Reviewed, UniProtKB/Swiss-Prot P47304 (KPRS_MYCGE)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase
      Short name=RPPK
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase
      Short name=P-Rib-PP synthetase
      Short name=PRPP synthetase

Gene names

Name:	prs
Synonyms:	prsA
Ordered Locus Names:	MG058

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Ribose-phosphate pyrophosphokinase HAMAP MF_00583		PRO_0000141160
Regions
	Region	230 – 243	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	146	1	Magnesium Potential
	Metal binding	148	1	Magnesium Potential
	Metal binding	157	1	Magnesium Potential
	Metal binding	161	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	P47304-1 [UniParc]. Last modified October 25, 2002. Version 2. Checksum: 41AFB21B36605DAD	FASTA	344	38,881
	10 20 30 40 50 60 MKLLKRKQSD ASLKLSIDNK KHVIFSLSKS KTLVENICKK LNISEGKMVC EHFADGETYI 70 80 90 100 110 120 RFDESVRNKD IYIFQSTCPN VNDSLMELLI AIDALKRGSA KSITAILPYY GYARQDRKTK 130 140 150 160 170 180 GREPITSKLI ADMLTKAGAN RVVLTDIHSD QTQGFFDIPV DSLRTYHIFL FRVIELLGKK 190 200 210 220 230 240 DLVVVSPDYG GVKRARLIAN TLELPLAIID KRRPSHNVAE SINVLGEVKN KNCLIVDDMI 250 260 270 280 290 300 DTGGTVIAAA KLLQKEQAKK VCVMATHGLF NNDAEQKFME AFDQKLIDFL FVSNSIPQYK 310 320 330 340 FKAVKQFEVV DLASLYEEVV LCYANSLSVS AIYERHIEWI KKHV

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43967 Genomic DNA. Translation: AAC71275.1. Different initiation.
PIR	D64206.
RefSeq	NP_072720.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	875549.
GenomeReviews	Gene locus MG058 in contig L43967_GR.
KEGG	mge:MG_058.
TIGR	MG058.
Phylogenomic databases
HOGENOM	P47304.
OMA	CATHAVF
Enzyme and pathway databases
BioCyc	MGEN243273:MG_058-MON.
BRENDA	2.7.6.1. 110.
Family and domain databases
HAMAP	MF_00583. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_MYCGE

Accession

Primary (citable) accession number: P47304

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	October 25, 2002
Last modified:	November 24, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)