P47303 (RNM5_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease M5
EC=3.1.26.8

Alternative name(s):
RNase M5
Ribosomal RNA terminal maturase M5

Gene names

Name:	rnmV
Ordered Locus Names:	MG057

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for correct processing of both the 5' and 3' ends of 5S rRNA precursor. Cleaves both sides of a double-stranded region yielding mature 5S rRNA in one step By similarity. HAMAP-Rule MF_01469
Catalytic activity	Endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor. HAMAP-Rule MF_01469
Cofactor	Magnesium. Binds two Mg2+ per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribonuclease M5 family. Contains 1 Toprim domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding RNA-binding rRNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease M5 activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Ribonuclease M5 HAMAP-Rule MF_01469		PRO_0000210401
Regions
	Domain	10 – 94	85	Toprim
Sites
	Metal binding	16	1	Magnesium 1; catalytic By similarity
	Metal binding	62	1	Magnesium 1; catalytic By similarity
	Metal binding	62	1	Magnesium 2 By similarity
	Metal binding	64	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47303 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 49C5D330D420E64A	FASTA	178	20,497
	10 20 30 40 50 60 MDQKARIKID GVIVCEGKTD QAKLQQIFDV DVITTNGSAL KKETINLIKK ISEKQTVILL 70 80 90 100 110 120 LDPDQQGKKI RSKLEQHLTN YYNCYVDMNA RLKNAKKAGI AEMETSALIA ALNNRVAITK 130 140 150 160 170 NANQSILWDQ YLSLKLNDKK KRLLLCNNLN LPYFNHKQLF KKLNLLNLTF QDVWKHLK

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71274.1.
PIR	C64206.
RefSeq	NP_072719.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47303.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_057.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71274; AAC71274; MG_057.
GeneID	875232.
KEGG	mge:MG_057.
PATRIC	20009496. VBIMycGen98045_0058.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1658.
KO	K05985.
OMA	CETIETN.
ProtClustDB	CLSK542126.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-98-MONOMER.
Family and domain databases
HAMAP	MF_01469. RNase_M5.
InterPro	IPR025156. DUF4093. IPR004466. RNase_M5. IPR006171. Toprim_domain. [Graphical view]
Pfam	PF13331. DUF4093. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
SMART	SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR00334. 5S_RNA_mat_M5. 1 hit.
PROSITE	PS50880. TOPRIM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNM5_MYCGE

Accession

Primary (citable) accession number: P47303

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program