	Helical; Potential
	Transmembrane	488 – 508	21	Helical; Potential
	Transmembrane	530 – 550	21	Helical; Potential
	Transmembrane	565 – 585	21	Helical; Potential
	Transmembrane	590 – 610	21	Helical; Potential
	Transmembrane	643 – 663	21	Helical; Potential
	Domain	4 – 149	146	PTS EIIA type-2
	Domain	168 – 264	97	PTS EIIB type-2
	Domain	298 – 675	378	PTS EIIC type-2
Sites
	Active site	68	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity
	Active site	174	1	Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47308 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: A6AC1B1D7AAA9DA4	FASTA	680	74,090
	10 20 30 40 50 60 MFKNLLRPSL FFNWSQKTFK NKFSFLKQAA NALQKQAVIN DNNVAFEALK KREEEITTGI 70 80 90 100 110 120 ITSLALPHLQ SQSVIEPFVA VFKVKNLDWQ SLDQKPVKLI FLIGVLVDKT NLHLDFISNF 130 140 150 160 170 180 SKLMLNETFA SKVLNVTSYN GLIKLIDLFN QQKVQDQPAV ETKKEYDFVA VTACPTGIAH 190 200 210 220 230 240 TFMAKEALEA FAKKHNLYVK VETQGTDGIQ NQLTSDDINN AKGVILACDR LIDFSRFYAN 250 260 270 280 290 300 KNVIEVSTTK AIKKPDEVYE LIKNQKGKQL ANSAKPTNQT QLAESEGEFN FNNFHKRIYR 310 320 330 340 350 360 AILSGVSYML PFVVFGGILI ALSFLIDINN ANNAGGNFGT INPVANWLNK LGGISFSLIV 370 380 390 400 410 420 PILSAYIAYA LVSRQGLLPG FVVGLISSGQ FLLNIVLTNG TIEWLAPSQV SSGFFGAIFG 430 440 450 460 470 480 GLLSACLIIV QQNYIYKKLP QSLQGIKNIL FIPLFGTLFT AGLFWVINIP LIYLNYGLSL 490 500 510 520 530 540 FLNIMNSPIL APLLGFVIGL MMCFDLGGPI NKAAYVFGVV SLQNQNAGTI SMAAAMLSGM 550 560 570 580 590 600 VPPLSIALAA SIRKSCFDKQ ELPAAYACYL MGLSFISEGA IPFVVKKPKV MLTANLIAGA 610 620 630 640 650 660 ICGALTGAFA LSIRAPHGGV FVFALLKTTL QGIEGATLQT GVGIGLALVC LIISMIVGSS 670 680 IIIGYDLIAK HNQRKQNLNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 147-253. Strain: ATCC 33530 / G-37 / NCTC 10195.
[3]	"Novel phosphotransferase system genes revealed by bacterial genome analysis: the complete complement of pts genes in Mycoplasma genitalium." Reizer J., Paulsen I.T., Reizer A., Titgemeyer F., Saier M.H. Jr. Microb. Comp. Genomics 1:151-164(1996) [PubMed: 9689210] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71279.1. U02138 Genomic DNA. Translation: AAD12415.1.
PIR	H64206.
RefSeq	NP_072724.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47308.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	875519.
GenomeReviews	Gene locus MG062 in contig L43967_GR.
KEGG	mge:MG_062.
PATRIC	20009508. VBIMycGen98045_0063.
TIGR	MG062.
Phylogenomic databases
HOGENOM	HBG704192.
OMA	ISFLIPE.
PhylomeDB	P47308.
ProtClustDB	CLSK542128.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_062-MONOMER.
Family and domain databases
InterPro	IPR016152. PTrfase/Anion_transptr. IPR002178. PTS_EIIA_2. IPR013011. PTS_EIIB_2. IPR003501. PTS_EIIB_2/3. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. IPR004715. PTS_IIA_fruc. IPR003353. PTS_IIB_fruc. IPR006327. PTS_IIC_fruc. [Graphical view]
Gene3D	G3DSA:3.40.930.10. PTS_EIIA_2. 1 hit.
KO	K02768. K02769. K02770.
Pfam	PF00359. PTS_EIIA_2. 1 hit. PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
SUPFAM	SSF55804. PTrfase/Anion_transptr. 1 hit.
TIGRFAMs	TIGR00829. FRU. 1 hit. TIGR00848. FruA. 1 hit. TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITE	PS51094. PTS_EIIA_TYPE_2. 1 hit. PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTF3A_MYCGE

Accession

Primary (citable) accession number: P47308

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program