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P47292 (TSAD_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA N6-adenosine threonylcarbamoyltransferase

EC=2.6.99.4
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name=t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD
tRNA threonylcarbamoyladenosine biosynthesis protein TsaD
Gene names
Name:tsaD
Synonyms:gcp
Ordered Locus Names:MG046
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction By similarity. HAMAP-Rule MF_01445

Catalytic activity

L-threonylcarbamoyladenylate + adenine37 in tRNA = AMP + N(6)-L-threonylcarbamoyladenine37 in tRNA. HAMAP-Rule MF_01445

Cofactor

Binds 1 Fe2+ ion per subunit By similarity. HAMAP-Rule MF_01445

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01445.

Sequence similarities

Belongs to the KAE1 / TsaD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandIron
Metal-binding
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processthreonylcarbamoyladenosine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioniron ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

transferase activity, transferring acyl groups other than amino-acyl groups

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315tRNA N6-adenosine threonylcarbamoyltransferase HAMAP-Rule MF_01445
PRO_0000096967

Regions

Region135 – 1395Substrate binding By similarity

Sites

Metal binding1101Iron By similarity
Metal binding1141Iron By similarity
Metal binding2971Iron By similarity
Binding site1681Substrate By similarity
Binding site1811Substrate; via amide nitrogen By similarity
Binding site1851Substrate By similarity
Binding site2731Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P47292 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: D186ECD176C4E574

FASTA31534,708
        10         20         30         40         50         60 
MEQPLCVLGI ETTCDDTGLS IVIDQKIKSN IVISSANLHV KTGGVVPEIA ARCHEQNLFK 

        70         80         90        100        110        120 
AIRDLNFEIR DLSHIAYACN PGLAGCLHVG ATFARSLSFL LDKPLLPINH LYAHIFSCLI 

       130        140        150        160        170        180 
DQDLNKLQLP ALGLVISGGH TAIYLVKSFY ELELIAETSD DAIGEVYDKI GRAMGFDYPA 

       190        200        210        220        230        240 
GSKIDSLFNK ELVKPHYFFK PSTKWTKFSY SGLKSQCLNK IKQISANKTR IDWSELASNF 

       250        260        270        280        290        300 
QATIIDHYID HVKNAIKKFA PKMLLVGGGV SANSYLSNRI STLNLPFLIA DSKYTSDNGA 

       310 
MIGFYASLLI NGDKN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC71262.1.
PIRA64205.
RefSeqNP_072706.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47292.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_046.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC71262; AAC71262; MG_046.
GeneID875433.
KEGGmge:MG_046.
PATRIC20009470. VBIMycGen98045_0045.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0533.
KOK01409.
OMAEVEKNAH.
OrthoDBEOG6K402S.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-46-MONOMER.

Family and domain databases

HAMAPMF_01445. TsaD.
InterProIPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSPR00789. OSIALOPTASE.
TIGRFAMsTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTSAD_MYCGE
AccessionPrimary (citable) accession number: P47292
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names