Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P47292

- TSAD_MYCGE

UniProt

P47292 - TSAD_MYCGE

Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

    Catalytic activityi

    L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

    Cofactori

    Binds 1 Fe2+ ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi110 – 1101IronUniRule annotation
    Metal bindingi114 – 1141IronUniRule annotation
    Binding sitei168 – 1681SubstrateUniRule annotation
    Binding sitei181 – 1811Substrate; via amide nitrogenUniRule annotation
    Binding sitei185 – 1851SubstrateUniRule annotation
    Binding sitei273 – 2731SubstrateUniRule annotation
    Metal bindingi297 – 2971IronUniRule annotation

    GO - Molecular functioni

    1. iron ion binding Source: UniProtKB-HAMAP
    2. metalloendopeptidase activity Source: InterPro
    3. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP

    GO - Biological processi

    1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciMGEN243273:GH2R-46-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthaseUniRule annotation
    Short name:
    t(6)A synthaseUniRule annotation
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
    tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
    Gene namesi
    Name:tsaDUniRule annotation
    Synonyms:gcp
    Ordered Locus Names:MG046
    OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
    Taxonomic identifieri243273 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000807: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 315315tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000096967Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi243273.MG_046.

    Structurei

    3D structure databases

    ProteinModelPortaliP47292.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni135 – 1395Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the KAE1 / TsaD family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0533.
    KOiK01409.
    OMAiEVEKNAH.
    OrthoDBiEOG6K402S.

    Family and domain databases

    HAMAPiMF_01445. TsaD.
    InterProiIPR000905. Gcp-like_dom.
    IPR017861. KAE1/YgjD.
    IPR017860. Peptidase_M22_CS.
    IPR022450. TsaD.
    [Graphical view]
    PfamiPF00814. Peptidase_M22. 1 hit.
    [Graphical view]
    PRINTSiPR00789. OSIALOPTASE.
    TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
    TIGR03723. T6A_YgjD. 1 hit.
    PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47292-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQPLCVLGI ETTCDDTGLS IVIDQKIKSN IVISSANLHV KTGGVVPEIA    50
    ARCHEQNLFK AIRDLNFEIR DLSHIAYACN PGLAGCLHVG ATFARSLSFL 100
    LDKPLLPINH LYAHIFSCLI DQDLNKLQLP ALGLVISGGH TAIYLVKSFY 150
    ELELIAETSD DAIGEVYDKI GRAMGFDYPA GSKIDSLFNK ELVKPHYFFK 200
    PSTKWTKFSY SGLKSQCLNK IKQISANKTR IDWSELASNF QATIIDHYID 250
    HVKNAIKKFA PKMLLVGGGV SANSYLSNRI STLNLPFLIA DSKYTSDNGA 300
    MIGFYASLLI NGDKN 315
    Length:315
    Mass (Da):34,708
    Last modified:February 1, 1996 - v1
    Checksum:iD186ECD176C4E574
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71262.1.
    PIRiA64205.
    RefSeqiNP_072706.1. NC_000908.2.

    Genome annotation databases

    EnsemblBacteriaiAAC71262; AAC71262; MG_046.
    GeneIDi875433.
    KEGGimge:MG_046.
    PATRICi20009470. VBIMycGen98045_0045.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71262.1 .
    PIRi A64205.
    RefSeqi NP_072706.1. NC_000908.2.

    3D structure databases

    ProteinModelPortali P47292.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243273.MG_046.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC71262 ; AAC71262 ; MG_046 .
    GeneIDi 875433.
    KEGGi mge:MG_046.
    PATRICi 20009470. VBIMycGen98045_0045.

    Phylogenomic databases

    eggNOGi COG0533.
    KOi K01409.
    OMAi EVEKNAH.
    OrthoDBi EOG6K402S.

    Enzyme and pathway databases

    BioCyci MGEN243273:GH2R-46-MONOMER.

    Family and domain databases

    HAMAPi MF_01445. TsaD.
    InterProi IPR000905. Gcp-like_dom.
    IPR017861. KAE1/YgjD.
    IPR017860. Peptidase_M22_CS.
    IPR022450. TsaD.
    [Graphical view ]
    Pfami PF00814. Peptidase_M22. 1 hit.
    [Graphical view ]
    PRINTSi PR00789. OSIALOPTASE.
    TIGRFAMsi TIGR00329. gcp_kae1. 1 hit.
    TIGR03723. T6A_YgjD. 1 hit.
    PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33530 / G-37 / NCTC 10195.

    Entry informationi

    Entry nameiTSAD_MYCGE
    AccessioniPrimary (citable) accession number: P47292
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Mycoplasma genitalium
      Mycoplasma genitalium (strain G-37): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3