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P47292

- TSAD_MYCGE

UniProt

P47292 - TSAD_MYCGE

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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi110 – 1101IronUniRule annotation
Metal bindingi114 – 1141IronUniRule annotation
Binding sitei168 – 1681SubstrateUniRule annotation
Binding sitei181 – 1811Substrate; via amide nitrogenUniRule annotation
Binding sitei185 – 1851SubstrateUniRule annotation
Binding sitei273 – 2731SubstrateUniRule annotation
Metal bindingi297 – 2971IronUniRule annotation

GO - Molecular functioni

  1. iron ion binding Source: UniProtKB-HAMAP
  2. metalloendopeptidase activity Source: InterPro
  3. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-46-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:MG046
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000096967Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243273.MG_046.

Structurei

3D structure databases

ProteinModelPortaliP47292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni135 – 1395Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0533.
KOiK01409.
OMAiEVEKNAH.
OrthoDBiEOG6K402S.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47292-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEQPLCVLGI ETTCDDTGLS IVIDQKIKSN IVISSANLHV KTGGVVPEIA
60 70 80 90 100
ARCHEQNLFK AIRDLNFEIR DLSHIAYACN PGLAGCLHVG ATFARSLSFL
110 120 130 140 150
LDKPLLPINH LYAHIFSCLI DQDLNKLQLP ALGLVISGGH TAIYLVKSFY
160 170 180 190 200
ELELIAETSD DAIGEVYDKI GRAMGFDYPA GSKIDSLFNK ELVKPHYFFK
210 220 230 240 250
PSTKWTKFSY SGLKSQCLNK IKQISANKTR IDWSELASNF QATIIDHYID
260 270 280 290 300
HVKNAIKKFA PKMLLVGGGV SANSYLSNRI STLNLPFLIA DSKYTSDNGA
310
MIGFYASLLI NGDKN
Length:315
Mass (Da):34,708
Last modified:February 1, 1996 - v1
Checksum:iD186ECD176C4E574
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71262.1.
PIRiA64205.
RefSeqiNP_072706.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71262; AAC71262; MG_046.
GeneIDi875433.
KEGGimge:MG_046.
PATRICi20009470. VBIMycGen98045_0045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71262.1 .
PIRi A64205.
RefSeqi NP_072706.1. NC_000908.2.

3D structure databases

ProteinModelPortali P47292.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243273.MG_046.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC71262 ; AAC71262 ; MG_046 .
GeneIDi 875433.
KEGGi mge:MG_046.
PATRICi 20009470. VBIMycGen98045_0045.

Phylogenomic databases

eggNOGi COG0533.
KOi K01409.
OMAi EVEKNAH.
OrthoDBi EOG6K402S.

Enzyme and pathway databases

BioCyci MGEN243273:GH2R-46-MONOMER.

Family and domain databases

HAMAPi MF_01445. TsaD.
InterProi IPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view ]
Pfami PF00814. Peptidase_M22. 1 hit.
[Graphical view ]
PRINTSi PR00789. OSIALOPTASE.
TIGRFAMsi TIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiTSAD_MYCGE
AccessioniPrimary (citable) accession number: P47292
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3