Reviewed, UniProtKB/Swiss-Prot P47293 (METK_MYCGE)

Names and origin

Protein names

Recommended name:
    S-adenosylmethionine synthetase
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase
    AdoMet synthetase
    MAT

Gene names

Name:	metK
Synonyms:	metX
Ordered Locus Names:	MG047

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine. HAMAP MF_00086
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1. HAMAP MF_00086
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the AdoMet synthetase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP methionine adenosyltransferase activity Inferred from electronic annotation. Source: HAMAP potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	S-adenosylmethionine synthetase HAMAP MF_00086		PRO_0000174550
Regions
	Nucleotide binding	256 – 263	8	ATP Potential
Sites
	Metal binding	24	1	Magnesium By similarity
	Metal binding	50	1	Potassium By similarity
	Metal binding	260	1	Potassium By similarity
	Metal binding	268	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47293-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: F2340734FAA9EE16	FASTA	383	42,716
	10 20 30 40 50 60 MAIRIKSTRV GRFVSESVGL GHPDKICDQI ADSILDQCLL QSKTSHVACE VFASKNLILI 70 80 90 100 110 120 GGEISTSGYV DVVQTAWRIL RNLGYNETDF SFLSCINNQS LEINQAVLKN NEINAGDQGI 130 140 150 160 170 180 TVGYAVNETK QLMPLGVLLA HSFLKQAEKL TKQFDFLKND MKSQVVLNYS LNQVECEEVL 190 200 210 220 230 240 LSIQHTNAIS LTELRKVIEN NVILPVLNQY GFQDKKPTCL VNPGGSFVLG GPMADTGLTG 250 260 270 280 290 300 RKIIVDTYGP YAHHGGGSFS GKDPSKVDRT GAYFARFIAK HIVSLGWASE CEVSISWVFS 310 320 330 340 350 360 KPNPQSITVK CFNTNIQYDE VLINRVVNNY FNWSITKIID KLKLLDFVKY SDYAVYGHFG 370 380 NDLSPWEQPT ELDKLECLIK NFH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 263-357. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43967 Genomic DNA. Translation: AAC71263.1. U02123 Genomic DNA. Translation: AAD12398.1.
PIR	B64205.
RefSeq	NP_072707.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	875239.
GenomeReviews	Gene locus MG047 in contig L43967_GR.
KEGG	mge:MG_047.
TIGR	MG047.
Phylogenomic databases
HOGENOM	P47293.
OMA	LWQCFED
Enzyme and pathway databases
BioCyc	MGEN243273:MG_047-MON.
BRENDA	2.5.1.6. 110.
Family and domain databases
HAMAP	MF_00086. [Tree]
InterPro	IPR002133. S-AdoMet_synthetase. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHETASE_1. 1 hit. PS00377. ADOMET_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK_MYCGE

Accession

Primary (citable) accession number: P47293

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 24, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)