Reviewed, UniProtKB/Swiss-Prot P47294 (SRP54_MYCGE)

Names and origin

Protein names

Recommended name:
    Signal recognition particle protein
Alternative name(s):
    Fifty-four homolog

Gene names

Name:	ffh
Ordered Locus Names:	MG048

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Necessary for efficient export of extracytoplasmic proteins. Binds to the signal sequence when it emerges from the ribosomes By similarity.
Subunit structure	Signal recognition particle consists of a small cytoplasmic RNA (sc-RNA) molecule and protein ffh By similarity.
Domain	The protein has a two domain structure: the G-domain binds GTP; the M-domain binds the RNA and also binds the signal sequence.
Sequence similarities	Belongs to the GTP-binding SRP family.

Ontologies

Keywords
Cellular component	Signal recognition particle
Ligand	GTP-binding Nucleotide-binding RNA-binding
Molecular function	Ribonucleoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	SRP-dependent cotranslational protein targeting to membrane Inferred from electronic annotation. Source: InterPro
Cellular component	signal recognition particle, endoplasmic reticulum targeting Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	7S RNA binding Inferred from electronic annotation. Source: InterPro GTP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Signal recognition particle protein		PRO_0000101159
Regions
	Nucleotide binding	106 – 113	8	GTP By similarity
	Nucleotide binding	188 – 192	5	GTP By similarity
	Nucleotide binding	246 – 249	4	GTP By similarity
	Region	1 – 293	293	G-domain
	Region	294 – 446	153	M-domain

Sequences

	Sequence		Length	Mass (Da)
	P47294-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 080F2255C8B5E5AE	FASTA	446	50,199
	10 20 30 40 50 60 MFKAMLSSIV MRTMQKKINA QTITEKDVEL VLKEIRIALL DADVNLLVVK NFIKAIRDKT 70 80 90 100 110 120 VGQTIEPGQD LQKSLLKTIK TELINILSQP NQELNEKRPL KIMMVGLQGS GKTTTCGKLA 130 140 150 160 170 180 YWLEKKYKQK TMLVGLDIYR PAAIEQLETL SQQTNSVFFA QGTQPVAKTT KAALSAFKTA 190 200 210 220 230 240 KCQTIICDTA GRLQTNETLM DELVSVKNEL NPDEIIMVVD GLSGQEIINV AQTFHKRLKL 250 260 270 280 290 300 TGFIISKLDS DARAGAALSL ASLLQVPIKL IGVSEKLDGL EQFHPERIAN RILGLGDVMS 310 320 330 340 350 360 LVEKAEQVFD KKDLTKTISK MFLGKMDLED LLIYMQQMHK MGSVSSLIKM LPANFSVSEE 370 380 390 400 410 420 NAELIENKIE LWKVLINSMT REERRHPKLI NRDPNRKQRI IKGSGRKMDE LNKLMKEWNK 430 440 MQLKATEMGK LLKTGSNPFG GFGQFF

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43967 Genomic DNA. Translation: AAC71264.1.
PIR	C64205.
RefSeq	NP_072708.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	875253.
GenomeReviews	Gene locus MG048 in contig L43967_GR.
KEGG	mge:MG_048.
TIGR	MG048.
Phylogenomic databases
HOGENOM	P47294.
OMA	IINGSRR
Enzyme and pathway databases
BioCyc	MGEN243273:MG_048-MON.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR000897. Signal_recog_part_SRP54_GTPase. IPR013822. Signal_recog_particl_SRP54_hlx. IPR004780. Signal_recog_particle_SRP. IPR004125. Signal_recog_particle_SRP54_M. [Graphical view]
Gene3D	G3DSA:1.20.120.140. SRP54_helical. 1 hit. G3DSA:1.10.260.30. SRP54_M. 1 hit.
PANTHER	PTHR11564:SF7. SRP_sub. 1 hit.
Pfam	PF00448. SRP54. 1 hit. PF02881. SRP54_N. 1 hit. PF02978. SRP_SPB. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00959. ffh. 1 hit.
PROSITE	PS00300. SRP54. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SRP54_MYCGE

Accession

Primary (citable) accession number: P47294

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 24, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)