SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P47292

- TSAD_MYCGE

UniProt

P47292 - TSAD_MYCGE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene
tsaD, gcp, MG046
Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction By similarity.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi110 – 1101Iron By similarity
Metal bindingi114 – 1141Iron By similarity
Binding sitei168 – 1681Substrate By similarity
Binding sitei181 – 1811Substrate; via amide nitrogen By similarity
Binding sitei185 – 1851Substrate By similarity
Binding sitei273 – 2731Substrate By similarity
Metal bindingi297 – 2971Iron By similarity

GO - Molecular functioni

  1. iron ion binding Source: UniProtKB-HAMAP
  2. metalloendopeptidase activity Source: InterPro
  3. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-46-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferase (EC:2.6.99.4)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD
tRNA threonylcarbamoyladenosine biosynthesis protein TsaD
Gene namesi
Name:tsaD
Synonyms:gcp
Ordered Locus Names:MG046
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotationPRO_0000096967Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243273.MG_046.

Structurei

3D structure databases

ProteinModelPortaliP47292.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni135 – 1395Substrate binding By similarity

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.

Phylogenomic databases

eggNOGiCOG0533.
KOiK01409.
OMAiEVEKNAH.
OrthoDBiEOG6K402S.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47292-1 [UniParc]FASTAAdd to Basket

« Hide

MEQPLCVLGI ETTCDDTGLS IVIDQKIKSN IVISSANLHV KTGGVVPEIA    50
ARCHEQNLFK AIRDLNFEIR DLSHIAYACN PGLAGCLHVG ATFARSLSFL 100
LDKPLLPINH LYAHIFSCLI DQDLNKLQLP ALGLVISGGH TAIYLVKSFY 150
ELELIAETSD DAIGEVYDKI GRAMGFDYPA GSKIDSLFNK ELVKPHYFFK 200
PSTKWTKFSY SGLKSQCLNK IKQISANKTR IDWSELASNF QATIIDHYID 250
HVKNAIKKFA PKMLLVGGGV SANSYLSNRI STLNLPFLIA DSKYTSDNGA 300
MIGFYASLLI NGDKN 315
Length:315
Mass (Da):34,708
Last modified:February 1, 1996 - v1
Checksum:iD186ECD176C4E574
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71262.1.
PIRiA64205.
RefSeqiNP_072706.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71262; AAC71262; MG_046.
GeneIDi875433.
KEGGimge:MG_046.
PATRICi20009470. VBIMycGen98045_0045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43967 Genomic DNA. Translation: AAC71262.1 .
PIRi A64205.
RefSeqi NP_072706.1. NC_000908.2.

3D structure databases

ProteinModelPortali P47292.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243273.MG_046.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC71262 ; AAC71262 ; MG_046 .
GeneIDi 875433.
KEGGi mge:MG_046.
PATRICi 20009470. VBIMycGen98045_0045.

Phylogenomic databases

eggNOGi COG0533.
KOi K01409.
OMAi EVEKNAH.
OrthoDBi EOG6K402S.

Enzyme and pathway databases

BioCyci MGEN243273:GH2R-46-MONOMER.

Family and domain databases

HAMAPi MF_01445. TsaD.
InterProi IPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view ]
Pfami PF00814. Peptidase_M22. 1 hit.
[Graphical view ]
PRINTSi PR00789. OSIALOPTASE.
TIGRFAMsi TIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiTSAD_MYCGE
AccessioniPrimary (citable) accession number: P47292
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi